product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Complement Component C5a Protein, CF
catalog :
2037-C5-025/CF
quantity :
25 ug
price :
365 USD
more info or order :
R&D Systems product webpage
citations: 22
Reference
Plant Hately A, Eryilmaz B, David C, Brain D, Heaton B, Perrie Y, et al. Exposure of the Basophilic Cell Line KU812 to Liposomes Reveals Activation Profiles Associated with Potential Anaphylactic Responses Linked to Physico-Chemical Characteristics. Pharmaceutics. 2022;14: pubmed publisher
Liu J, Wei X, Hu J, Tan X, Kang X, Gao L, et al. Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation. BMC Oral Health. 2021;21:470 pubmed publisher
Medica D, Franzin R, Stasi A, Castellano G, Migliori M, Panichi V, et al. Extracellular Vesicles Derived from Endothelial Progenitor Cells Protect Human Glomerular Endothelial Cells and Podocytes from Complement- and Cytokine-Mediated Injury. Cells. 2021;10: pubmed publisher
Silawal S, Kohl B, Shi J, Schulze Tanzil G. Complement Regulation in Human Tenocytes under the Influence of Anaphylatoxin C5a. Int J Mol Sci. 2021;22: pubmed publisher
Kashiwagi N, Saito F, Maegawa H, Kaneda K. Granulocyte and monocyte adsorptive apheresis induces apoptosis of neutrophils and release of a novel chemoattractant for desensitization of interleukin-8 response - In vitro and in vivo results. Cytokine. 2021;139:155410 pubmed publisher
Giraldo Calderón G, Calle tobÓn A, Rozo Lopez P, Colpitts T, Park Y, Rúa Uribe G, et al. Transcriptome of the Aedes aegypti Mosquito in Response to Human Complement Proteins. Int J Mol Sci. 2020;21: pubmed publisher
Hong L, Tang Y, Pan S, Xu M, Shi Y, Gao S, et al. Interleukin 3-induced GITR promotes the activation of human basophils. Cytokine. 2020;136:155268 pubmed publisher
Ohlsson S, Holm L, Hansson C, Ohlsson S, Gunnarsson L, Pettersson A, et al. Neutrophils from ANCA-associated vasculitis patients show an increased capacity to activate the complement system via the alternative pathway after ANCA stimulation. PLoS ONE. 2019;14:e0218272 pubmed publisher
Paupert J, Espinosa E, Cenac N, Robert V, Laharrague P, Evrard S, et al. Rapid and Efficient Production of Human Functional Mast Cells through a Three-Dimensional Culture of Adipose Tissue-Derived Stromal Vascular Cells. J Immunol. 2018;201:3815-3821 pubmed publisher
Zhang Y, Cao Y, Zhang L, Feng C, Zhou G, Wen G. Apigenin inhibits C5a-induced proliferation of human nasopharyngeal carcinoma cells through down-regulation of C5aR. Biosci Rep. 2018;38: pubmed publisher
Gruda M, Ruggeberg K, O Sullivan P, Guliashvili T, Scheirer A, Golobish T, et al. Broad adsorption of sepsis-related PAMP and DAMP molecules, mycotoxins, and cytokines from whole blood using CytoSorb® sorbent porous polymer beads. PLoS ONE. 2018;13:e0191676 pubmed publisher
Malard B, Florens N, Lambert C, Juillard L. Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study. Artif Organs. 2017;41:545-555 pubmed publisher
Lim E, Suh Y, Yoo K, Lee J, Kim I, Kim M, et al. Tumor-associated mesenchymal stem-like cells provide extracellular signaling cue for invasiveness of glioblastoma cells. Oncotarget. 2017;8:1438-1448 pubmed publisher
Chmilewsky F, Ayaz W, Appiah J, About I, Chung S. Nerve Growth Factor Secretion From Pulp Fibroblasts is Modulated by Complement C5a Receptor and Implied in Neurite Outgrowth. Sci Rep. 2016;6:31799 pubmed publisher
Yatime L, Maasch C, Hoehlig K, Klussmann S, Andersen G, Vater A. Structural basis for the targeting of complement anaphylatoxin C5a using a mixed L-RNA/L-DNA aptamer. Nat Commun. 2015;6:6481 pubmed publisher
Chmilewsky F, Jeanneau C, Laurent P, About I. Pulp fibroblasts synthesize functional complement proteins involved in initiating dentin-pulp regeneration. Am J Pathol. 2014;184:1991-2000 pubmed publisher
Jensen B, Frandsen P, Raaby E, Schiøtz P, Skov P, Poulsen L. Molecular and stimulus-response profiles illustrate heterogeneity between peripheral and cord blood-derived human mast cells. J Leukoc Biol. 2014;95:893-901 pubmed publisher
Hoehlig K, Maasch C, Shushakova N, Buchner K, Huber Lang M, Purschke W, et al. A novel C5a-neutralizing mirror-image (l-)aptamer prevents organ failure and improves survival in experimental sepsis. Mol Ther. 2013;21:2236-46 pubmed publisher
Liu B, Wei L, Meyerle C, Tuo J, Sen H, Li Z, et al. Complement component C5a promotes expression of IL-22 and IL-17 from human T cells and its implication in age-related macular degeneration. J Transl Med. 2011;9:1-12 pubmed publisher
Ruan B, Li X, Winkler A, Cunningham K, Kuai J, Greco R, et al. Complement C3a, CpG oligos, and DNA/C3a complex stimulate IFN-? production in a receptor for advanced glycation end product-dependent manner. J Immunol. 2010;185:4213-22 pubmed publisher
Truman L, Ford C, Pasikowska M, Pound J, Wilkinson S, Dumitriu I, et al. CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis. Blood. 2008;112:5026-36 pubmed publisher
Rittirsch D, Flierl M, Nadeau B, Day D, Huber Lang M, Mackay C, et al. Functional roles for C5a receptors in sepsis. Nat Med. 2008;14:551-7 pubmed publisher
product information
brand :
R&D Systems, a Bio-Techne Brand
catalog number base :
2037-C5/CF
SKU :
2037-C5-025/CF
product name :
Recombinant Human Complement Component C5a Protein, CF
Description :
The Recombinant Human Complement Component C5a Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human Complement Component C5a Protein, CF has been validated for the following applications: Bioactivity.
target :
Complement Component C5a
category :
Proteins and Enzymes
unit size :
25 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
theoretical molecular weight :
8.3 kDa
gene symbol :
C5
details of functionality :
Measured by its ability to induce N-acetyl-beta-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Gerard, NP and Gerard, C. (1990) Biochemistry 29: 9274. The ED50 for this effect is 1.5-5 ng/mL.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
P01031
applications :
Bioactivity
source :
E. coli-derived Recombinant Human Complement Component C5a Protein
USD :
365 USD
alt names :
C5, C5A, HE
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.