Recombinant Human GASP-1/WFIKKN2 Protein | R&D Systems 2070-GS-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human GASP-1/WFIKKN2 Protein

catalog :

2070-GS-025

quantity :

25 ug

price :

432 USD

more info or order :

R&D Systems product webpage

citations: 2

Reference
Nickerson K, Tom I, Cort xe9 s E, Abolafia J, xd6 zkan E, Gonzalez L, et al. WFIKKN2 is a bifunctional axon guidance cue that signals through divergent DCC family receptors. bioRxiv. 2023;: pubmed publisher
Walker R, Czepnik M, Goebel E, McCoy J, Vujic A, Cho M, et al. Structural basis for potency differences between GDF8 and GDF11. BMC Biol. 2017;15:19 pubmed publisher

product information

master code :

2070-GS

SKU :

2070-GS-025

product name :

Recombinant Human GASP-1/WFIKKN2 Protein

unit size :

25 ug

description :

The Recombinant Human GASP-1/WFIKKN2 Protein from R&D Systems is derived from NS0. The Recombinant Human GASP-1/WFIKKN2 Protein has been validated for the following applications: Bioactivity, Inhibition Activity.

target :

GASP-1/WFIKKN2

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

75-80 kDa, reducing conditions

theoretical molecular weight :

60.7 kDa

gene symbol :

WFIKKN2

details of functionality :

Measured by its ability to inhibit rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.03-0.15 ╡g/mL, in the presence of 40 ng/mL of rmGDF-8. Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002 ). The IC50 value is approximately 1.5 nM, as measured in a reaction mixture containing 1.0 nM trypsin,10 mM ES002, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q8TEU8

applications :

Bioactivity, Inhibition Activity

source long :

Mouse myeloma cell line, NS0-derived human GASP-1/WFIKKN2 protein Leu35-His576, with a C-terminal 6-His tag

source short :

NS0

USD :

432 USD

alt names :

GASP1, GASP-1, Growth and differentiation factor-associated serum protein 1, hGASP-1, WAP, follistatin, immunoglobulin, kunitz and NTR domain-containing-relatedprotein, WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2, WAP, FS, Ig, two KU and NTR module related protein, WAP, kazal, immunoglobulin, kunitz and NTR domain-containing protein 2, WFIKKN2, WFIKKN-related protein, WFIKKNRP, WFIKKNRPmultivalent protease inhibitor protein

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage