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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus, CF
catalog :
1845-SH-025/CF
quantity :
25 ug (also 100 ug)
price :
355 USD
more info or order :
citations: 58
Reference
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Riaz S, Ke Y, Wang F, Kayani M, Malik M. Influence of SHH/GLI1 axis on EMT mediated migration and invasion of breast cancer cells. Sci Rep. 2019;9:6620 pubmed publisher
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Chen C, Breslin M, Lan M. Sonic hedgehog signaling pathway promotes INSM1 transcription factor in neuroendocrine lung cancer. Cell Signal. 2018;46:83-91 pubmed publisher
Ye X, Hong W, Hao B, Peng G, Huang L, Zhao Z, et al. PM2.5 promotes human bronchial smooth muscle cell migration via the sonic hedgehog signaling pathway. Respir Res. 2018;19:37 pubmed publisher
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product information
master code :
1845-SH/CF
SKU :
1845-SH-025/CF
product name :
Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus, CF
unit size :
25 ug (also 100 ug)
description :
The Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus, CF from R&D Systems is derived from E. coli. The Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus, CF has been validated for the following applications: Bioactivity.
target :
Sonic Hedgehog/Shh
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS and NaCl.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Human
observed molecular weight :
22 kDa, reducing conditions
theoretical molecular weight :
20 kDa
gene symbol :
SHH
details of functionality :
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al . (1997) Biochem. Biophys. Res. Commun. 237 :465. The ED50 for this effect is 0.1-0.4 ╡g/mL.
endotoxin note :
<0.01 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus Bioactivity
accessionNumbers :
NP_000184.1
applications :
Bioactivity
source long :
E. coli -derived human Sonic Hedgehog/Shh protein Cys24-Gly197 (Cys24Ile-Ile), with an N-terminal Met
source short :
E. coli
USD :
355 USD
alt names :
HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.