domestic rabbit monoclonal (1042E)
conjugate: AF647
application: flow cytometry
citations: 2
conjugate: AF647
application: flow cytometry
citations: 2
Detection of DYKDDDDK (FLAG«) in HEK293 Human Cell Line Transfected with DYKDDDDK-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with DYKDDDDK-tagged proteins was stained with Rabbit Anti-DYKDDDDK Alexa Fluor« 647-conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC8529R, filled histogram) or rabbit IgG Alexa Fluor« 647 isotype control antibody (open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004 ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005 ). View our protocol for Staining Intracellular Molecules .
quantity: 25 Tests (also 100 Tests)
price: 149 USD
to the supplier
domestic rabbit monoclonal (1042E)
conjugate: biotin
application: western blot
conjugate: biotin
application: western blot
Detection of DYKDDDDK (FLAG«) Epitope Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20. PVDF membrane was probed with 0.1 ╡g/mL of Rabbit Anti-DYKDDDDK Biotinylated Monoclonal Antibody (Catalog # BAM8529) followed by Streptavidin-HRP (Catalog # DY998 ). Specific bands were detected for DYKDDDDK-tagged proteins at approximately 55 and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
quantity: 50 ug
price: 389 USD
to the supplier
domestic rabbit monoclonal (1042E)
conjugate: AF488
application: flow cytometry
conjugate: AF488
application: flow cytometry
Detection of DYKDDDDK (FLAG«) in HEK293 Human Cell Line Transfected with DYKDDDDK-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with DYKDDDDK-tagged proteins was stained with Rabbit Anti-DYKDDDDK Alexa Fluor« 488-conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC8529G, filled histogram) or isotype control antibody (Catalog # IC105G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004 ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005 ). View our protocol for Staining Intracellular Molecules .
quantity: 25 Tests (also 100 Tests)
price: 139 USD
to the supplier
domestic rabbit monoclonal (1042E)
conjugate: HRP
application: western blot
conjugate: HRP
application: western blot
Detection of DYKDDDDK (FLAG«) Epitope Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal DYKDDDDK-tagged Abro1 and N-terminal DYKDDDDK-tagged RNF20. PVDF membrane was probed with 1:1000-1:3000 dilution of Rabbit Anti-DYKDDDDK Epitope Tag HRP-conjugated Monoclonal Antibody (Catalog # HAM85291). Specific bands were detected for DYKDDDDK-tagged proteins at approximately 55 kDa and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
quantity: 0.5 mL
price: 289 USD
to the supplier