Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF | R&D Systems 161-DC product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

catalog :

161-DC

quantity :

50 ug

price :

369 USD

citations: 14

Reference
Mouser E, Pollakis G, Smits H, Thomas J, Yazdanbakhsh M, de Jong E, et al. Schistosoma mansoni soluble egg antigen (SEA) and recombinant Omega-1 modulate induced CD4+ T-lymphocyte responses and HIV-1 infection in vitro. PLoS Pathog. 2019;15:e1007924 pubmed publisher
Zhang X, Owens J, Olsen H, So E, Burch E, McCroskey M, et al. A recombinant human IgG1 Fc multimer designed to mimic the active fraction of IVIG in autoimmunity. JCI Insight. 2019;4: pubmed publisher
Eggink L, Roby K, Cote R, Kenneth Hoober J. An innovative immunotherapeutic strategy for ovarian cancer: CLEC10A and glycomimetic peptides. J Immunother Cancer. 2018;6:28 pubmed publisher
Zou Z, MISASI J, Sullivan N, Sun P. Overexpression of Ebola virus envelope GP1 protein. Protein Expr Purif. 2017;135:45-53 pubmed publisher
Mouser E, Pollakis G, Yazdanbakhsh M, Harnett W, de Jong E, Paxton W. Brugia malayi Antigen (BmA) Inhibits HIV-1 Trans-Infection but Neither BmA nor ES-62 Alter HIV-1 Infectivity of DC Induced CD4+ Th-Cells. PLoS ONE. 2016;11:e0146527 pubmed publisher
Peters M, Guidato P, Peters K, Megger D, Sitek B, Classen B, et al. Allergy-Protective Arabinogalactan Modulates Human Dendritic Cells via C-Type Lectins and Inhibition of NF-ÎºB. J Immunol. 2016;196:1626-35 pubmed publisher
Stax M, Mouser E, van Montfort T, Sanders R, de Vries H, Dekker H, et al. Colorectal mucus binds DC-SIGN and inhibits HIV-1 trans-infection of CD4+ T-lymphocytes. PLoS ONE. 2015;10:e0122020 pubmed publisher
Jiang Y, Zhang C, Chen K, Chen Z, Sun Z, Zhang Z, et al. The clinical significance of DC-SIGN and DC-SIGNR, which are novel markers expressed in human colon cancer. PLoS ONE. 2014;9:e114748 pubmed publisher
Emara M, Royer P, Mahdavi J, Shakib F, Ghaemmaghami A. Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite. J Biol Chem. 2012;287:5756-63 pubmed publisher
Johnson T, McLellan J, Graham B. Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2. J Virol. 2012;86:1339-47 pubmed publisher
Sabatte J, Faigle W, Ceballos A, Morelle W, Rodríguez Rodrígues C, Remes Lenicov F, et al. Semen clusterin is a novel DC-SIGN ligand. J Immunol. 2011;187:5299-309 pubmed publisher
Gustafsson A, Sjöblom M, Strindelius L, Johansson T, Fleckenstein T, Chatzissavidou N, et al. Pichia pastoris-produced mucin-type fusion proteins with multivalent O-glycan substitution as targeting molecules for mannose-specific receptors of the immune system. Glycobiology. 2011;21:1071-86 pubmed publisher
Stax M, van Montfort T, Sprenger R, Melchers M, Sanders R, van Leeuwen E, et al. Mucin 6 in seminal plasma binds DC-SIGN and potently blocks dendritic cell mediated transfer of HIV-1 to CD4(+) T-lymphocytes. Virology. 2009;391:203-11 pubmed publisher
Nonaka M, Ma B, Murai R, Nakamura N, Baba M, Kawasaki N, et al. Glycosylation-dependent interactions of C-type lectin DC-SIGN with colorectal tumor-associated Lewis glycans impair the function and differentiation of monocyte-derived dendritic cells. J Immunol. 2008;180:3347-56 pubmed

product information

brand :

R&D Systems

master code :

161-DC

SKU :

161-DC-050

product name :

Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

description :

The Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF from R&D Systems is derived from NS0. The Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF has been validated for the following applications: Bioactivity.

target :

DC-SIGN/CD209

category :

Proteins and Enzymes

unit size :

50 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

observed molecular weight :

65-75 kDa, reducing conditions

theoretical molecular weight :

65.7 kDa (monomer)

gene symbol :

CD209

details of functionality :

Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. When 5 x 104 cells/well are added to Recombinant Human DC-SIGN/CD209 Fc Chimera coated plates (5 µg/mL with 100 µL/well), approximately 50-80% of added cells will adhere after 1 hour at room temperature. Optimal dilutions should be determined by each laboratory for each application.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

catalog number base :

161-DC

accessionNumbers :

Q9NNX6

applications :

Bioactivity

source :

Mouse myeloma cell line, NS0-derived human DC-SIGN/CD209 protein MD Human IgG1 (Pro100-Lys330) IEGR Human DC-SIGN (Lys62-Ala404) Accession # Q9NNX6 N-terminus C-terminus

extended description :

A New and Improved rh DC-SIGN is Now Available! The new protein has enhanced purity and a HA-tag!

2020 USD :

369

2021 USD :

369 USD

product details :

A New and Improved rh DC-SIGN is Now Available! The new protein has enhanced purity and a HA-tag!

alt names :

CD209, CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin, CD209 molecule, CDSIGNHIV gpl20-binding protein, CLEC4L, CLEC4LC-type lectin domain family 4 member L, DCSIGN, DC-SIGN1, DC-SIGN1C-type lectin domain family 4, member L, DC-SIGNMGC129965, Dendritic cell-specific ICAM-3-grabbing non-integrin 1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.