Recombinant Human sFRP-4 Protein, CF | R&D Systems 1827-SF-025/CF product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human sFRP-4 Protein, CF

catalog :

1827-SF-025/CF

quantity :

25 ug

price :

400 USD

more info or order :

R&D Systems product webpage

citations: 9

Reference
Gay D, Ghinatti G, Guerrero Juarez C, Ferrer R, Ferri F, Lim C, et al. Phagocytosis of Wnt inhibitor SFRP4 by late wound macrophages drives chronic Wnt activity for fibrotic skin healing. Sci Adv. 2020;6:eaay3704 pubmed publisher
Deshmukh A, Arfuso F, Newsholme P, Dharmarajan A. Epigenetic demethylation of sFRPs, with emphasis on sFRP4 activation, leading to Wnt signalling suppression and histone modifications in breast, prostate, and ovary cancer stem cells. Int J Biochem Cell Biol. 2019;109:23-32 pubmed publisher
Deshmukh A, Arfuso F, Newsholme P, Dharmarajan A. Regulation of Cancer Stem Cell Metabolism by Secreted Frizzled-Related Protein 4 (sFRP4). Cancers (Basel). 2018;10: pubmed publisher
Batsali A, Pontikoglou C, Koutroulakis D, Pavlaki K, Damianaki A, Mavroudi I, et al. Differential expression of cell cycle and WNT pathway-related genes accounts for differences in the growth and differentiation potential of Wharton's jelly and bone marrow-derived mesenchymal stem cells. Stem Cell Res Ther. 2017;8:102 pubmed publisher
Marthandan S, Baumgart M, Priebe S, Groth M, Schaer J, Kaether C, et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS ONE. 2016;11:e0154531 pubmed publisher
Bai J, Liu Z, Xu Z, Ke F, Zhang L, Zhu H, et al. Epigenetic downregulation of SFRP4 contributes to epidermal hyperplasia in psoriasis. J Immunol. 2015;194:4185-98 pubmed publisher
Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, et al. Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways. PLoS ONE. 2014;9:e104937 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Wawrzak D, Metioui M, Willems E, Hendrickx M, De Genst E, Leyns L. Wnt3a binds to several sFRPs in the nanomolar range. Biochem Biophys Res Commun. 2007;357:1119-23 pubmed

product information

brand :

R&D Systems

master code :

1827-SF/CF

SKU :

1827-SF-025/CF

product name :

Recombinant Human sFRP-4 Protein, CF

unit size :

25 ug

seo description :

The Recombinant Human sFRP-4 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human sFRP-4 Protein, CF has been validated for the following applications: Bioactivity.

target :

sFRP-4

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

dilution :

Bioactivity

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

observed molecular weight :

55 kDa, reducing conditions

theoretical molecular weight :

38.9 kDa

gene symbol :

SFRP4

details of functionality :

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 4-20 ╡g/mL, in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN ). Optimal dilutions should be determined by each laboratory for each application.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_003005

applications :

Bioactivity

source :

NS0-derived Recombinant Human sFRP-4 Protein, CF

USD :

400 USD

alt names :

DDC-4, Frizzled protein, human endometrium, FRP-4, FRP-AP, FrpHE, FRPHEMGC26498, FrzB2, secreted frizzled-related protein 4, secreted frizzled-related protein 4; secreted frizzled-related protein 4, sFRP4, sFRP-4

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage