Recombinant Human Netrin-4 Protein | R&D Systems 1254-N4-025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Netrin-4 Protein

catalog :

1254-N4-025

quantity :

25 ug

price :

498 USD

citations: 10

Reference
Bai Z, Bartelo N, Aslam M, Hale C, Blachere N, Parveen S, et al. Machine Learning Reveals Synovial Fibroblast Genes Associated with Pain Affect Sensory Nerve Growth in Rheumatoid Arthritis. medRxiv. 2023;: pubmed publisher
Zhang H, Vreeken D, Leuning D, Bruikman C, Junaid A, Stam W, et al. Netrin-4 expression by human endothelial cells inhibits endothelial inflammation and senescence. Int J Biochem Cell Biol. 2021;134:105960 pubmed publisher
Naldaiz Gastesi N, Goicoechea M, Aragón I, Pérez López V, Fuertes Alvarez S, Herrera Imbroda B, et al. Isolation and characterization of myogenic precursor cells from human cremaster muscle. Sci Rep. 2019;9:3454 pubmed publisher
Li L, Huang Y, Gao Y, Shi T, Xu Y, Li H, et al. EGF/EGFR upregulates and cooperates with Netrin-4 to protect glioblastoma cells from DNA damage-induced senescence. BMC Cancer. 2018;18:1215 pubmed publisher
Villanueva A, Puvogel S, Lois P, Muñoz Palma E, Ramírez Orellana M, Lubieniecki F, et al. The Netrin-4/Laminin γ1/Neogenin-1 complex mediates migration in SK-N-SH neuroblastoma cells. Cell Adh Migr. 2019;13:33-40 pubmed publisher
Villanueva A, FalcÃ³n P, Espinoza N, R L, Milla L, Hernández SanMiguel E, et al. The Netrin-4/ Neogenin-1 axis promotes neuroblastoma cell survival and migration. Oncotarget. 2017;8:9767-9782 pubmed publisher
Akino T, Han X, Nakayama H, McNeish B, Zurakowski D, Mammoto A, et al. Netrin-1 promotes medulloblastoma cell invasiveness and angiogenesis, and demonstrates elevated expression in tumor tissue and urine of patients with pediatric medulloblastoma. Cancer Res. 2014;74:3716-26 pubmed publisher
Li L, Hu Y, Ylivinkka I, Li H, Chen P, Keski Oja J, et al. NETRIN-4 protects glioblastoma cells FROM temozolomide induced senescence. PLoS ONE. 2013;8:e80363 pubmed publisher
Sneddon J, Borowiak M, Melton D. Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme. Nature. 2012;491:765-8 pubmed publisher
Lejmi E, Leconte L, Pedron Mazoyer S, Ropert S, Raoul W, Lavalette S, et al. Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B. Proc Natl Acad Sci U S A. 2008;105:12491-6 pubmed publisher

product information

master code :

1254-N4

SKU :

1254-N4-025

product name :

Recombinant Human Netrin-4 Protein

unit size :

25 ug

description :

The Recombinant Human Netrin-4 Protein from R&D Systems is derived from NS0. The Recombinant Human Netrin-4 Protein has been validated for the following applications: Binding Activity.

target :

Netrin-4

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

observed molecular weight :

90 kDa, reducing conditions

theoretical molecular weight :

69 kDa

gene symbol :

NTN4

details of functionality :

Measured by its binding ability in a functional ELISA. Immobilized rrUNC5H2/Fc Chimera at 5 ╡g/mL (100 ╡L/well) can bind rhNetrin-4 with a linear range of 6-400 ng/mL.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q9HB63.2

applications :

Binding Activity

source long :

Mouse myeloma cell line, NS0-derived human Netrin-4 protein Val26-Lys628, with a C-terminal 10-His tag

source short :

NS0

USD :

498 USD

alt names :

beta-netrin, hepar-derived netrin-like protein, netrin 4, Netrin4, NTN4, PRO3091

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.