Recombinant Human Proinsulin Protein, CF | R&D Systems 1336-PN-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Proinsulin Protein, CF

catalog :

1336-PN-050

quantity :

50 ug

price :

394 USD

more info or order :

R&D Systems product webpage

citations: 10

Reference
Meier D, Rachid L, Wiedemann S, Traub S, Trimigliozzi K, Stawiski M, et al. Prohormone convertase 1/3 deficiency causes obesity due to impaired proinsulin processing. Nat Commun. 2022;13:4761 pubmed publisher
Viviano J, Brecker M, Ferrara Cook C, Suaud L, Rubenstein R. ERp29 as a regulator of Insulin biosynthesis. PLoS ONE. 2020;15:e0233502 pubmed publisher
Toledo P, Torkko J, Muller A, Wegbrod C, Sönmez A, Solimena M, et al. ICA512 RESP18 homology domain is a protein-condensing factor and insulin fibrillation inhibitor. J Biol Chem. 2019;294:8564-8576 pubmed publisher
Liu Y, Wang H, Zhou L, Su Y, Shen W. Biodistribution, activation, and retention of proinsulin-transferrin fusion protein in the liver: Mechanism of liver-targeting as an insulin prodrug. J Control Release. 2018;275:186-191 pubmed publisher
Hsieh Y, Chien K, Yang I, Lee I, Wu C, Huang T, et al. Oxidation of protein-bound methionine in Photofrin-photodynamic therapy-treated human tumor cells explored by methionine-containing peptide enrichment and quantitative proteomics approach. Sci Rep. 2017;7:1370 pubmed publisher
Farino Z, Morgenstern T, Vallaghe J, Gregor N, Donthamsetti P, Harris P, et al. Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence. PLoS ONE. 2016;11:e0148684 pubmed publisher
Rulifson I, Cao P, Miao L, Kopecky D, Huang L, White R, et al. Identification of Human Islet Amyloid Polypeptide as a BACE2 Substrate. PLoS ONE. 2016;11:e0147254 pubmed publisher
Mbongue J, Nicholas D, Zhang K, Kim N, Hamilton B, Larios M, et al. Induction of indoleamine 2, 3-dioxygenase in human dendritic cells by a cholera toxin B subunit-proinsulin vaccine. PLoS ONE. 2015;10:e0118562 pubmed publisher
Nardi F, Lipina C, Magill D, Hage Hassan R, Hajduch E, Gray A, et al. Enhanced insulin sensitivity associated with provision of mono and polyunsaturated fatty acids in skeletal muscle cells involves counter modulation of PP2A. PLoS ONE. 2014;9:e92255 pubmed publisher
Wang Y, Shao J, Zaro J, Shen W. Proinsulin-transferrin fusion protein as a novel long-acting insulin analog for the inhibition of hepatic glucose production. Diabetes. 2014;63:1779-88 pubmed publisher

product information

brand :

R&D Systems

master code :

1336-PN

SKU :

1336-PN-050

product name :

Recombinant Human Proinsulin Protein, CF

description :

The Recombinant Human Proinsulin Protein, CF from R&D Systems is derived from E. coli. The Recombinant Human Proinsulin Protein, CF has been validated for the following applications: Bioactivity.

target :

Proinsulin

category :

Proteins and Enzymes

sizes available :

50 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

observed molecular weight :

9 kDa, reducing conditions

theoretical molecular weight :

10.5 kDa

gene symbol :

INS

details of functionality :

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al . (1988) Cancer Research 48 :4083. The ED50 for this effect is 0.15-0.75 µg/mL.

endotoxin note :

<0.01 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

NP_000198

applications :

Bioactivity

source :

E. coli-derived Recombinant Human Proinsulin Protein, CF

USD :

388

USD 2023 :

394 USD

alt names :

IDDM2, ILPR, insulin, IRDN, MODY10, proinsulin

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage