Recombinant Human IL-5 Protein, CF | R&D Systems 205-IL-005/CF product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human IL-5 Protein, CF

catalog :

205-IL-005/CF

quantity :

5 ug (also 25 ug)

price :

219 USD

citations: 38

Reference
Hernandez J, Li T, Ghannam H, Rau C, Masuda M, Madura J, et al. Linking adipose tissue eosinophils, IL-4, and leptin in human obesity and insulin resistance. JCI Insight. 2024;9: pubmed publisher
Mejia R, Bennuru S, Oksov Y, Lustigman S, Munirathinam G, Kalyanasundaram R, et al. Localization and RNA Interference-Driven Inhibition of a Brugia malayi-Encoded Interleukin-5 Receptor Binding Protein. Infect Immun. 2022;90:e0031721 pubmed publisher
Imoto Y, Takabayashi T, Sakashita M, Kato Y, Yoshida K, Kidoguchi M, et al. Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis. Biomolecules. 2020;10: pubmed publisher
Yan J, Pandey S, Barnes B, Turner J, Abraham C. T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation. Cell Rep. 2020;31:107820 pubmed publisher
Cao Y, Shin S, Carroll D, O Sullivan J, Bochner B. Single-site, five-year experience with human eosinophil isolation by density gradient centrifugation and CD16 immunomagnetic negative separation. BMC Res Notes. 2020;13:211 pubmed publisher
Coden M, Loffredo L, Walker M, Jeong B, Nam K, Bochner B, et al. Fibrinogen Is a Specific Trigger for Cytolytic Eosinophil Degranulation. J Immunol. 2020;204:438-448 pubmed publisher
Qin X, Liu M, Zhang S, Wang C, Zhang T. The role of IL-36γ and its regulation in eosinophilic inflammation in allergic rhinitis. Cytokine. 2019;117:84-90 pubmed publisher
Barretto K, Swanson C, Nguyen C, Annis D, Esnault S, Mosher D, et al. Control of cytokine-driven eosinophil migratory behavior by TGF-beta-induced protein (TGFBI) and periostin. PLoS ONE. 2018;13:e0201320 pubmed publisher
Bortnov V, Annis D, FOGERTY F, Barretto K, Turton K, Mosher D. Myeloid-derived growth factor is a resident endoplasmic reticulum protein. J Biol Chem. 2018;293:13166-13175 pubmed publisher
Bucher F, Lee J, Shin S, Kim M, Oh Y, Ha S, et al. Interleukin-5 suppresses Vascular Endothelial Growth Factor-induced angiogenesis through STAT5 signaling. Cytokine. 2018;110:397-403 pubmed publisher
Zellweger F, Buschor P, Hobi G, Brigger D, Dahinden C, Villiger P, et al. IL-3 but not monomeric IgE regulates Fc?RI levels and cell survival in primary human basophils. Cell Death Dis. 2018;9:510 pubmed publisher
Schroeder J, Bieneman A. Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen. J Immunol. 2017;199:855-865 pubmed publisher
Johansson M, Khanna M, Bortnov V, Annis D, Nguyen C, Mosher D. IL-5-stimulated eosinophils adherent to periostin undergo stereotypic morphological changes and ADAM8-dependent migration. Clin Exp Allergy. 2017;47:1263-1274 pubmed publisher
Toyama S, Okada N, Matsuda A, Morita H, Saito H, Fujisawa T, et al. Human eosinophils constitutively express a unique serine protease, PRSS33. Allergol Int. 2017;66:463-471 pubmed publisher
Tsuzuki H, Arinobu Y, Miyawaki K, Takaki A, Ota S, Ota Y, et al. Functional interleukin-33 receptors are expressed in early progenitor stages of allergy-related granulocytes. Immunology. 2017;150:64-73 pubmed publisher
Yamauchi Y, Ueki S, Konno Y, Ito W, Takeda M, Nakamura Y, et al. The effect of hepatocyte growth factor on secretory functions in human eosinophils. Cytokine. 2016;88:45-50 pubmed publisher
Wright A, Mahaut Smith M, Symon F, Sylvius N, Ran S, Bafadhel M, et al. Impaired P2X1 Receptor-Mediated Adhesion in Eosinophils from Asthmatic Patients. J Immunol. 2016;196:4877-84 pubmed publisher
Yao X, Wang W, Li Y, Huang P, Zhang Q, Wang J, et al. IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model. Respir Res. 2015;16:39 pubmed publisher
Schwartz C, Willebrand R, Huber S, Rupec R, WU D, Locksley R, et al. Eosinophil-specific deletion of IÎºBÎ± in mice reveals a critical role of NF-ÎºB-induced Bcl-xL for inhibition of apoptosis. Blood. 2015;125:3896-904 pubmed publisher
Konno Y, Ueki S, Takeda M, Kobayashi Y, Tamaki M, Moritoki Y, et al. Functional analysis of free fatty acid receptor GPR120 in human eosinophils: implications in metabolic homeostasis. PLoS ONE. 2015;10:e0120386 pubmed publisher
Dyer K, Rosenberg H. Physiologic concentrations of HMGB1 have no impact on cytokine-mediated eosinophil survival or chemotaxis in response to Eotaxin-2 (CCL24). PLoS ONE. 2015;10:e0118887 pubmed publisher
Zafra M, CaÃ±as J, Mazzeo C, GÃ¡mez C, Sanz V, FernÃ¡ndez Nieto M, et al. SOCS3 silencing attenuates eosinophil functions in asthma patients. Int J Mol Sci. 2015;16:5434-51 pubmed publisher
Burnham M, Esnault S, Roti Roti E, Bates M, Bertics P, Denlinger L. Cholesterol selectively regulates IL-5 induced mitogen activated protein kinase signaling in human eosinophils. PLoS ONE. 2014;9:e103122 pubmed publisher
Awad A, Yassine H, Barrier M, Vorng H, Marquillies P, Tsicopoulos A, et al. Natural killer cells induce eosinophil activation and apoptosis. PLoS ONE. 2014;9:e94492 pubmed publisher
Kankaanranta H, Ilmarinen P, Zhang X, Adcock I, Lahti A, Barnes P, et al. Tumour necrosis factor-? regulates human eosinophil apoptosis via ligation of TNF-receptor 1 and balance between NF-?B and AP-1. PLoS ONE. 2014;9:e90298 pubmed publisher
Suzuki H, Raska M, Yamada K, Moldoveanu Z, Julian B, Wyatt R, et al. Cytokines alter IgA1 O-glycosylation by dysregulating C1GalT1 and ST6GalNAc-II enzymes. J Biol Chem. 2014;289:5330-9 pubmed publisher
Oh J, Malter J. Pin1-FADD interactions regulate Fas-mediated apoptosis in activated eosinophils. J Immunol. 2013;190:4937-45 pubmed publisher
Puxeddu I, Berkman N, Ribatti D, Bader R, Haitchi H, Davies D, et al. Osteopontin is expressed and functional in human eosinophils. Allergy. 2010;65:168-74 pubmed publisher
Qui H, Ferrell E, Nolan N, Phelps B, Tabibiazar R, Whitney D, et al. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin Chem. 2007;53:2010-2 pubmed
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck L, et al. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin Immunol. 2006;120:220-31 pubmed
Vassina E, Yousefi S, Simon D, Zwicky C, Conus S, Simon H. cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis. Eur J Immunol. 2006;36:1975-84 pubmed
Brooks A, Bates M, Vrtis R, Jarjour N, Bertics P, Sedgwick J. Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma. Am J Respir Cell Mol Biol. 2006;35:503-11 pubmed
Liu F, Gonzalo J, Manning S, O Connell L, Fedyk E, Burke K, et al. Pharmacological characterization of guinea pig chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2). Prostaglandins Other Lipid Mediat. 2005;76:133-47 pubmed
Fujitsu Y, Fukuda K, Kimura K, Seki K, Kumagai N, Nishida T. Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13. Invest Ophthalmol Vis Sci. 2005;46:797-802 pubmed
Kao C, Chen Y, Thai P, Wachi S, Huang F, Kim C, et al. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways. J Immunol. 2004;173:3482-91 pubmed
Johansson M, Lye M, Barthel S, Duffy A, Annis D, Mosher D. Eosinophils adhere to vascular cell adhesion molecule-1 via podosomes. Am J Respir Cell Mol Biol. 2004;31:413-22 pubmed
Hellman C, Hallden G, Hylander B, Lundahl J. Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects. Clin Exp Immunol. 2003;131:75-81 pubmed
Liu X, Kohyama T, Wang H, Zhu Y, Wen F, Kim H, et al. Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells. Am J Physiol Lung Cell Mol Physiol. 2002;282:L1049-56 pubmed

product information

master code :

205-IL/CF

SKU :

205-IL-005/CF

product name :

Recombinant Human IL-5 Protein, CF

unit size :

5 ug (also 25 ug)

description :

The Recombinant Human IL-5 Protein, CF from R&D Systems is derived from Sf 21 (stably transfected). The Recombinant Human IL-5 Protein, CF has been validated for the following applications: Bioactivity.

target :

IL-5

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

13-15 kDa, reducing conditions

theoretical molecular weight :

13.1 kDa (monomer)

gene symbol :

IL5

details of functionality :

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al . (1989) J. Cell Physiol. 140 :323. The ED50 for this effect is 0.04-0.2 ng/mL.

endotoxin note :

<0.01 EU per 1 ╡g of the protein by the LAL method.

top caption :

Recombinant Human IL-5 Protein Bioactivity

accessionNumbers :

P05113

applications :

Bioactivity

source long :

Spodoptera frugiperda , Sf 21 (stably transfected)-derived human IL-5 protein Ile20-Ser134

source short :

Sf 21 (stably transfected)

USD :

219 USD

alt names :

BCDF mu, B-cell differentiation factor I, BCGFII, EDF, Eo-CSF, Eosinophil differentiation factor, IL5, IL-5T-cell replacing factor, interleukin 5 (colony-stimulating factor, eosinophil), interleukin-5, TRF, TRFB cell differentiation factor I

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after opening. 3 months, -20 to -70 ░C under sterile conditions after opening.