protein

rS. equi subsp. zooepidemicus IgG endopeptidase IdeZ, CF

11572-IZ-050

50 ug

423 USD

11572-IZ-050

rS. equi subsp. zooepidemicus IgG endopeptidase IdeZ, CF

50 ug

The rS. equi subsp. zooepidemicus IgG endopeptidase IdeZ, CF from R&D Systems is derived from E. coli. The rS. equi subsp. zooepidemicus IgG endopeptidase IdeZ, CF has been validated for the following applications: Enzyme Activity.

IdeZ

Proteins and Enzymes

Supplied as a 0.2 ╡m filtered solution in Tris and NaCl.

Unconjugated

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

S. equi subsp. Zooepidemicus

35-39 kDa, under reducing conditions

36 kDa

Measured by its ability to cleave Human IgG. The DC50 is 25 ng, as measured under the described conditions. The DC50 is defined as the amount of enzyme required to cleave 50% of 1 ╡g human IgG in 30 minutes at 37 ░C. Use of Recombinant Streptococcus equi subsp. zooepidemicus IdeZ in the cleavage of other IgGs may required alternative conditions for optimal performance.

<0.10 EU per 1 ╡g of the protein by the LAL method.

WP_014622780.1

Enzyme Activity

E. coli -derived IdeZ protein Asp35-Ser349, with an N-terminal Met and C-terminal 6-His tag

E. coli

Recombinant Streptococcus His-tag

423 USD

Recombinant Streptococcus His-tag

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.