Recombinant Human SLPI Protein, CF | R&D Systems 1274-PI-100 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human SLPI Protein, CF

catalog :

1274-PI-100

quantity :

100 ug

price :

291 USD

more info or order :

R&D Systems product webpage

citations: 23

Reference
Lebeau A, Bruyere D, Roncarati P, Peixoto P, Hervouet E, Cobraiville G, et al. HPV infection alters vaginal microbiome through down-regulating host mucosal innate peptides used by Lactobacilli as amino acid sources. Nat Commun. 2022;13:1076 pubmed publisher
Biswas S, Chen E, Gao Y, Lee S, Hewlett I, Devadas K. Modulation of HIV Replication in Monocyte-Derived Macrophages (MDM) by Host Antiviral Factors Secretory Leukocyte Protease Inhibitor and Serpin Family C Member 1 Induced by Steroid Hormones. Viruses. 2022;14: pubmed publisher
Kim K, Turner R, Khazan N, Kodza A, Jones A, Singh R, et al. Role of trypsin and protease-activated receptor-2 in ovarian cancer. PLoS ONE. 2020;15:e0232253 pubmed publisher
Molloy K, Smith S, Cagney G, Dillon E, Greene C, McElvaney N. Characterisation of the Major Extracellular Proteases of Stenotrophomonas maltophilia and Their Effects on Pulmonary Antiproteases. Pathogens. 2019;8: pubmed publisher
Silvestre Roig C, Braster Q, Wichapong K, LEE E, Teulon J, Berrebeh N, et al. Externalized histone H4 orchestrates chronic inflammation by inducing lytic cell death. Nature. 2019;569:236-240 pubmed publisher
Triantafyllou E, Pop O, Possamai L, Wilhelm A, Liaskou E, Singanayagam A, et al. MerTK expressing hepatic macrophages promote the resolution of inflammation in acute liver failure. Gut. 2018;67:333-347 pubmed publisher
Vago J, Tavares L, Sugimoto M, Lima G, GalvÃ£o I, de Caux T, et al. Proresolving Actions of Synthetic and Natural Protease Inhibitors Are Mediated by Annexin A1. J Immunol. 2016;196:1922-32 pubmed publisher
Stapels D, Ramyar K, Bischoff M, von KÃ¶ckritz Blickwede M, Milder F, Ruyken M, et al. Staphylococcus aureus secretes a unique class of neutrophil serine protease inhibitors. Proc Natl Acad Sci U S A. 2014;111:13187-92 pubmed publisher
Yang Y, Rhodus N, Ondrey F, Wuertz B, Chen X, Zhu Y, et al. Quantitative proteomic analysis of oral brush biopsies identifies secretory leukocyte protease inhibitor as a promising, mechanism-based oral cancer biomarker. PLoS ONE. 2014;9:e95389 pubmed publisher
Woodham A, Raff A, Raff L, Da Silva D, Yan L, Skeate J, et al. Inhibition of Langerhans cell maturation by human papillomavirus type 16: a novel role for the annexin A2 heterotetramer in immune suppression. J Immunol. 2014;192:4748-57 pubmed publisher
Labidi Galy S, Clauss A, Ng V, Duraisamy S, Elias K, Piao H, et al. Elafin drives poor outcome in high-grade serous ovarian cancers and basal-like breast tumors. Oncogene. 2015;34:373-83 pubmed publisher
Hannila S, Siddiq M, Carmel J, Hou J, Chaudhry N, Bradley P, et al. Secretory leukocyte protease inhibitor reverses inhibition by CNS myelin, promotes regeneration in the optic nerve, and suppresses expression of the transforming growth factor-β signaling protein Smad2. J Neurosci. 2013;33:5138-51 pubmed publisher
Suh H, Choi N, Tarassishin L, Lee S. Regulation of progranulin expression in human microglia and proteolysis of progranulin by matrix metalloproteinase-12 (MMP-12). PLoS ONE. 2012;7:e35115 pubmed publisher
Müller A, Jun E, Conlon H, Sadiq S. Inhibition of SLPI ameliorates disease activity in experimental autoimmune encephalomyelitis. BMC Neurosci. 2012;13:30 pubmed publisher
Vega Carrascal I, Reeves E, Niki T, Arikawa T, McNally P, O Neill S, et al. Dysregulation of TIM-3-galectin-9 pathway in the cystic fibrosis airways. J Immunol. 2011;186:2897-909 pubmed publisher
Nicol G, Han M, Kim J, Birse C, Brand E, Nguyen A, et al. Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients. Mol Cell Proteomics. 2008;7:1974-82 pubmed publisher
Jacobsen L, Sørensen O, Cowland J, Borregaard N, Theilgaard Monch K. The secretory leukocyte protease inhibitor (SLPI) and the secondary granule protein lactoferrin are synthesized in myelocytes, colocalize in subcellular fractions of neutrophils, and are coreleased by activated neutrophils. J Leukoc Biol. 2008;83:1155-64 pubmed publisher
Simpkins F, Devoogdt N, Rasool N, Tchabo N, Alejandro E, Kamrava M, et al. The alarm anti-protease, secretory leukocyte protease inhibitor, is a proliferation and survival factor for ovarian cancer cells. Carcinogenesis. 2008;29:466-72 pubmed
Fernie King B, Seilly D, Binks M, Sriprakash K, Lachmann P. Streptococcal DRS (distantly related to SIC) and SIC inhibit antimicrobial peptides, components of mucosal innate immunity: a comparison of their activities. Microbes Infect. 2007;9:300-7 pubmed
Xu W, He B, Chiu A, Chadburn A, Shan M, Buldys M, et al. Epithelial cells trigger frontline immunoglobulin class switching through a pathway regulated by the inhibitor SLPI. Nat Immunol. 2007;8:294-303 pubmed
Into T, Inomata M, Kanno Y, Matsuyama T, Machigashira M, Izumi Y, et al. Arginine-specific gingipains from Porphyromonas gingivalis deprive protective functions of secretory leucocyte protease inhibitor in periodontal tissue. Clin Exp Immunol. 2006;145:545-54 pubmed
Cortesio C, Jiang W. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Arch Biochem Biophys. 2006;449:164-70 pubmed
Tam E, Morrison C, Wu Y, Stack M, Overall C. Membrane protease proteomics: Isotope-coded affinity tag MS identification of undescribed MT1-matrix metalloproteinase substrates. Proc Natl Acad Sci U S A. 2004;101:6917-22 pubmed

product information

brand :

R&D Systems

master code :

1274-PI

SKU :

1274-PI-100

product name :

Recombinant Human SLPI Protein, CF

unit size :

100 ug

description :

The Recombinant Human IL-36Ra/IL-1F5 Protein from R&D Systems is derived from E. coli. The Recombinant Human IL-36Ra/IL-1F5 Protein has been validated for the following applications: Bioactivity.

target :

SLPI

category :

Proteins and Enzymes

alt names :

ALK1, ALPProtease inhibitor WAP4, antileukoproteinase, BLPISeminal proteinase inhibitor, HUSI, HUSI-I, Mucus proteinase inhibitor, secretory leukocyte peptidase inhibitor, Secretory leukocyte protease inhibitor, secretory leukocyte protease inhibitor (antileukoproteinase), WAP four-disulfide core domain protein 4, WAP4HUSI-1, WFDC4MPI

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS.

conjugate :

Unconjugated

all available conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

observed molecular weight :

13 kDa, reducing conditions

theoretical molecular weight :

12 kDa

gene symbol :

SLPI

details of functionality :

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002 ). The IC50 value is 4.0 nM, under the described conditions.

endotoxin note :

<1.0 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P03973

applications :

Inhibition Activity

source :

E. coli-derived Recombinant Human SLPI Protein, CF

USD :

269

Jul-22 :

291 USD

sizes available :

100 ug

more info or order :

R&D Systems product webpage