This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus
catalog :
1845-SH
quantity :
25 ug (also 100 ug, 500 ug)
price :
299 USD
citations: 38
Reference
Yang C, Yong L, Liang C, Li Y, Ma Y, Wei F, et al. Genetic landscape and ligand-dependent activation of sonic hedgehog-Gli1 signaling in chordomas: a novel therapeutic target. Oncogene. 2020;39:4711-4727 pubmed publisher
Feng W, Wilkins O, Bang S, Ung M, Li J, An J, et al. CD36-Mediated Metabolic Rewiring of Breast Cancer Cells Promotes Resistance to HER2-Targeted Therapies. Cell Rep. 2019;29:3405-3420.e5 pubmed publisher
Ferent J, Constable S, Gigante E, Yam P, Mariani L, Legue E, et al. The Ciliary Protein Arl13b Functions Outside of the Primary Cilium in Shh-Mediated Axon Guidance. Cell Rep. 2019;29:3356-3366.e3 pubmed publisher
White C, Jayasekara W, Picard D, Chen J, Watkins D, Cain J, et al. A Sexually Dimorphic Role for STAT3 in Sonic Hedgehog Medulloblastoma. Cancers (Basel). 2019;11: pubmed publisher
Zanin J, Verpeut J, Li Y, Shiflett M, Wang S, Santhakumar V, et al. The p75NTR Influences Cerebellar Circuit Development and Adult Behavior via Regulation of Cell Cycle Duration of Granule Cell Progenitors. J Neurosci. 2019;39:9119-9129 pubmed publisher
Ma L, Wang Y, Hui Y, Du Y, Chen Z, Feng H, et al. WNT/NOTCH Pathway Is Essential for the Maintenance and Expansion of Human MGE Progenitors. Stem Cell Reports. 2019;12:934-949 pubmed publisher
Riaz S, Ke Y, Wang F, Kayani M, Malik M. Influence of SHH/GLI1 axis on EMT mediated migration and invasion of breast cancer cells. Sci Rep. 2019;9:6620 pubmed publisher
Elkouris M, Kouroupi G, Vourvoukelis A, Papagiannakis N, Kaltezioti V, Matsas R, et al. Long Non-coding RNAs Associated With Neurodegeneration-Linked Genes Are Reduced in Parkinson's Disease Patients. Front Cell Neurosci. 2019;13:58 pubmed publisher
Ooi J, Langley S, Xu X, Utami K, Sim B, Huang Y, et al. Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects. Cell Rep. 2019;26:2494-2508.e7 pubmed publisher
Lang C, Campbell K, Ryan B, Carling P, Attar M, Vowles J, et al. Single-Cell Sequencing of iPSC-Dopamine Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes. Cell Stem Cell. 2019;24:93-106.e6 pubmed publisher
Tan K, Lim W, Chai C, Kukumberg M, Lim K, Goh E, et al. Sequential Application of Discrete Topographical Patterns Enhances Derivation of Functional Mesencephalic Dopaminergic Neurons from Human Induced Pluripotent Stem Cells. Sci Rep. 2018;8:9567 pubmed publisher
Xu C, Zou C, Hussain M, Shi W, Shao Y, Jiang Z, et al. High expression of Sonic hedgehog in allergic airway epithelia contributes to goblet cell metaplasia. Mucosal Immunol. 2018;11:1306-1315 pubmed publisher
Hu X, Lee J, Zheng X, Zhang J, Lin X, Song Y, et al. Efficient induction of functional ameloblasts from human keratinocyte stem cells. Stem Cell Res Ther. 2018;9:126 pubmed publisher
Kim J, Lee J, Hwang H, Lee D, Park C, Jung S, et al. Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells. Exp Mol Med. 2018;50:24 pubmed publisher
Chen C, Breslin M, Lan M. Sonic hedgehog signaling pathway promotes INSM1 transcription factor in neuroendocrine lung cancer. Cell Signal. 2018;46:83-91 pubmed publisher
Ye X, Hong W, Hao B, Peng G, Huang L, Zhao Z, et al. PM2.5 promotes human bronchial smooth muscle cell migration via the sonic hedgehog signaling pathway. Respir Res. 2018;19:37 pubmed publisher
Shall G, Menosky M, Decker S, Nethala P, Welchko R, Lévêque X, et al. Effects of Passage Number and Differentiation Protocol on the Generation of Dopaminergic Neurons from Rat Bone Marrow-Derived Mesenchymal Stem Cells. Int J Mol Sci. 2018;19: pubmed publisher
Fink D, Sun M, Heyne G, Everson J, Chung H, Park S, et al. Coordinated d-cyclin/Foxd1 activation drives mitogenic activity of the Sonic Hedgehog signaling pathway. Cell Signal. 2018;44:1-9 pubmed publisher
Xie S, Li G, Han C, Yu Y, Li N. RKIP reduction enhances radioresistance by activating the Shh signaling pathway in non-small-cell lung cancer. Onco Targets Ther. 2017;10:5605-5619 pubmed publisher
Kasiri S, Shao C, Chen B, Wilson A, Yenerall P, Timmons B, et al. GLI1 Blockade Potentiates the Antitumor Activity of PI3K Antagonists in Lung Squamous Cell Carcinoma. Cancer Res. 2017;77:4448-4459 pubmed publisher
Bergen D, Stevenson N, Skinner R, Stephens D, Hammond C. The Golgi matrix protein giantin is required for normal cilia function in zebrafish. Biol Open. 2017;6:1180-1189 pubmed publisher
Spicer L, Schütz L, Williams J, Schreiber N, Evans J, Totty M, et al. G protein-coupled receptor 34 in ovarian granulosa cells of cattle: changes during follicular development and potential functional implications. Domest Anim Endocrinol. 2017;59:90-99 pubmed publisher
Chanda D, Kurundkar A, Rangarajan S, Locy M, Bernard K, Sharma N, et al. Developmental Reprogramming in Mesenchymal Stromal Cells of Human Subjects with Idiopathic Pulmonary Fibrosis. Sci Rep. 2016;6:37445 pubmed publisher
Wang X, Wei S, Zhao Y, Shi C, Liu P, Zhang C, et al. Anti-proliferation of breast cancer cells with itraconazole: Hedgehog pathway inhibition induces apoptosis and autophagic cell death. Cancer Lett. 2017;385:128-136 pubmed publisher
Bora Singhal N, Perumal D, Nguyen J, Chellappan S. Gli1-Mediated Regulation of Sox2 Facilitates Self-Renewal of Stem-Like Cells and Confers Resistance to EGFR Inhibitors in Non-Small Cell Lung Cancer. Neoplasia. 2015;17:538-51 pubmed publisher
Matevossian A, Resh M. Hedgehog Acyltransferase as a target in estrogen receptor positive, HER2 amplified, and tamoxifen resistant breast cancer cells. Mol Cancer. 2015;14:72 pubmed publisher
Belgacem Y, Borodinsky L. Inversion of Sonic hedgehog action on its canonical pathway by electrical activity. Proc Natl Acad Sci U S A. 2015;112:4140-5 pubmed publisher
Tang C, Pan Y, Luo H, Xiong W, Zhu H, Ruan H, et al. Hedgehog signaling stimulates the conversion of cholesterol to steroids. Cell Signal. 2015;27:487-97 pubmed publisher
Hartfield E, Yamasaki Mann M, Ribeiro Fernandes H, Vowles J, James W, Cowley S, et al. Physiological characterisation of human iPS-derived dopaminergic neurons. PLoS ONE. 2014;9:e87388 pubmed publisher
Williams E, Zhong X, Mohamed A, Li R, Liu Y, Dong Q, et al. Mutant astrocytes differentiated from Rett syndrome patients-specific iPSCs have adverse effects on wild-type neurons. Hum Mol Genet. 2014;23:2968-80 pubmed publisher
Saito A, Kanemoto S, Zhang Y, Asada R, Hino K, Imaizumi K. Chondrocyte proliferation regulated by secreted luminal domain of ER stress transducer BBF2H7/CREB3L2. Mol Cell. 2014;53:127-39 pubmed publisher
Chen D, Persson A, Sun Y, Salford L, Nord D, Englund E, et al. Better prognosis of patients with glioma expressing FGF2-dependent PDGFRA irrespective of morphological diagnosis. PLoS ONE. 2013;8:e61556 pubmed publisher
Liu Y, Weick J, Liu H, Krencik R, Zhang X, Ma L, et al. Medial ganglionic eminence-like cells derived from human embryonic stem cells correct learning and memory deficits. Nat Biotechnol. 2013;31:440-7 pubmed publisher
Cai J, Schleidt S, Pelta Heller J, Hutchings D, Cannarsa G, Iacovitti L. BMP and TGF-β pathway mediators are critical upstream regulators of Wnt signaling during midbrain dopamine differentiation in human pluripotent stem cells. Dev Biol. 2013;376:62-73 pubmed publisher
Kirkeby A, Nelander J, Parmar M. Generating regionalized neuronal cells from pluripotency, a step-by-step protocol. Front Cell Neurosci. 2012;6:64 pubmed publisher
Strand M, Wilson S, Dembinski J, Holsworth D, Khvat A, Okun I, et al. A novel synthetic smoothened antagonist transiently inhibits pancreatic adenocarcinoma xenografts in a mouse model. PLoS ONE. 2011;6:e19904 pubmed publisher
Fabre P, Shimogori T, Charron F. Segregation of ipsilateral retinal ganglion cell axons at the optic chiasm requires the Shh receptor Boc. J Neurosci. 2010;30:266-75 pubmed publisher
Kiprilov E, Awan A, Desprat R, Velho M, Clement C, Byskov A, et al. Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery. J Cell Biol. 2008;180:897-904 pubmed publisher
product information
brand :
R&D Systems
master code :
1845-SH
SKU :
1845-SH-025
product name :
Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus
description :
The Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus from R&D Systems is derived from E. coli. The Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus has been validated for the following applications: Bioactivity.
target :
Sonic Hedgehog/Shh
category :
Proteins and Enzymes
unit size :
25 ug (also 100 ug, 500 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS and NaCl with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
observed molecular weight :
22 kDa, reducing conditions
theoretical molecular weight :
20 kDa
gene symbol :
SHH
details of functionality :
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al . (1997) Biochem. Biophys. Res. Commun. 237 :465. The ED50 for this effect is 0.1-0.4 µg/mL.
endotoxin note :
<0.01 EU per 1 µg of the protein by the LAL method.
catalog number base :
1845-SH
accessionNumbers :
NP_000184
applications :
Bioactivity
source :
E. coli -derived human Sonic Hedgehog/Shh protein Cys24-Gly197 (Cys24Ile-Ile), with an N-terminal Met
extended description :
A New rh Shh is Available! C-term cholesterol, N-term fatty acid-modified; 14-fold better activity!
2020 USD :
299
2021 USD :
299 USD
product details :
A New rh Shh is Available! C-term cholesterol, N-term fatty acid-modified; 14-fold better activity!
alt names :
HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.