product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Sonic Hedgehog/Shh, N-Terminus GMP, CF
catalog :
1314-GMP-025
quantity :
25 ug (also 1 mg)
price :
573 USD
more info or order :
R&D Systems product webpage
product information
master code :
1314-GMP
SKU :
1314-GMP-025
product name :
Recombinant Human Sonic Hedgehog/Shh, N-Terminus GMP, CF
unit size :
25 ug (also 1 mg)
description :
The Recombinant Human Sonic Hedgehog/Shh, N-Terminus GMP, CF from R&D Systems is derived from E. coli. The Recombinant Human Sonic Hedgehog/Shh, N-Terminus GMP, CF has been validated for the following applications: Bioactivity.
target :
Sonic Hedgehog/Shh
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in NaH2PO4, NaCl and DTT .
conjugate :
Unconjugated
purity :
97%, by SDS-PAGE with silver staining, under reducing conditions. Intact mass analysis of recombinant human sonic hedgehog confirms the predicted molecular mass of 20425 Da
species :
Human
observed molecular weight :
22 kDa, reducing conditions
theoretical molecular weight :
20 kDa
gene symbol :
SHH
details of functionality :
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al . (1997) Biochem. Biophys. Res. Commun. 237 :465. The ED50 for this effect is 5 ╡g/mL.
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant Human Sonic Hedgehog/Shh, N-Terminus GMP Bioactivity
accessionNumbers :
Q15465.1
applications :
Bioactivity
source long :
E. coli -derived human Sonic Hedgehog/Shh protein Cys24-Gly197, with a C-terminal 6-His tag Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.
source short :
E. coli
extended description :
Animal-Free
USD :
573 USD
product details :
Animal-Free
alt names :
HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at <= -20 ░C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, <= -20 ░C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.