Recombinant Human MD-2 Protein, CF | R&D Systems 1787-MD-050/CF product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human MD-2 Protein, CF

catalog :

1787-MD-050/CF

quantity :

50 ug

price :

400 USD

citations: 17

Reference
Zhang J, Sun Y, Sun C, Shang D. The antimicrobial peptide LK2(6)A(L) exhibits anti-inflammatory activity by binding to the myeloid differentiation 2 domain and protects against LPS-induced acute lung injury in mice. Bioorg Chem. 2023;132:106376 pubmed publisher
Jung F, Staltner R, Baumann A, Burger K, Halilbasic E, Hellerbrand C, et al. A Xanthohumol-Rich Hop Extract Diminishes Endotoxin-Induced Activation of TLR4 Signaling in Human Peripheral Blood Mononuclear Cells: A Study in Healthy Women. Int J Mol Sci. 2022;23: pubmed publisher
Luo W, Yang L, Qian C, Ma B, Manjengwa G, Miao X, et al. Flavokawain B alleviates LPS-induced acute lung injury via targeting myeloid differentiation factor 2. Acta Pharmacol Sin. 2022;43:1758-1768 pubmed publisher
Zhu W, Sahar N, Javaid H, Pak E, Liang G, Wang Y, et al. Exercise-Induced Irisin Decreases Inflammation and Improves NAFLD by Competitive Binding with MD2. Cells. 2021;10: pubmed publisher
Puck A, K xfc nig S, Modak M, May L, Fritz P, Battin C, et al. The soluble cytoplasmic tail of CD45 regulates T-cell activation via TLR4 signaling. Eur J Immunol. 2021;51:3176-3185 pubmed publisher
Sun S, Yan Z, Shui X, Qi W, Chen Y, Xu X, et al. Astilbin prevents osteoarthritis development through the TLR4/MD-2 pathway. J Cell Mol Med. 2020;24:13104-13114 pubmed publisher
Wang Y, Luo W, Han J, Khan Z, Fang Q, Jin Y, et al. MD2 activation by direct AGE interaction drives inflammatory diabetic cardiomyopathy. Nat Commun. 2020;11:2148 pubmed publisher
Chen T, Huang W, Qian J, Luo W, Shan P, Cai Y, et al. Macrophage-derived myeloid differentiation protein 2 plays an essential role in ox-LDL-induced inflammation and atherosclerosis. EBioMedicine. 2020;53:102706 pubmed publisher
Shang P, Tang Q, Hu Z, Huang S, Hu Y, Zhu J, et al. Procyanidin B3 alleviates intervertebral disc degeneration via interaction with the TLR4/MD-2 complex. J Cell Mol Med. 2020;24:3701-3711 pubmed publisher
Ito T, Yamamoto S, Yamaguchi K, Sato M, Kaneko Y, Goto S, et al. Inorganic polyphosphate potentiates lipopolysaccharide-induced macrophage inflammatory response. J Biol Chem. 2020;295:4014-4023 pubmed publisher
Sun S, He M, Wang Y, Yang H, Al Abed Y. Folic acid derived-P5779 mimetics regulate DAMP-mediated inflammation through disruption of HMGB1:TLR4:MD-2 axes. PLoS ONE. 2018;13:e0193028 pubmed publisher
S Lashkari B, Anumba D. Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4. PLoS ONE. 2017;12:e0173646 pubmed publisher
Park S, Baek S, Yun J, Lee S, Yoon D, Jung J, et al. IRAK4 as a molecular target in the amelioration of innate immunity-related endotoxic shock and acute liver injury by chlorogenic acid. J Immunol. 2015;194:1122-30 pubmed publisher
Bozza S, Campo S, Arseni B, Inforzato A, Ragnar L, Bottazzi B, et al. PTX3 binds MD-2 and promotes TRIF-dependent immune protection in aspergillosis. J Immunol. 2014;193:2340-8 pubmed publisher
Chen Y, Shiau A, Wang S, Yang J, Chang S, Wu C, et al. Zhankuic acid A isolated from Taiwanofungus camphoratus is a novel selective TLR4/MD-2 antagonist with anti-inflammatory properties. J Immunol. 2014;192:2778-86 pubmed publisher
Feng C, Stamatos N, Dragan A, Medvedev A, Whitford M, Zhang L, et al. Sialyl residues modulate LPS-mediated signaling through the Toll-like receptor 4 complex. PLoS ONE. 2012;7:e32359 pubmed publisher
Macagno A, Molteni M, Rinaldi A, Bertoni F, Lanzavecchia A, Rossetti C, et al. A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression. J Exp Med. 2006;203:1481-92 pubmed

product information

master code :

1787-MD/CF

SKU :

1787-MD-050/CF

product name :

Recombinant Human MD-2 Protein, CF

unit size :

50 ug

description :

The Recombinant Human MD-2 Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human MD-2 Protein, CF has been validated for the following applications: Binding Activity.

target :

MD2

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in Acetonitrile and TFA.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

theoretical molecular weight :

19.2 kDa (monomer)

gene symbol :

LY96

details of functionality :

Measured by its binding ability in a functional ELISA. When recombinant human TLR-4 is Immobilized at 2 ╡g/mL (100 ╡L/well), the concentration of rhMD-2 that produces 50% optimal binding response is found to be approximately 0.03-0.15 ╡g/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

BAA78717

applications :

Binding Activity

source long :

E. coli -derived human MD2 protein Met Human MD-2 (Glu17 - Asn160) Accession # BAA78717 IEGRGGGSGGGSGGGS 10-His tag N-terminus C-terminus

source short :

E. coli

USD :

400 USD

alt names :

ESOP1, ESOP-1, Ly-96, LY96, lymphocyte antigen 96, MD2, MD-2, myeloid differentiation protein-2, Protein MD-2

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after opening. 3 months, -20 to -70 ░C under sterile conditions after opening.