Recombinant Human Cystatin B Protein, CF | R&D Systems 1408-PI-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Cystatin B Protein, CF

catalog :

1408-PI-050

quantity :

50 ug

price :

432 USD

more info or order :

R&D Systems product webpage

citations: 4

Reference
Hu J, Gou T, Wu W, Sun J, Zhang S, Zhou S, et al. Proximity ligation assays for precise quantification of femtomolar proteins in single cells using self-priming microfluidic dPCR chip. Anal Chim Acta. 2019;1076:118-124 pubmed publisher
Kasabova M, Joulin Giet A, Lecaille F, Gilmore B, Marchand Adam S, Saidi A, et al. Regulation of TGF-?1-driven differentiation of human lung fibroblasts: emerging roles of cathepsin B and cystatin C. J Biol Chem. 2014;289:16239-51 pubmed publisher
Cheng T, Hitomi K, van Vlijmen Willems I, de Jongh G, Yamamoto K, Nishi K, et al. Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification. J Biol Chem. 2006;281:15893-9 pubmed
Johnson G, Jiang W. Characterization of cathepsin L secreted by Sf21 insect cells. Arch Biochem Biophys. 2005;444:7-14 pubmed

product information

master code :

1408-PI

SKU :

1408-PI-050

product name :

Recombinant Human Cystatin B Protein, CF

unit size :

50 ug

description :

The Recombinant Human Cystatin B Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human Cystatin B Protein, CF has been validated for the following applications: Inhibition Activity.

target :

Cystatin B

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in Tris and NaCl.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

observed molecular weight :

13 kDa, reducing conditions

theoretical molecular weight :

12 kDa

gene symbol :

CSTB

details of functionality :

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (Catalog # ES009 ). The IC50 value is 15 nM, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P04080

applications :

Inhibition Activity

source long :

E. coli -derived human Cystatin B protein Met2-Phe98, with an N-terminal Met and 7-His tag

source short :

E. coli

USD :

432 USD

alt names :

CPI-B, CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B, CSTB, cystatin B (stefin B), EPM1, Liver thiol proteinase inhibitor, PME, stefin B, stefin-B

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage