Recombinant Human Active Chk1 Protein, CF | R&D Systems 1630-KS-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Active Chk1 Protein, CF

catalog :

1630-KS-010

quantity :

10 ug

price :

534 USD

more info or order :

R&D Systems product webpage

citations: 3

Reference
Peng B, Shi R, Bian J, Li Y, Wang P, Wang H, et al. PARP1 and CHK1 coordinate PLK1 enzymatic activity during the DNA damage response to promote homologous recombination-mediated repair. Nucleic Acids Res. 2021;49:7554-7570 pubmed publisher
Yuan R, Vos H, van Es R, Chen J, Burgering B, Westendorp B, et al. Chk1 and 14-3-3 proteins inhibit atypical E2Fs to prevent a permanent cell cycle arrest. EMBO J. 2018;37: pubmed publisher
Li Z, Li X, Nai S, Geng Q, Liao J, Xu X, et al. Checkpoint kinase 1-induced phosphorylation of O-linked ?-N-acetylglucosamine transferase regulates the intermediate filament network during cytokinesis. J Biol Chem. 2017;292:19548-19555 pubmed publisher

product information

master code :

1630-KS

SKU :

1630-KS-010

product name :

Recombinant Human Active Chk1 Protein, CF

unit size :

10 ug

description :

The Recombinant Human Active Chk1 Protein, CF from R&D Systems is derived from Sf 9 (baculovirus). The Recombinant Human Active Chk1 Protein, CF has been validated for the following applications: Bioactivity.

target :

Chk1

category :

Proteins and Enzymes

buffer :

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain at 5 ╡g per lane.

species :

Human

observed molecular weight :

82 kDa

gene symbol :

CHEK1

details of functionality :

The specific activity of Chk1 was determined to be 228 nmol/min/mg using a synthetic peptide substrate (KKKVSRSGLYRSPSMPENLNRPR).

top caption :

Recombinant Human Active Chk1 Protein SDS-PAGE

accessionNumbers :

NM_001274

applications :

Bioactivity

source long :

Spodoptera frugiperda , Sf 9 (baculovirus)-derived human Chk1 protein

source short :

Sf 9 (baculovirus)

USD :

534 USD

alt names :

Checkpoint, S. pombe, homolog of, 1, CHEK1, CHK1 (checkpoint, S.pombe) homolog, CHK1 checkpoint homolog (S. pombe), CHK1serine/threonine-protein kinase Chk1, EC 2.7.11, EC 2.7.11.1

storage :

This product is stable at Avoid repeated freeze-thaw cycles.

more info or order :

R&D Systems product webpage