product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Mouse Dkk-1 (Histidine-tagged) Protein, CF
catalog :
1765-DK-010/CF
quantity :
10 ug
price :
432 USD
more info or order :
R&D Systems product webpage
citations: 8
Reference
Yachie Kinoshita A, Onishi K, Ostblom J, Langley M, Pósfai E, Rossant J, et al. Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions. Mol Syst Biol. 2018;14:e7952 pubmed publisher
Trowbridge J, Guezguez B, Moon R, Bhatia M. Wnt3a activates dormant c-Kit(-) bone marrow-derived cells with short-term multilineage hematopoietic reconstitution capacity. Stem Cells. 2010;28:1379-89 pubmed publisher
Hansson M, Olesen D, Peterslund J, Engberg N, Kahn M, Winzi M, et al. A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. Dev Biol. 2009;330:286-304 pubmed publisher
Nakanishi M, Kurisaki A, Hayashi Y, Warashina M, Ishiura S, Kusuda Furue M, et al. Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium. FASEB J. 2009;23:114-22 pubmed publisher
Chen Y, Hu Y, Lu K, Flannery J, Ma J. Very low density lipoprotein receptor, a negative regulator of the wnt signaling pathway and choroidal neovascularization. J Biol Chem. 2007;282:34420-8 pubmed
Kwon C, Arnold J, Hsiao E, Taketo M, Conklin B, Srivastava D. Canonical Wnt signaling is a positive regulator of mammalian cardiac progenitors. Proc Natl Acad Sci U S A. 2007;104:10894-9 pubmed
Wawrzak D, Metioui M, Willems E, Hendrickx M, De Genst E, Leyns L. Wnt3a binds to several sFRPs in the nanomolar range. Biochem Biophys Res Commun. 2007;357:1119-23 pubmed
Lindsley R, Gill J, Kyba M, Murphy T, Murphy K. Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development. 2006;133:3787-96 pubmed
product information
brand :
R&D Systems
master code :
1765-DK/CF
SKU :
1765-DK-010/CF
product name :
Recombinant Mouse Dkk-1 (Histidine-tagged) Protein, CF
unit size :
10 ug
seo description :
The Recombinant Mouse Dkk-1 (Histidine-tagged) Protein, CF from R&D Systems is derived from NS0. The Recombinant Mouse Dkk-1 (Histidine-tagged) Protein, CF has been validated for the following applications: Bioactivity.
target :
Dkk-1
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS with Trehalose.
conjugate :
Unconjugated
dilution :
Bioactivity
purity :
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Mouse
observed molecular weight :
38 kDa and 41 kDa, reducing conditions
theoretical molecular weight :
27.6 kDa
gene symbol :
Dkk1
details of functionality :
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Dkk-1 inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN ). The ED50 for this effect is 30-180 ng/mL.
endotoxin note :
<1.0 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant Mouse Dkk-1 (Histidine-tagged) Protein Bioactivity
accessionNumbers :
O54908
applications :
Bioactivity
source :
NS0-derived Recombinant Mouse Dkk-1 (Histidine-tagged) Protein, CF
extended description :
A New and Improved rm Dkk-1 is Available! It has a lower endotoxin specification, ~5 fold better activity, and no tag!
USD :
432 USD
product details :
A New and Improved rm Dkk-1 is Available! It has a lower endotoxin specification, ~5 fold better activity, and no tag!
alt names :
dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1 (Xenopus laevis), dickkopf related protein-1, Dickkopf-1, dickkopf-related protein 1, Dkk1, DKK-1, hDkk-1, SKdickkopf-1 like
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.