product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Mouse Granzyme B Protein, CF
catalog :
1865-SE-010
quantity :
10 ug
price :
432 USD
more info or order :
R&D Systems product webpage
citations: 1
Reference
Matthiesen S, Zaeck L, Franzke K, Jahnke R, Fricke C, Mauermeir M, et al. Coxiella burnetii-Infected NK Cells Release Infectious Bacteria by Degranulation. Infect Immun. 2020;88: pubmed publisher
product information
master code :
1865-SE
SKU :
1865-SE-010
product name :
Recombinant Mouse Granzyme B Protein, CF
unit size :
10 ug
description :
The Recombinant Mouse Granzyme B Protein, CF from R&D Systems is derived from NS0. The Recombinant Mouse Granzyme B Protein, CF has been validated for the following applications: Enzyme Activity.
target :
Granzyme B
category :
Proteins and Enzymes
buffer :
Supplied as a 0.2 ╡m filtered solution in HEPES, NaCl and CaCl2.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Mouse
observed molecular weight :
Multiple bands between 34-38 kDa, reducing conditions
theoretical molecular weight :
27 kDa
gene symbol :
Gzmb
details of functionality :
Measured by its ability to cleave a peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5Æ-Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al . (1999) J. Biol. Chem. 274 :30468. The specific activity is 2,000 pmol/min/╡g as measured under the described conditions.
endotoxin note :
<1.0 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
P04187
applications :
Enzyme Activity
source long :
Mouse myeloma cell line, NS0-derived mouse Granzyme B protein Lys17-Ser247, with a C-terminal 10-His tag
source short :
NS0
USD :
432 USD
alt names :
C11, CCPI, CGL1, CGL-1, CSPB, CSP-B, CTLA1, CTLA-1, CTSGL1, Fragmentin-2, granzyme B, Granzyme-2, GranzymeB, GRB, GrzB, GZMB, HLP, SECT
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.