Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein | R&D Systems 1314-SH-025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

catalog :

1314-SH-025

quantity :

25 ug

price :

286 USD

citations: 16

Reference
Maimaitili M, Chen M, Febbraro F, Ucuncu E, Kelly R, Niclis J, et al. Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells. Nat Commun. 2023;14:7871 pubmed publisher
Lee J, Kim Y, Ataliotis P, Kim H, Kim D, Bennett D, et al. Coordination of canonical and noncanonical Hedgehog signalling pathways mediated by WDR11 during primordial germ cell development. Sci Rep. 2023;13:12309 pubmed publisher
Sun J, Shin D, Eiseman M, Yallowitz A, Li N, Lalani S, et al. SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts. Nat Commun. 2021;12:4611 pubmed publisher
Kim Y, Lee J, Seppala M, Cobourne M, Kim S. Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration. Nat Commun. 2020;11:1994 pubmed publisher
Arribat Y, Mysiak K, Lescouzères L, Boizot A, Ruiz M, Rossel M, et al. Sonic Hedgehog repression underlies gigaxonin mutation-induced motor deficits in giant axonal neuropathy. J Clin Invest. 2019;129:5312-5326 pubmed publisher
Johansen T, Krabbe C, Schmidt S, Serrano A, Meyer M. Comparative Analysis of Spontaneous and Stimulus-Evoked Calcium Transients in Proliferating and Differentiating Human Midbrain-Derived Stem Cells. Stem Cells Int. 2017;2017:9605432 pubmed publisher
El Agha E, Kheirollahi V, Moiseenko A, Seeger W, Bellusci S. Ex vivo analysis of the contribution of FGF10+ cells to airway smooth muscle cell formation during early lung development. Dev Dyn. 2017;246:531-538 pubmed publisher
Dye B, Hill D, Ferguson M, Tsai Y, Nagy M, Dyal R, et al. In vitro generation of human pluripotent stem cell derived lung organoids. elife. 2015;4: pubmed publisher
Xu Y, An Y, Wang X, Zha W, Li X. Inhibition of the Hedgehog pathway induces autophagy in pancreatic ductal adenocarcinoma cells. Oncol Rep. 2014;31:707-12 pubmed publisher
Mills L, Zhang Y, Marler R, Herreros Villanueva M, Zhang L, Almada L, et al. Loss of the transcription factor GLI1 identifies a signaling network in the tumor microenvironment mediating KRAS oncogene-induced transformation. J Biol Chem. 2013;288:11786-94 pubmed publisher
Aad P, Echternkamp S, Sypherd D, Schreiber N, Spicer L. The hedgehog system in ovarian follicles of cattle selected for twin ovulations and births: evidence of a link between the IGF and hedgehog systems. Biol Reprod. 2012;87:79 pubmed publisher
Erceg S, Lukovic D, Moreno Manzano V, Stojkovic M, Bhattacharya S. Derivation of cerebellar neurons from human pluripotent stem cells. Curr Protoc Stem Cell Biol. 2012;Chapter 1:Unit 1H.5 pubmed publisher
Gil J, Woo D, Shim J, Kim S, You H, Park S, et al. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells. FEBS Lett. 2009;583:561-7 pubmed publisher
Elkabetz Y, Panagiotakos G, Al Shamy G, Socci N, Tabar V, Studer L. Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage. Genes Dev. 2008;22:152-65 pubmed publisher
Zavros Y, Waghray M, Tessier A, Bai L, Todisco A, L Gumucio D, et al. Reduced pepsin A processing of sonic hedgehog in parietal cells precedes gastric atrophy and transformation. J Biol Chem. 2007;282:33265-74 pubmed
Nordström U, Maier E, Jessell T, Edlund T. An early role for WNT signaling in specifying neural patterns of Cdx and Hox gene expression and motor neuron subtype identity. PLoS Biol. 2006;4:e252 pubmed

product information

master code :

1314-SH

SKU :

1314-SH-025

product name :

Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein

unit size :

25 ug

description :

The Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein from R&D Systems is derived from E. coli. The Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein has been validated for the following applications: Bioactivity.

target :

Sonic Hedgehog/Shh

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in NaH2PO4, NaCl and DTT with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE with silver staining, under reducing conditions

species :

Human

observed molecular weight :

22 kDa, reducing conditions

theoretical molecular weight :

20 kDa

gene symbol :

SHH

details of functionality :

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al . (1997) Biochem. Biophys. Res. Commun. 237 :465. The ED50 for this effect is 5 ╡g/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

top caption :

Recombinant Human Sonic Hedgehog/Shh, N-Terminus Protein Bioactivity

accessionNumbers :

Q15465

applications :

Bioactivity

source long :

E. coli -derived human Sonic Hedgehog/Shh protein Cys24-Gly197, with a C-terminal 6-His tag

source short :

E. coli

USD :

286 USD

alt names :

HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.