This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Insulin R/CD220 (aa 28-944) Protein
catalog :
1544-IR
quantity :
50 ug
price :
349 USD
citations: 4
Reference
Scapin G, Dandey V, Zhang Z, Prosise W, Hruza A, Kelly T, et al. Structure of the insulin receptor-insulin complex by single-particle cryo-EM analysis. Nature. 2018;556:122-125 pubmed publisher
Mukherjee S, Chattopadhyay M, Bhattacharya S, Dasgupta S, Hussain S, Bharadwaj S, et al. A Small Insulinomimetic Molecule Also Improves Insulin Sensitivity in Diabetic Mice. PLoS ONE. 2017;12:e0169809 pubmed publisher
Corbin J, Bhaskar V, Goldfine I, Issafras H, Bedinger D, Lau A, et al. Inhibition of insulin receptor function by a human, allosteric monoclonal antibody: a potential new approach for the treatment of hyperinsulinemic hypoglycemia. MAbs. 2014;6:262-72 pubmed publisher
Umehara A, Nishioka M, Obata T, Ebina Y, Shiota H, Hashida S. A novel ultra-sensitive enzyme immunoassay for soluble human insulin receptor ectodomain and its measurement in urine from healthy subjects and patients with diabetes mellitus. Clin Biochem. 2009;42:1468-75 pubmed publisher
product information
brand :
R&D Systems
master code :
1544-IR
SKU :
1544-IR-050
product name :
Recombinant Human Insulin R/CD220 (aa 28-944) Protein
description :
The Recombinant Human Insulin R/CD220 (aa 28-944) Protein from R&D Systems is derived from NS0. The Recombinant Human Insulin R/CD220 (aa 28-944) Protein has been validated for the following applications: Binding Activity.
target :
Insulin R/CD220
category :
Proteins and Enzymes
unit size :
50 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
observed molecular weight :
122-135 kDa and 33-43 kDa, reducing conditions
theoretical molecular weight :
82.9 kDa ( alpha subunit), 22.9 kDa ( beta subunit)
gene symbol :
INSR
details of functionality :
Measured by its binding ability in a functional ELISA. When 15 ng/mL of biotinylated recombinant human Insulin is added to serially diluted Recombinant Human Insulin R/CD220, the concentration of Recombinant Human Insulin R/CD220 that produces 50% of the optimal binding response is 0.03-0.15 µg/mL.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
catalog number base :
1544-IR
accessionNumbers :
P06213-2
applications :
Binding Activity
source :
Mouse myeloma cell line, NS0-derived human Insulin R/CD220 protein His28-Arg750 ( alpha subunit) & Ser751-Lys944 with a C-terminal 10-His tag ( beta subunit)
2020 USD :
349
2021 USD :
349 USD
alt names :
CD 220, CD220, CD220 antigen, EC 2.7.10, EC 2.7.10.1, HHF5, INSR, insulin receptor, InsulinR, IR
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.