Recombinant Mouse Dkk-1 (Histidine-tagged) Protein, CF | R&D Systems 1765-DK-010/CF product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse Dkk-1 (Histidine-tagged) Protein, CF

catalog :

1765-DK-010/CF

quantity :

10 ug

price :

432 USD

citations: 8

Reference
Yachie Kinoshita A, Onishi K, Ostblom J, Langley M, Pósfai E, Rossant J, et al. Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions. Mol Syst Biol. 2018;14:e7952 pubmed publisher
Trowbridge J, Guezguez B, Moon R, Bhatia M. Wnt3a activates dormant c-Kit(-) bone marrow-derived cells with short-term multilineage hematopoietic reconstitution capacity. Stem Cells. 2010;28:1379-89 pubmed publisher
Hansson M, Olesen D, Peterslund J, Engberg N, Kahn M, Winzi M, et al. A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. Dev Biol. 2009;330:286-304 pubmed publisher
Nakanishi M, Kurisaki A, Hayashi Y, Warashina M, Ishiura S, Kusuda Furue M, et al. Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium. FASEB J. 2009;23:114-22 pubmed publisher
Chen Y, Hu Y, Lu K, Flannery J, Ma J. Very low density lipoprotein receptor, a negative regulator of the wnt signaling pathway and choroidal neovascularization. J Biol Chem. 2007;282:34420-8 pubmed
Kwon C, Arnold J, Hsiao E, Taketo M, Conklin B, Srivastava D. Canonical Wnt signaling is a positive regulator of mammalian cardiac progenitors. Proc Natl Acad Sci U S A. 2007;104:10894-9 pubmed
Wawrzak D, Metioui M, Willems E, Hendrickx M, De Genst E, Leyns L. Wnt3a binds to several sFRPs in the nanomolar range. Biochem Biophys Res Commun. 2007;357:1119-23 pubmed
Lindsley R, Gill J, Kyba M, Murphy T, Murphy K. Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development. 2006;133:3787-96 pubmed

product information

master code :

1765-DK/CF

SKU :

1765-DK-010/CF

product name :

Recombinant Mouse Dkk-1 (Histidine-tagged) Protein, CF

unit size :

10 ug

description :

The Recombinant Mouse Dkk-1 (Histidine-tagged) Protein, CF from R&D Systems is derived from NS0. The Recombinant Mouse Dkk-1 (Histidine-tagged) Protein, CF has been validated for the following applications: Bioactivity.

target :

Dkk-1

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Mouse

observed molecular weight :

38 kDa and 41 kDa, reducing conditions

theoretical molecular weight :

27.6 kDa

gene symbol :

Dkk1

details of functionality :

Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Dkk-1 inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN ). The ED50 for this effect is 30-180 ng/mL.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

top caption :

Recombinant Mouse Dkk-1 (Histidine-tagged) Protein Bioactivity

accessionNumbers :

O54908

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived mouse Dkk-1 protein Ser30-His272, with a C-terminal 10-His tag

source short :

NS0

extended description :

A New and Improved rm Dkk-1 is Available! It has a lower endotoxin specification, ~5 fold better activity, and no tag!

USD :

432 USD

product details :

A New and Improved rm Dkk-1 is Available! It has a lower endotoxin specification, ~5 fold better activity, and no tag!

alt names :

dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1 (Xenopus laevis), dickkopf related protein-1, Dickkopf-1, dickkopf-related protein 1, Dkk1, DKK-1, hDkk-1, SKdickkopf-1 like

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.