Recombinant Drosophila Decapentaplegic (Dpp) Protein, CF | R&D Systems 159-DP-020/CF product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Drosophila Decapentaplegic (Dpp) Protein, CF

catalog :

159-DP-020/CF

quantity :

20 ug

price :

630 USD

more info or order :

R&D Systems product webpage

citations: 9

Reference
Liu S, Baeg G, Yang Y, Goh F, Bao H, Wagner E, et al. The Integrator complex desensitizes cellular response to TGF-β/BMP signaling. Cell Rep. 2023;42:112007 pubmed publisher
Neal S, Dolezal D, Jusić N, Pignoni F. Drosophila ML-DmD17-c3 cells respond robustly to Dpp and exhibit complex transcriptional feedback on BMP signaling components. BMC Dev Biol. 2019;19:1 pubmed publisher
Ammeux N, Housden B, Georgiadis A, Hu Y, Perrimon N. Mapping signaling pathway cross-talk in Drosophila cells. Proc Natl Acad Sci U S A. 2016;113:9940-5 pubmed publisher
Winstanley J, Sawala A, Baldock C, Ashe H. Synthetic enzyme-substrate tethering obviates the Tolloid-ECM interaction during Drosophila BMP gradient formation. elife. 2015;4: pubmed publisher
Zeng Z, de Gorter D, Kowalski M, Ten Dijke P, Shimmi O. Ter94/VCP is a novel component involved in BMP signaling. PLoS ONE. 2014;9:e114475 pubmed publisher
Donoughe S, Nakamura T, Ewen Campen B, Green D, Henderson L, Extavour C. BMP signaling is required for the generation of primordial germ cells in an insect. Proc Natl Acad Sci U S A. 2014;111:4133-8 pubmed publisher
Chen J, Honeyager S, Schleede J, Avanesov A, Laughon A, Blair S. Crossveinless d is a vitellogenin-like lipoprotein that binds BMPs and HSPGs, and is required for normal BMP signaling in the Drosophila wing. Development. 2012;139:2170-6 pubmed publisher
Wang Z, Flax L, Kemp M, Linhardt R, Baron M. Host and pathogen glycosaminoglycan-binding proteins modulate antimicrobial peptide responses in Drosophila melanogaster. Infect Immun. 2011;79:606-16 pubmed publisher
Serpe M, Umulis D, Ralston A, Chen J, Olson D, Avanesov A, et al. The BMP-binding protein Crossveinless 2 is a short-range, concentration-dependent, biphasic modulator of BMP signaling in Drosophila. Dev Cell. 2008;14:940-53 pubmed publisher

product information

brand :

R&D Systems

master code :

159-DP/CF

SKU :

159-DP-020/CF

product name :

Recombinant Drosophila Decapentaplegic (Dpp) Protein, CF

unit size :

20 ug

seo description :

The Recombinant Drosophila Decapentaplegic (Dpp) Protein, CF from R&D Systems is derived from E. coli. The Recombinant Drosophila Decapentaplegic (Dpp) Protein, CF has been validated for the following applications: Bioactivity.

target :

Decapentaplegic/DPP

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in Acetonitrile and TFA.

conjugate :

Unconjugated

dilution :

Bioactivity

purity :

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Drosophila

theoretical molecular weight :

14.8 kDa (monomer)

details of functionality :

Measured by its ability to induce Mad phosphorylation in S2 Drosophila cells transfected with Mad. Approximately 3 ╡g/mL of recombinant Drosophila DPP and higher can effectively induce Mad phosphorylation. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al . (1999) Exp. Cell Res. 250 :351. The ED50 for this effect is 0.5-2á╡g/mL.

endotoxin note :

<0.01 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_722813

applications :

Bioactivity

source :

E. coli-derived Recombinant Drosophila Decapentaplegic (Dpp) Protein, CF

USD :

630 USD

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage