Recombinant Human Insulin R/CD220 (aa 28-944) Protein, CF | R&D Systems 1544-IR-050/CF product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Insulin R/CD220 (aa 28-944) Protein, CF

catalog :

1544-IR-050/CF

quantity :

50 ug

price :

405 USD

more info or order :

R&D Systems product webpage

citations: 7

Reference
Yunn N, Lee J, Lee H, Oh E, Park M, Park S, et al. An aptamer agonist of the insulin receptor acts as a positive or negative allosteric modulator, depending on its concentration. Exp Mol Med. 2022;54:531-541 pubmed publisher
Yunn N, Park M, Park S, Lee J, Noh J, Shin E, et al. A hotspot for enhancing insulin receptor activation revealed by a conformation-specific allosteric aptamer. Nucleic Acids Res. 2021;49:700-712 pubmed publisher
Lee L, Khakoo A, Chintalgattu V. Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease. FEBS Open Bio. 2021;11:207-225 pubmed publisher
Scapin G, Dandey V, Zhang Z, Prosise W, Hruza A, Kelly T, et al. Structure of the insulin receptor-insulin complex by single-particle cryo-EM analysis. Nature. 2018;556:122-125 pubmed publisher
Mukherjee S, Chattopadhyay M, Bhattacharya S, Dasgupta S, Hussain S, Bharadwaj S, et al. A Small Insulinomimetic Molecule Also Improves Insulin Sensitivity in Diabetic Mice. PLoS ONE. 2017;12:e0169809 pubmed publisher
Corbin J, Bhaskar V, Goldfine I, Issafras H, Bedinger D, Lau A, et al. Inhibition of insulin receptor function by a human, allosteric monoclonal antibody: a potential new approach for the treatment of hyperinsulinemic hypoglycemia. MAbs. 2014;6:262-72 pubmed publisher
Umehara A, Nishioka M, Obata T, Ebina Y, Shiota H, Hashida S. A novel ultra-sensitive enzyme immunoassay for soluble human insulin receptor ectodomain and its measurement in urine from healthy subjects and patients with diabetes mellitus. Clin Biochem. 2009;42:1468-75 pubmed publisher

product information

brand :

R&D Systems

master code :

1544-IR/CF

SKU :

1544-IR-050/CF

product name :

Recombinant Human Insulin R/CD220 (aa 28-944) Protein, CF

description :

The Recombinant Human Insulin R/CD220 (aa 28-944) Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Insulin R/CD220 (aa 28-944) Protein, CF has been validated for the following applications: Binding Activity.

target :

Insulin R/CD220

category :

Proteins and Enzymes

unit sizes :

50 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

observed molecular weight :

122-135 kDa and 33-43 kDa, reducing conditions

theoretical molecular weight :

82.9 kDa ( alpha subunit), 22.9 kDa ( beta subunit)

gene symbol :

INSR

details of functionality :

Measured by its binding ability in a functional ELISA. When 15 ng/mL of biotinylated recombinant human Insulin is added to serially diluted Recombinant Human Insulin R/CD220, the concentration of Recombinant Human Insulin R/CD220 that produces 50% of the optimal binding response is 0.03-0.15 µg/mL.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P06213-2

applications :

Binding Activity

source :

NS0-derived Recombinant Human Insulin R/CD220 (aa 28-944) Protein, CF

USD :

405 USD

alt names :

CD 220, CD220, CD220 antigen, EC 2.7.10, EC 2.7.10.1, HHF5, INSR, insulin receptor, InsulinR, IR

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage