Recombinant Porcine IFN-alpha (Mammalian) Protein | R&D Systems 17105-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Porcine IFN-alpha (Mammalian) Protein

catalog :

17105-1

quantity :

100000 UN

price :

556 USD

citations: 8

Reference
Liu H, Zhao Y, Du H, Hao P, Tian H, Wang K, et al. IL-10 upregulates SOCS3 to inhibit type I interferon signaling to promote PoRVA replication in intestinal epithelial cells. Vet Microbiol. 2024;298:110259 pubmed publisher
Ba C, Tian C, Bo X, Xu C, Zhang Y, Sun X, et al. Porcine HERC6 acts as major E3 ligase for ISGylation and is auto-ISGylated for effective ISGylation in porcine. Microb Pathog. 2024;190:106633 pubmed publisher
Wang Z, Chen J, Zhang Q, Huang K, Ma D, Du Q, et al. Porcine circovirus type 2 infection inhibits the activation of type I interferon signaling via capsid protein and host gC1qR. Vet Microbiol. 2022;266:109354 pubmed publisher
Liu H, Shi B, Zhang Z, Zhao B, Zhao G, Li Y, et al. PRRSV Vaccine Strain-Induced Secretion of Extracellular ISG15 Stimulates Porcine Alveolar Macrophage Antiviral Response against PRRSV. Viruses. 2020;12: pubmed publisher
Su J, Zhang X, He B, Ge X, Han J, Zhou L, et al. Identification of three site mutations in nonstructural protein 1β, glycoprotein 3 and glycoprotein 5 that correlate with increased interferon α resistance of porcine reproductive and respiratory syndrome virus. Vet Microbiol. 2019;236:108395 pubmed publisher
Li Y, Darwich L, Mateu E. Characterization of the attachment and infection by Porcine reproductive and respiratory syndrome virus 1 isolates in bone marrow-derived dendritic cells. Vet Microbiol. 2018;223:181-188 pubmed publisher
Sang Y, Brichalli W, Rowland R, Blecha F. Genome-wide analysis of antiviral signature genes in porcine macrophages at different activation statuses. PLoS ONE. 2014;9:e87613 pubmed publisher
Miller L, Zanella E, Waters W, Lager K. Cytokine protein expression levels in tracheobronchial lymph node homogenates of pigs infected with pseudorabies virus. Clin Vaccine Immunol. 2010;17:728-34 pubmed publisher

product information

master code :

17105-1

SKU :

17105-1

product name :

Recombinant Porcine IFN-alpha (Mammalian) Protein

unit size :

100000 UN

description :

The Recombinant Porcine IFN-alpha (Mammalian) Protein has been validated for the following applications: Bioactivity.

target :

IFN-alpha

category :

Proteins and Enzymes

buffer :

Phosphate buffered saline with 0.1% bovine serum albumin (BSA)

conjugate :

Unconjugated

species :

Porcine

theoretical molecular weight :

19.1 kDa

details of functionality :

Interferon was titrated with the use of the cytopathic effect inhibition assay as listed . Bovine (MDBK/VSV) û performed as described [Rubinstein, et al. (1981) J. Virol. 37 (2):755]. The EC50 for interferon in this assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay. . The units are determined by use of a Human IFN Alpha A (Hu IFN-alpha A [2a]) laboratory standard calibrated to the international reference standard for Human Interferon Alpha A (Hu IFN-alpha A [2a]) provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257(1-2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NM_214393.1

applications :

Bioactivity

source long :

cDNA obtained from porcine leukocyte mRNA expressed in mammalian cells. Accession # NM_214393.1

USD :

556 USD

alt names :

IFNA, IFNA2, IFNA2b, IFNalpha, IFN-alphaA, INFA2, interferon alpha 2, LeIF D

storage :

Store the unopened product at -20 to -70 ░C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.