This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
other
product name :
Recombinant Human Osteopontin (OPN) Protein, CF
catalog :
1433-OP-050/CF
quantity :
50 ug
price :
249 USD
product information
brand :
R&D Systems
master code :
1433-OP/CF
SKU :
1433-OP-050/CF
product name :
Recombinant Human Osteopontin (OPN) Protein, CF
description :
The Recombinant Human Osteopontin (OPN) Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Osteopontin (OPN) Protein, CF has been validated for the following applications: Bioactivity.
target :
Osteopontin/OPN
category :
Proteins and Enzymes
unit size :
50 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
observed molecular weight :
60-65 kDa, reducing conditions
theoretical molecular weight :
32.9 kDa
gene symbol :
SPP1
details of functionality :
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Agnihotri, R. et al . (2001) J. Biol. Chem. 276 :28261. When 1 x 105 cells/well are added to a Recombinant Human (rh) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is 0.1-0.6 µg/mL. Recombinant Human Coagulation Factor II/Thrombin (Catalog # 1473-SE ) proteolytic treatment of this rhOsteopontin/His can increase HEK293 cell adhesion by about 5-fold.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
NP_000573.1
applications :
Bioactivity
source :
The Recombinant Human Osteopontin (OPN) Protein, CF from R&D Systems is derived from Mouse myeloma cell line, NS0-derived human Osteopontin/OPN protein Ile17-Asn300, with a C-terminal 6-His tag. The Recombinant Human Osteopontin (OPN) Protein, CF has been validated for the following applications: Bioactivity.
USD 2021 :
239
USD 2022 :
249 USD
alt name :
BNSP, Bone sialoprotein 1, Eta-1, MGC110940, Nephropontin, OPN, osteopontin, secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1), secreted phosphoprotein-1 (osteopontin, bone sialoprotein), Spp1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, uropontin
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.