protein

Recombinant Human 5'-Nucleotidase/CD73 Fc Protein, CF

11415-EN-025

25 ug

509 USD

11415-EN-025

Recombinant Human 5'-Nucleotidase/CD73 Fc Protein, CF

25 ug

The Recombinant Human 5'-Nucleotidase/CD73 Fc Protein, CF from R&D Systems is derived from HEK293. The Recombinant Human 5'-Nucleotidase/CD73 Fc Protein, CF has been validated for the following applications: Enzyme Activity.

5'-Nucleotidase/CD73

Proteins and Enzymes

Supplied as a 0.2 ╡m filtered solution in Tris, NaCl, CaCl2 and Glycerol.

Unconjugated

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Human

84-93 kDa under reducing conditions.

85 kDa

Measured by its ability to hydrolyze the 5Æ-phosphate group from the substrate adenosine-5Æ-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996 ). The specific activity is 12,500 pmol/min/╡g, as measured under the described conditions.

<0.10 EU per 1 ╡g of the protein by the LAL method.

AAH65937.1

Enzyme Activity

Human embryonic kidney cell, HEK293-derived human 5'-Nucleotidase/CD73 protein Human CD73 (Trp27-Ser549) Accession # AAH65937.1 GGIEGRMD Mouse IgG2a (Pro100-Lys330) N-terminus C-terminus

HEK293

Fc Chimera

509 USD

Fc Chimera

5' nucleotidase (CD73), 5'-NT, 5-NT, 5'-nucleotidase, ecto (CD73), CD73, CD73 antigen, E5NT, EC 3.1.3.5, ecto-5'-nucleotidase, eN, eNT, NT, NT55'-nucleotidase, NT5E, NTE, Purine 5-Prime-Nucleotidase

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.