Recombinant Human Aggrecan G1-IGD-G2 Domains Protein, CF | R&D Systems 1220-PG-025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Aggrecan G1-IGD-G2 Domains Protein, CF

catalog :

1220-PG-025

quantity :

25 ug

price :

549 USD

citations: 7

Reference
L xe9 pine M, Douceau S, Devienne G, Prunotto P, Lenoir S, Regnauld C, et al. Parvalbumin interneuron-derived tissue-type plasminogen activator shapes perineuronal net structure. BMC Biol. 2022;20:218 pubmed publisher
Suzuki K, Kaseyama Takemoto H, Ito S. Highly sensitive quantification of bacterial chondroitin in a culture based on ELISA techniques. J Microbiol Methods. 2022;202:106579 pubmed publisher
Kim S, Song G, Shim A, Lee J, Eom C, Liu C, et al. Hyaluronan synthase 2, a target of miR-200c, promotes carbon tetrachloride-induced acute and chronic liver inflammation via regulation of CCL3 and CCL4. Exp Mol Med. 2022;54:739-752 pubmed publisher
Asega A, Menezes M, Trevisan Silva D, Cajado Carvalho D, Bertholim L, Oliveira A, et al. Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases. Sci Rep. 2020;10:12912 pubmed publisher
Wang P, Song X, Utpatel K, Shang R, Yang Y, Xu M, et al. MEK inhibition suppresses K-Ras wild-type cholangiocarcinoma in vitro and in vivo via inhibiting cell proliferation and modulating tumor microenvironment. Cell Death Dis. 2019;10:120 pubmed publisher
Wilkinson D, Desilets A, Lin H, Charlton S, Del Carmen Arques M, Falconer A, et al. The serine proteinase hepsin is an activator of pro-matrix metalloproteinases: molecular mechanisms and implications for extracellular matrix turnover. Sci Rep. 2017;7:16693 pubmed publisher
Chamberland A, Wang E, Jones A, Collins Racie L, LaVallie E, Huang Y, et al. Identification of a novel HtrA1-susceptible cleavage site in human aggrecan: evidence for the involvement of HtrA1 in aggrecan proteolysis in vivo. J Biol Chem. 2009;284:27352-9 pubmed publisher

product information

master code :

1220-PG

SKU :

1220-PG-025

product name :

Recombinant Human Aggrecan G1-IGD-G2 Domains Protein, CF

unit size :

25 ug

description :

The Recombinant Human Aggrecan G1-IGD-G2 Domains Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Aggrecan G1-IGD-G2 Domains Protein, CF has been validated for the following applications: Enzyme Activity, Binding Activity.

target :

Aggrecan

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in Tris and NaCl.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

120 kDa, reducing conditions

theoretical molecular weight :

74 kDa

gene symbol :

ACAN

details of functionality :

Can also be used as a protein substrate for proteases such as MMPs and ADAMTSs. The cleavage products can be analyzed by either SDS-PAGE or western blot using anti-human aggrecan antibodies (Catalog # AF1220 and MAB1220 ). Measured by its binding ability in a functional ELISA. Biotinylated hyaluronan immobilized on an EvenCoatTM Streptavidin Microplate (Catalog # CP003 ) at 0.1 ╡g/mL (100 ╡L/well) can bind recombinant human Aggrecan with a linear range of 8-500 ng/mL.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_037359

applications :

Enzyme Activity, Binding Activity

source long :

Mouse myeloma cell line, NS0-derived human Aggrecan protein Val20-Gly675, with a C-terminal 10-His tag

source short :

NS0

USD :

549 USD

alt names :

ACAN, AGC1, AGC1SEDK, aggrecan, aggrecan core protein, Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan 1, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPG1aggrecan 1, CSPGCP, large aggregating proteoglycan, MSK16, MSK16AGCAN, SEDK

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.