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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human IL-1 alpha/IL-1F1 Protein
catalog :
200-LA-002
quantity :
2 ug (also 10 ug)
price :
207 USD
more info or order :
citations: 64
Reference
Dovrolis N, Valatas V, Drygiannakis I, Filidou E, Spathakis M, Kandilogiannakis L, et al. Landscape of Interactions between Stromal and Myeloid Cells in Ileal Crohn's Disease; Indications of an Important Role for Fibroblast-Derived CCL-2. Biomedicines. 2024;12: pubmed publisher
Cheng Y, Acedera J, Li Y, Shieh S. A keratinocyte-adipocyte signaling loop is reprogrammed by loss of BTG3 to augment skin carcinogenesis. Cell Death Differ. 2024;31:970-982 pubmed publisher
Alves J, SCHWINN M, Machleidt T, Goueli S, Cali J, Zegzouti H. Monitoring phosphorylation and acetylation of CRISPR-mediated HiBiT-tagged endogenous proteins. Sci Rep. 2024;14:2138 pubmed publisher
Farías Jofré M, Romero R, Galaz J, Xu Y, Miller D, Garcia Flores V, et al. Blockade of IL-6R prevents preterm birth and adverse neonatal outcomes. EBioMedicine. 2023;98:104865 pubmed publisher
Grabarczyk M, Ksiazek Winiarek D, Glabinski A, Szpakowski P. Dietary Polyphenols Decrease Chemokine Release by Human Primary Astrocytes Responding to Pro-Inflammatory Cytokines. Pharmaceutics. 2023;15: pubmed publisher
Karema Jokinen V, Koskela A, Hytti M, Hongisto H, Viheri xe4 l xe4 T, Liukkonen M, et al. Crosstalk of protein clearance, inflammasome, and Ca2+ channels in retinal pigment epithelium derived from age-related macular degeneration patients. J Biol Chem. 2023;299:104770 pubmed publisher
Szpakowski P, Ksiazek Winiarek D, Turniak Kusy M, Pacan I, Glabinski A. Human Primary Astrocytes Differently Respond to Pro- and Anti-Inflammatory Stimuli. Biomedicines. 2022;10: pubmed publisher
Ebeling M, Fisher C, Kapphahn R, Stahl M, Shen S, Qu J, et al. Inflammasome Activation in Retinal Pigment Epithelium from Human Donors with Age-Related Macular Degeneration. Cells. 2022;11: pubmed publisher
Filidou E, Kandilogiannakis L, Tarapatzi G, Spathakis M, Steiropoulos P, Mikroulis D, et al. Anti-Inflammatory and Anti-Fibrotic Effect of Immortalized Mesenchymal-Stem-Cell-Derived Conditioned Medium on Human Lung Myofibroblasts and Epithelial Cells. Int J Mol Sci. 2022;23: pubmed publisher
Zhu Y, Prata L, Gerdes E, Netto J, Pirtskhalava T, Giorgadze N, et al. Orally-active, clinically-translatable senolytics restore α-Klotho in mice and humans. EBioMedicine. 2022;77:103912 pubmed publisher
McAleavy M, Zhang Q, Ehmann P, Xu J, Wipperman M, Ajithdoss D, et al. The Activin/FLRG Pathway Associates with Poor COVID-19 Outcomes in Hospitalized Patients. Mol Cell Biol. 2022;42:e0046721 pubmed publisher
Sheldon H, Alexander J, Bridges E, Moreira L, Reilly S, Ang K, et al. ELTD1 Activation Induces an Endothelial-EMT Transition to a Myofibroblast Phenotype. Int J Mol Sci. 2021;22: pubmed publisher
Biavasco R, Lettera E, Giannetti K, Gilioli D, Beretta S, Conti A, et al. Oncogene-induced senescence in hematopoietic progenitors features myeloid restricted hematopoiesis, chronic inflammation and histiocytosis. Nat Commun. 2021;12:4559 pubmed publisher
Bhattarai N, Korhonen E, Mysore Y, Kaarniranta K, Kauppinen A. Hydroquinone Induces NLRP3-Independent IL-18 Release from ARPE-19 Cells. Cells. 2021;10: pubmed publisher
Camell C, Yousefzadeh M, Zhu Y, Langhi Prata L, Huggins M, Pierson M, et al. Senolytics reduce coronavirus-related mortality in old mice. Science. 2021;: pubmed publisher
Ranta Aho S, Piippo N, Korhonen E, Kaarniranta K, Hytti M, Kauppinen A. TAS-116, a Well-Tolerated Hsp90 Inhibitor, Prevents the Activation of the NLRP3 Inflammasome in Human Retinal Pigment Epithelial Cells. Int J Mol Sci. 2021;22: pubmed publisher
Hayn M, Hirschenberger M, Koepke L, Nchioua R, Straub J, Klute S, et al. Systematic functional analysis of SARS-CoV-2 proteins uncovers viral innate immune antagonists and remaining vulnerabilities. Cell Rep. 2021;35:109126 pubmed publisher
Bernau K, Leet J, Floerke H, Bruhn E, Noll A, McDermott I, et al. Interleukin-1α Is a Critical Mediator of the Response of Human Bronchial Fibroblasts to Eosinophilic Inflammation. Cells. 2021;10: pubmed publisher
Li X, Yin G, Ji W, Liu J, Zhang Y, Wang J, et al. 18F-FHBG PET-CT Reporter Gene Imaging of Adoptive CIK Cell Transfer Immunotherapy for Breast Cancer in a Mouse Model. Onco Targets Ther. 2020;13:11659-11668 pubmed publisher
Liu D, Steins A, Klaassen R, van der Zalm A, Bennink R, van Tienhoven G, et al. Soluble Compounds Released by Hypoxic Stroma Confer Invasive Properties to Pancreatic Ductal Adenocarcinoma. Biomedicines. 2020;8: pubmed publisher
Sun J, LaRock D, Skowronski E, Kimmey J, Olson J, Jiang Z, et al. The Pseudomonas aeruginosa protease LasB directly activates IL-1β. EBioMedicine. 2020;60:102984 pubmed publisher
Cunningham C, Wong R, Barrington J, Tamburrano S, Pinteaux E, Allan S. Systemic conditioned medium treatment from interleukin-1 primed mesenchymal stem cells promotes recovery after stroke. Stem Cell Res Ther. 2020;11:32 pubmed publisher
Powers N, Swartzwelter B, Marchetti C, de Graaf D, Lerchner A, Schlapschy M, et al. PASylation of IL-1 receptor antagonist (IL-1Ra) retains IL-1 blockade and extends its duration in mouse urate crystal-induced peritonitis. J Biol Chem. 2020;295:868-882 pubmed publisher
Piippo N, Korhonen E, Hytti M, Kinnunen K, Kaarniranta K, Kauppinen A. Oxidative Stress is the Principal Contributor to Inflammasome Activation in Retinal Pigment Epithelium Cells with Defunct Proteasomes and Autophagy. Cell Physiol Biochem. 2018;49:359-367 pubmed publisher
Ravindra K, Ahrens C, Wang Y, Ramseier J, Wishnok J, Griffith L, et al. Chemoproteomics of matrix metalloproteases in a model of cartilage degeneration suggests functional biomarkers associated with posttraumatic osteoarthritis. J Biol Chem. 2018;293:11459-11469 pubmed publisher
Couderc E, Morel F, Levillain P, Buffiere Morgado A, Camus M, Paquier C, et al. Interleukin-17A-induced production of acute serum amyloid A by keratinocytes contributes to psoriasis pathogenesis. PLoS ONE. 2017;12:e0181486 pubmed publisher
von Stemann J, Rigas A, Thørner L, Rasmussen D, Pedersen O, Rostgaard K, et al. Prevalence and correlation of cytokine-specific autoantibodies with epidemiological factors and C-reactive protein in 8,972 healthy individuals: Results from the Danish Blood Donor Study. PLoS ONE. 2017;12:e0179981 pubmed publisher
Tian B, Al Moujahed A, Bouzika P, Hu Y, Notomi S, Tsoka P, et al. Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells. Sci Rep. 2017;7:2329 pubmed publisher
Redondo Castro E, Cunningham C, Miller J, Martuscelli L, Aoulad Ali S, Rothwell N, et al. Interleukin-1 primes human mesenchymal stem cells towards an anti-inflammatory and pro-trophic phenotype in vitro. Stem Cell Res Ther. 2017;8:79 pubmed publisher
Perrott K, Wiley C, Desprez P, Campisi J. Apigenin suppresses the senescence-associated secretory phenotype and paracrine effects on breast cancer cells. GeroScience. 2017;39:161-173 pubmed publisher
Choi S, Chung H, Hong H, Kim S, Kim S, Seoh J, et al. Inflammatory hypoxia induces syndecan-2 expression through IL-1?-mediated FOXO3a activation in colonic epithelia. FASEB J. 2017;31:1516-1530 pubmed publisher
Savill S, Leitch H, Harvey J, Thomas T. Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients. PLoS ONE. 2016;11:e0162908 pubmed publisher
Ballantyne M, Pinel K, Dakin R, Vesey A, Diver L, Mackenzie R, et al. Smooth Muscle Enriched Long Noncoding RNA (SMILR) Regulates Cell Proliferation. Circulation. 2016;133:2050-65 pubmed publisher
Robert S, Gicquel T, Bodin A, Lagente V, Boichot E. Characterization of the MMP/TIMP Imbalance and Collagen Production Induced by IL-1β or TNF-α Release from Human Hepatic Stellate Cells. PLoS ONE. 2016;11:e0153118 pubmed publisher
Dankbar B, Fennen M, Brunert D, Hayer S, Frank S, Wehmeyer C, et al. Myostatin is a direct regulator of osteoclast differentiation and its inhibition reduces inflammatory joint destruction in mice. Nat Med. 2015;21:1085-90 pubmed publisher
Fernandez Bustamante A, Agazio A, Wilson P, Elkins N, Domaleski L, He Q, et al. Brief Glutamine Pretreatment Increases Alveolar Macrophage CD163/Heme Oxygenase-1/p38-MAPK Dephosphorylation Pathway and Decreases Capillary Damage but Not Neutrophil Recruitment in IL-1/LPS-Insufflated Rats. PLoS ONE. 2015;10:e0130764 pubmed publisher
Laberge R, Sun Y, Orjalo A, Patil C, Freund A, Zhou L, et al. MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation. Nat Cell Biol. 2015;17:1049-61 pubmed publisher
Rabeony H, Petit Paris I, Garnier J, Barrault C, Pedretti N, Guilloteau K, et al. Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1?, TNF? and oncostatin M. PLoS ONE. 2014;9:e101937 pubmed publisher
Gschwandtner M, Zhong S, Tschachler A, Mlitz V, Karner S, Elbe Bürger A, et al. Fetal human keratinocytes produce large amounts of antimicrobial peptides: involvement of histone-methylation processes. J Invest Dermatol. 2014;134:2192-2201 pubmed publisher
Gieseke F, Kruchen A, Tzaribachev N, Bentzien F, Dominici M, Müller I. Proinflammatory stimuli induce galectin-9 in human mesenchymal stromal cells to suppress T-cell proliferation. Eur J Immunol. 2013;43:2741-9 pubmed publisher
Willart M, Deswarte K, Pouliot P, Braun H, Beyaert R, Lambrecht B, et al. Interleukin-1? controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33. J Exp Med. 2012;209:1505-17 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Gudjonsson J, Johnston A, Stoll S, Riblett M, Xing X, Kochkodan J, et al. Evidence for altered Wnt signaling in psoriatic skin. J Invest Dermatol. 2010;130:1849-59 pubmed publisher
Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, et al. Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1{alpha}, and TNF-{alpha} Recapitulates Some Features of Psoriasis. J Immunol. 2010;184:5263-5270 pubmed publisher
Jung D, Che Z, Kim J, Kim K, Kim K, Williams D, et al. Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Int J Cancer. 2010;127:332-44 pubmed publisher
Gonzalez R, Seurynck Servoss S, Crowley S, Brown M, Omenn G, Hayes D, et al. Development and validation of sandwich ELISA microarrays with minimal assay interference. J Proteome Res. 2008;7:2406-14 pubmed publisher
Biancotto A, Iglehart S, Lisco A, Vanpouille C, Grivel J, Lurain N, et al. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res Hum Retroviruses. 2008;24:453-62 pubmed publisher
Pfleger C, Schloot N, ter Veld F. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J Immunol Methods. 2008;329:214-8 pubmed
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, et al. Ultrasensitive flow-based immunoassays using single-molecule counting. Clin Chem. 2007;53:1990-5 pubmed
Kelley M, Rose A, Song K, Chen Y, Bradley J, Rookhuizen D, et al. Synergism of TNF and IL-1 in the induction of matrix metalloproteinase-3 in trabecular meshwork. Invest Ophthalmol Vis Sci. 2007;48:2634-43 pubmed
Wilson H, Varcoe R, Stokes L, Holland K, Francis S, Dower S, et al. P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells. Br J Pharmacol. 2007;151:115-27 pubmed
Brechter A, Lerner U. Bradykinin potentiates cytokine-induced prostaglandin biosynthesis in osteoblasts by enhanced expression of cyclooxygenase 2, resulting in increased RANKL expression. Arthritis Rheum. 2007;56:910-23 pubmed
Biancotto A, Grivel J, Iglehart S, Vanpouille C, Lisco A, Sieg S, et al. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood. 2007;109:4272-9 pubmed
Gleissner C, Zastrow A, Klingenberg R, Kluger M, Konstandin M, Celik S, et al. IL-10 inhibits endothelium-dependent T cell costimulation by up-regulation of ILT3/4 in human vascular endothelial cells. Eur J Immunol. 2007;37:177-92 pubmed
Johnson L, Clasper S, Holt A, Lalor P, Baban D, Jackson D. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. J Exp Med. 2006;203:2763-77 pubmed
Abe Y, Fornage M, Yang C, Bui Thanh N, Wise V, Chen H, et al. L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion. Atherosclerosis. 2007;192:56-66 pubmed
Li Y, Fan J, Chen M, Li W, Woodley D. Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration. J Invest Dermatol. 2006;126:2096-105 pubmed
Palmer G, Trolliet S, Talabot Ayer D, Mezin F, Magne D, Gabay C. Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1). Cytokine. 2005;31:153-60 pubmed
Dulos J, van der Vleuten M, Kavelaars A, Heijnen C, Boots A. CYP7B expression and activity in fibroblast-like synoviocytes from patients with rheumatoid arthritis: regulation by proinflammatory cytokines. Arthritis Rheum. 2005;52:770-8 pubmed
Kao C, Chen Y, Thai P, Wachi S, Huang F, Kim C, et al. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways. J Immunol. 2004;173:3482-91 pubmed
Morita K, Miyasako T, Kitayama S, Dohi T. Interleukin-1 inhibits voltage-dependent P/Q-type Ca2+ channel associated with the inhibition of the rise of intracellular free Ca2+ concentration and catecholamine release in adrenal chromaffin cells. Biochim Biophys Acta. 2004;1673:160-9 pubmed
Kawano Y, Fukuda J, Itoh H, Takai N, Nasu K, Miyakawa I. The effect of inflammatory cytokines on secretion of macrophage colony-stimulating factor and monocyte chemoattractant protein-1 in human granulosa cells. Am J Reprod Immunol. 2004;52:124-8 pubmed
Smith D, Hanna R, Moore H, Chen H, Farese A, MacVittie T, et al. The soluble form of IL-1 receptor accessory protein enhances the ability of soluble type II IL-1 receptor to inhibit IL-1 action. Immunity. 2003;18:87-96 pubmed
Szabowski A, Maas Szabowski N, Andrecht S, Kolbus A, Schorpp Kistner M, Fusenig N, et al. c-Jun and JunB antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin. Cell. 2000;103:745-55 pubmed
product information
brand :
R&D Systems
master code :
200-LA
SKU :
200-LA-002
product name :
Recombinant Human IL-1 alpha/IL-1F1 Protein
unit size :
2 ug (also 10 ug)
seo description :
The Recombinant Human IL-1 alpha/IL-1F1 Protein from R&D Systems is derived from E. coli. The Recombinant Human IL-1 alpha/IL-1F1 Protein has been validated for the following applications: Bioactivity.
target :
IL-1 alpha/IL-1F1
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
dilution :
Bioactivity
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
species :
Human
theoretical molecular weight :
18 kDa
gene symbol :
IL1A
details of functionality :
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. etáal . (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. etáal . (eds): IRL Press. 272. The ED50 for this effect is 1-6 pg/mL.
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant Human IL-1 alpha/IL-1F1 Protein Bioactivity
accessionNumbers :
Q53QF9
applications :
Bioactivity
source :
E. coli-derived Recombinant Human IL-1 alpha/IL-1F1 Protein
USD :
207 USD
alt names :
BAF, Hematopoietin-1, IL1, IL1 alpha, IL-1 alpha, IL1A, IL-1A, IL1-ALPHA, IL1F1, IL-1F1, IL1F1hematopoietin-1, interleukin 1, alpha, interleukin-1 alpha, LAF, LEM, preinterleukin 1 alpha, pro-interleukin-1-alpha
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.