Recombinant Human Semaphorin 3A Fc Chimera Protein, CF | R&D Systems 1250-S3-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Semaphorin 3A Fc Chimera Protein, CF

catalog :

1250-S3-025

quantity :

25 ug

price :

436 USD

more info or order :

R&D Systems product webpage

citations: 34

Reference
Koralewska N, Corradi E, Milewski M, Masante L, Szczepanska A, Kierzek R, et al. Short 2'-O-methyl/LNA oligomers as highly-selective inhibitors of miRNA production in vitro and in vivo. Nucleic Acids Res. 2024;52:5804-5824 pubmed publisher
Jeon H, Shin Y, Lee J, Chang N, Woo D, Lee W, et al. The semaphorin 3A/neuropilin-1 pathway promotes clonogenic growth of glioblastoma via activation of TGF-β signaling. JCI Insight. 2023;8: pubmed publisher
Sen S, Lux C, Erber R. A Potential Role of Semaphorin 3A during Orthodontic Tooth Movement. Int J Mol Sci. 2021;22: pubmed publisher
Ivakhnitskaia E, Chin M, Siegel D, Guaiquil V. Vinaxanthone inhibits Semaphorin3A induced axonal growth cone collapse in embryonic neurons but fails to block its growth promoting effects on adult neurons. Sci Rep. 2021;11:13019 pubmed publisher
Limoni G, Murthy S, Jabaudon D, Dayer A, Niquille M. PlexinA4-Semaphorin3A-mediated crosstalk between main cortical interneuron classes is required for superficial interneuron lamination. Cell Rep. 2021;34:108644 pubmed publisher
Higgins D, Caliva M, Schroeder M, Carlson B, Upadhyayula P, Milligan B, et al. Semaphorin 3A mediated brain tumor stem cell proliferation and invasion in EGFRviii mutant gliomas. BMC Cancer. 2020;20:1213 pubmed publisher
Nadanaka S, Miyata S, Yaqiang B, Tamura J, Habuchi O, Kitagawa H. Reconsideration of the Semaphorin-3A Binding Motif Found in Chondroitin Sulfate Using Galnac4s-6st-Knockout Mice. Biomolecules. 2020;10: pubmed publisher
Leclerc M, Voilin E, Gros G, Corgnac S, de Montpreville V, Validire P, et al. Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1. Nat Commun. 2019;10:3345 pubmed publisher
Pavez M, Thompson A, Arnott H, Mitchell C, D Atri I, Don E, et al. STIM1 is required for remodelling of the endoplasmic reticulum and microtubule cytoskeleton in steering growth cones. J Neurosci. 2019;: pubmed publisher
Podojil J, Chiang M, Ifergan I, Copeland R, Liu L, Maloveste S, et al. B7-H4 Modulates Regulatory CD4+ T Cell Induction and Function via Ligation of a Semaphorin 3a/Plexin A4/Neuropilin-1 Complex. J Immunol. 2018;201:897-907 pubmed publisher
Grindel B, Martinez J, Tellman T, Harrington D, Zafar H, Nakhleh L, et al. Matrilysin/MMP-7 Cleavage of Perlecan/HSPG2 Complexed with Semaphorin 3A Supports FAK-Mediated Stromal Invasion by Prostate Cancer Cells. Sci Rep. 2018;8:7262 pubmed publisher
de Ridder D, Marino S, Bishop R, Renema N, Chenu C, Heymann D, et al. Bidirectional regulation of bone formation by exogenous and osteosarcoma-derived Sema3A. Sci Rep. 2018;8:6877 pubmed publisher
Tang M, Malvar Fernandez B, Newsom S, van Buul J, Radstake T, Baeten D, et al. Class 3 semaphorins modulate the invasive capacity of rheumatoid arthritis fibroblast-like synoviocytes. Rheumatology (Oxford). 2018;57:909-920 pubmed publisher
Zhang M, Zhou Q, Luo Y, Nguyen T, Rosenblatt M, Guaiquil V. Semaphorin3A induces nerve regeneration in the adult cornea-a switch from its repulsive role in development. PLoS ONE. 2018;13:e0191962 pubmed publisher
Yokoi T, Tanaka T, Matsuzaka E, Tamalu F, Watanabe S, Nishina S, et al. Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells. Sci Rep. 2017;7:16757 pubmed publisher
Hong B, You S, Yoo S, Park D, Hwang D, Cho C, et al. MicroRNA-143 and -145 modulate the phenotype of synovial fibroblasts in rheumatoid arthritis. Exp Mol Med. 2017;49:e363 pubmed publisher
Palodetto B, da Silva Santos Duarte A, Rodrigues Lopes M, Adolfo Corrocher F, Marconi Roversi F, Soares Niemann F, et al. SEMA3A partially reverses VEGF effects through binding to neuropilin-1. Stem Cell Res. 2017;22:70-78 pubmed publisher
Gehler S, Compere F, Miller A. Semaphorin 3A Increases FAK Phosphorylation at Focal Adhesions to Modulate MDA-MB-231 Cell Migration and Spreading on Different Substratum Concentrations. Int J Breast Cancer. 2017;2017:9619734 pubmed publisher
Giacobini P, Parkash J, Campagne C, Messina A, Casoni F, Vanacker C, et al. Brain endothelial cells control fertility through ovarian-steroid-dependent release of semaphorin 3A. PLoS Biol. 2014;12:e1001808 pubmed publisher
Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold P, Menicanin D, et al. Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells. Stem Cells Dev. 2014;23:2225-36 pubmed publisher
van Gils J, Ramkhelawon B, Fernandes L, Stewart M, Guo L, Seibert T, et al. Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions. Arterioscler Thromb Vasc Biol. 2013;33:911-9 pubmed publisher
Chakraborty G, Kumar S, Mishra R, Patil T, Kundu G. Semaphorin 3A suppresses tumor growth and metastasis in mice melanoma model. PLoS ONE. 2012;7:e33633 pubmed publisher
Kabayama H, Takeuchi M, Taniguchi M, Tokushige N, Kozaki S, Mizutani A, et al. Syntaxin 1B suppresses macropinocytosis and semaphorin 3A-induced growth cone collapse. J Neurosci. 2011;31:7357-64 pubmed publisher
Tran Van H, Avota E, Börtlein C, Mueller N, Schneider Schaulies S. Measles virus modulates dendritic cell/T-cell communication at the level of plexinA1/neuropilin-1 recruitment and activity. Eur J Immunol. 2011;41:151-63 pubmed publisher
Verburg J, Hollenbeck P. Mitochondrial membrane potential in axons increases with local nerve growth factor or semaphorin signaling. J Neurosci. 2008;28:8306-15 pubmed publisher
Zacchigna S, Pattarini L, Zentilin L, Moimas S, Carrer A, Sinigaglia M, et al. Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice. J Clin Invest. 2008;118:2062-75 pubmed publisher
Marko S, Damon D. VEGF promotes vascular sympathetic innervation. Am J Physiol Heart Circ Physiol. 2008;294:H2646-52 pubmed publisher
Narazaki M, Segarra M, Tosato G. Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A. Blood. 2008;111:4126-36 pubmed publisher
Ohsaka A, Hirota Komatsu S, Shibata M, Ezaki J, Shinohara F, Yoshida T. Specific association of increased vascular endothelial growth factor expression and its receptors with macrophage differentiation of HL-60 leukemia cells. Biochem Biophys Res Commun. 2008;368:543-9 pubmed publisher
Acevedo L, Barillas S, Weis S, Gothert J, Cheresh D. Semaphorin 3A suppresses VEGF-mediated angiogenesis yet acts as a vascular permeability factor. Blood. 2008;111:2674-80 pubmed publisher
Jaalouk D, Ozawa M, Sun J, Lahdenranta J, Schlingemann R, Pasqualini R, et al. The original Pathologische Anatomie Leiden-Endothelium monoclonal antibody recognizes a vascular endothelial growth factor binding site within neuropilin-1. Cancer Res. 2007;67:9623-9 pubmed
Gallo G. RhoA-kinase coordinates F-actin organization and myosin II activity during semaphorin-3A-induced axon retraction. J Cell Sci. 2006;119:3413-23 pubmed
Wanigasekara Y, Keast J. Nerve growth factor, glial cell line-derived neurotrophic factor and neurturin prevent semaphorin 3A-mediated growth cone collapse in adult sensory neurons. Neuroscience. 2006;142:369-79 pubmed
Narazaki M, Tosato G. Ligand-induced internalization selects use of common receptor neuropilin-1 by VEGF165 and semaphorin3A. Blood. 2006;107:3892-901 pubmed

product information

master code :

1250-S3

SKU :

1250-S3-025

product name :

Recombinant Human Semaphorin 3A Fc Chimera Protein, CF

unit size :

25 ug

description :

The Recombinant Human Semaphorin 3A Fc Chimera Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Semaphorin 3A Fc Chimera Protein, CF has been validated for the following applications: Bioactivity.

target :

Semaphorin 3A

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in Tris, NaCl and Tween« 20.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

120 kDa, 90 kDa and 37 kDa, reducing conditions

theoretical molecular weight :

113.5 kDa (monomer)

gene symbol :

SEMA3A

details of functionality :

Measured by its binding ability in a functional ELISA. When Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1 ) is coated at 2 ╡g/mL, Recombinant Human Semaphorin 3A Fc Chimera (Catalog # 1250-S3) binds with an ED50 = 50-300 ng/mL. Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 20-60 ng/mL of Recombinant Human Semaphorin 3A Fc Chimera causes 50% growth cone collapse in the presence 10 ng/mL Recombinant Human ▀-NGF (Catalog # 256-GF ).

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

top caption :

Recombinant Human Semaphorin 3A Fc Chimera Protein Bioactivity

accessionNumbers :

Q14563

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived human Semaphorin 3A protein Met-6-His tag Human Semaphorin 3A Lys26-Val771 (Arg552Ala Arg555Ala) Accession # Q14563 DIEGRMD Human IgG1 (Pro100-Lys330) N-terminus C-terminus

source short :

NS0

USD :

436 USD

alt names :

(semaphorin) 3A, coll-1, collapsin 1, Hsema-I, Hsema-III, MGC133243, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, Sema III, SEMA1, Sema3A, SEMAD, SEMAIII, SEMAL, semaphorin D, Semaphorin III, semaphorin L, semaphorin-3A, SemD

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage