Recombinant Equine TNF-alpha Protein | R&D Systems 1814-ET-025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Equine TNF-alpha Protein

catalog :

1814-ET-025

quantity :

25 ug

price :

432 USD

citations: 8

Reference
Moyo N, Westcott D, Simmonds R, Steinbach F. Equine Arteritis Virus in Monocytic Cells Suppresses Differentiation and Function of Dendritic Cells. Viruses. 2023;15: pubmed publisher
Cvitaš I, Oberhaensli S, Leeb T, Marti E. Equine keratinocytes in the pathogenesis of insect bite hypersensitivity: Just another brick in the wall?. PLoS ONE. 2022;17:e0266263 pubmed publisher
Jeremiasse B, Matta C, Fellows C, Boocock D, Smith J, Liddell S, et al. Alterations in the chondrocyte surfaceome in response to pro-inflammatory cytokines. BMC Mol Cell Biol. 2020;21:47 pubmed publisher
Lange Consiglio A, Romele P, Magatti M, Silini A, Idda A, Martino N, et al. Priming with inflammatory cytokines is not a prerequisite to increase immune-suppressive effects and responsiveness of equine amniotic mesenchymal stromal cells. Stem Cell Res Ther. 2020;11:99 pubmed publisher
Martin L, Johnson P, Amorim J, DeClue A. Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study. Int J Mol Sci. 2020;21: pubmed publisher
Barrachina L, Cequier A, Romero A, Vitoria A, Zaragoza P, Vázquez F, et al. Allo-antibody production after intraarticular administration of mesenchymal stem cells (MSCs) in an equine osteoarthritis model: effect of repeated administration, MSC inflammatory stimulation, and equine leukocyte antigen (ELA) compatibility. Stem Cell Res Ther. 2020;11:52 pubmed publisher
Berghaus L, Giguere S, Bordin A, Cohen N. Effects of priming with cytokines on intracellular survival and replication of Rhodococcus equi in equine macrophages. Cytokine. 2018;102:7-11 pubmed publisher
Huang H, Lavoie Lamoureux A, Moran K, Lavoie J. IL-4 stimulates the expression of CXCL-8, E-selectin, VEGF, and inducible nitric oxide synthase mRNA by equine pulmonary artery endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2007;292:L1147-54 pubmed

product information

master code :

1814-ET

SKU :

1814-ET-025

product name :

Recombinant Equine TNF-alpha Protein

unit size :

25 ug

description :

The Recombinant Equine TNF-alpha Protein from R&D Systems is derived from E. coli. The Recombinant Equine TNF-alpha Protein has been validated for the following applications: Bioactivity.

target :

TNF-alpha

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Equine

theoretical molecular weight :

17.2 kDa

gene symbol :

TNF

details of functionality :

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycináD. Matthews,áN. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 221. The ED50 for this effect is 0.025-0.1 ng/mL.

endotoxin note :

<0.01 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_001075288

applications :

Bioactivity

source long :

E. coli -derived equine TNF-alpha protein Leu78-Leu234, with an N-terminal Met

source short :

E. coli

USD :

432 USD

alt names :

APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.