Recombinant Mouse CD47 Fc Chimera Protein, CF | R&D Systems 1866-CD-050 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse CD47 Fc Chimera Protein, CF

catalog :

1866-CD-050

quantity :

50 ug

price :

443 USD

citations: 4

Reference
Tang Z, Zhong M, Qian J, Galindo C, Davidson D, Li J, et al. CD47 masks pro-phagocytic ligands in cis on tumor cells to suppress antitumor immunity. Nat Immunol. 2023;24:2032-2041 pubmed publisher
Pei W, Wan X, Shahzad K, Zhang L, Song S, Jin X, et al. Direct modulation of myelin-autoreactive CD4+ and CD8+ T cells in EAE mice by a tolerogenic nanoparticle co-carrying myelin peptide-loaded major histocompatibility complexes, CD47 and multiple regulatory molecules. Int J Nanomedicine. 2018;13:3731-3750 pubmed publisher
Shahzad K, Wan X, Zhang L, Pei W, Zhang A, Younis M, et al. On-target and direct modulation of alloreactive T cells by a nanoparticle carrying MHC alloantigen, regulatory molecules and CD47 in a murine model of alloskin transplantation. Drug Deliv. 2018;25:703-715 pubmed publisher
Qie Y, Yuan H, von Roemeling C, Chen Y, Liu X, Shih K, et al. Surface modification of nanoparticles enables selective evasion of phagocytic clearance by distinct macrophage phenotypes. Sci Rep. 2016;6:26269 pubmed publisher

product information

master code :

1866-CD

SKU :

1866-CD-050

product name :

Recombinant Mouse CD47 Fc Chimera Protein, CF

unit size :

50 ug

description :

The Recombinant Mouse CD47 Fc Chimera Protein, CF from R&D Systems is derived from NS0. The Recombinant Mouse CD47 Fc Chimera Protein, CF has been validated for the following applications: Bioactivity.

target :

CD47

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Mouse

observed molecular weight :

60-70 kDa, reducing conditions

theoretical molecular weight :

42.3 kDa (monomer)

gene symbol :

Cd47

details of functionality :

Measured by its binding ability in a functional ELISA. When Recombinant Mouse SIRP alpha /CD172a Fc Chimera (Catalog # 7154-SA ) is coated at 2 ╡g/mL, Recombinant Mouse CD47 Fc Chimera binds with an apparent Kd 0.6 nM. Also measured by its ability to antagonize mouse red blood cell adhesion to immobilized Recombinant Mouse SIRP alpha /CD172a Fc Chimera (Catalog # 7154-SA ). The ED50 for this effect is 2.0-8.0 ╡g/mL. Optimal dilutions should be determined by each laboratory for each application.

endotoxin note :

<0.01 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_034711

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived mouse CD47 protein Mouse CD47 (Gln19-Pro158) Accession # NP_034711 IEGRMD Human IgG1 (Pro100-Lys330) N-terminus C-terminus

source short :

NS0

USD :

443 USD

alt names :

antigen identified by monoclonal 1D8, Antigenic surface determinant protein OA3, CD47, CD47 antigen, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, CD47 molecule, IAP, IAPintegrin associated protein, Integrin-associated protein, leukocyte surface antigen CD47, MER6, MER6integrin-associated signal transducer, OA3, Protein MER6, Rh-related antigen

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.