product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
catalog :
1129-ER-050
quantity :
50 ug (also 1 mg)
price :
432 USD
more info or order :
citations: 58
Reference
Sakata J, Tatsumi T, Sugiyama A, Shimizu A, Inagaki Y, Katoh H, et al. Antibody-mimetic drug conjugate with efficient internalization activity using anti-HER2 VHH and duocarmycin. Protein Expr Purif. 2024;214:106375 pubmed publisher
Park J, Cho Y, Chae J, Lee J, Kang W. Enhancement of in vivo targeting properties of ErbB2 aptamer by chemical modification. PLoS ONE. 2023;18:e0291624 pubmed publisher
Mez x151 si Csapl xe1 r M, Sz xf6 x151 r x, Vereb G. CD28 and 41BB Costimulatory Domains Alone or in Combination Differentially Influence Cell Surface Dynamics and Organization of Chimeric Antigen Receptors and Early Activation of CAR T Cells. Cancers (Basel). 2023;15: pubmed publisher
Po x17a niak M, x17b ukowska D, G x119 daj A, Krzy x15b cik M, Por x119 bska N, Zakrzewska M, et al. The Significance of Cell Surface N-Glycosylation for Internalization and Potency of Cytotoxic Conjugates Targeting Receptor Tyrosine Kinases. Int J Mol Sci. 2022;23: pubmed publisher
Hebbar N, Epperly R, Vaidya A, Thanekar U, Moore S, Umeda M, et al. CAR T cells redirected to cell surface GRP78 display robust anti-acute myeloid leukemia activity and do not target hematopoietic progenitor cells. Nat Commun. 2022;13:587 pubmed publisher
Garousi J, Ding H, von Witting E, Xu T, Vorobyeva A, Oroujeni M, et al. Targeting HER2 Expressing Tumors with a Potent Drug Conjugate Based on an Albumin Binding Domain-Derived Affinity Protein. Pharmaceutics. 2021;13: pubmed publisher
Stoup N, Liberelle M, Schulz C, Cavdarli S, Vasseur R, Magnez R, et al. The EGF Domains of MUC4 Oncomucin Mediate HER2 Binding Affinity and Promote Pancreatic Cancer Cell Tumorigenesis. Cancers (Basel). 2021;13: pubmed publisher
Chidiac R, Abedin M, Macleod G, Yang A, Thibeault P, Blazer L, et al. A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy. EMBO Mol Med. 2021;13:e13977 pubmed publisher
Ding H, Xu T, Zhang J, Tolmachev V, Oroujeni M, Orlova A, et al. Affibody-Derived Drug Conjugates Targeting HER2: Effect of Drug Load on Cytotoxicity and Biodistribution. Pharmaceutics. 2021;13: pubmed publisher
Yuan S, Brooks S, Nguyen A, Lin W, Johnston T, Maynard J, et al. Bioproduced Proteins On Demand (Bio-POD) in hydrogels using Pichia pastoris. Bioact Mater. 2021;6:2390-2399 pubmed publisher
Xu T, Ding H, Vorobyeva A, Oroujeni M, Orlova A, Tolmachev V, et al. Drug Conjugates Based on a Monovalent Affibody Targeting Vector Can Efficiently Eradicate HER2 Positive Human Tumors in an Experimental Mouse Model. Cancers (Basel). 2020;13: pubmed publisher
Youness R, Gad A, Sanber K, Ahn Y, Lee G, Khallaf E, et al. Targeting hydrogen sulphide signaling in breast cancer. J Adv Res. 2021;27:177-190 pubmed publisher
Dengl S, Mayer K, Bormann F, Duerr H, Hoffmann E, Nussbaum B, et al. Format chain exchange (FORCE) for high-throughput generation of bispecific antibodies in combinatorial binder-format matrices. Nat Commun. 2020;11:4974 pubmed publisher
Kondo T, Iwatani Y, Matsuoka K, Fujino T, Umemoto S, Yokomaku Y, et al. Antibody-like proteins that capture and neutralize SARS-CoV-2. Sci Adv. 2020;6: pubmed publisher
Wege A, Kirchhammer N, Kazandjian L, Prassl S, Brandt M, Piendl G, et al. A novel rabbit derived anti-HER2 antibody with pronounced therapeutic effectiveness on HER2-positive breast cancer cells in vitro and in humanized tumor mice (HTM). J Transl Med. 2020;18:316 pubmed publisher
Hegde M, Joseph S, Pashankar F, Derenzo C, Sanber K, Navai S, et al. Tumor response and endogenous immune reactivity after administration of HER2 CAR T cells in a child with metastatic rhabdomyosarcoma. Nat Commun. 2020;11:3549 pubmed publisher
Dai Z, Zhang X, Nasertorabi F, Cheng Q, Li J, Katz B, et al. Synthesis of site-specific antibody-drug conjugates by ADP-ribosyl cyclases. Sci Adv. 2020;6:eaba6752 pubmed publisher
Crauwels M, Van Vaerenbergh N, Kulaya N, Vincke C, D Huyvetter M, Devoogdt N, et al. Reshaping nanobodies for affinity purification on protein a. N Biotechnol. 2020;57:20-28 pubmed publisher
Kadonosono T, Yimchuen W, Ota Y, See K, Furuta T, Shiozawa T, et al. Design Strategy to Create Antibody Mimetics Harbouring Immobilised Complementarity Determining Region Peptides for Practical Use. Sci Rep. 2020;10:891 pubmed publisher
Parker C, Jacobs T, Huckaby J, Harit D, Lai S. Efficient and Highly Specific Gene Transfer Using Mutated Lentiviral Vectors Redirected with Bispecific Antibodies. MBio. 2020;11: pubmed publisher
Liberelle M, Magnez R, Thuru X, Bencheikh Y, Ravez S, Quenon C, et al. MUC4-ErbB2 Oncogenic Complex: Binding studies using Microscale Thermophoresis. Sci Rep. 2019;9:16678 pubmed publisher
Medina Echeverz J, Hinterberger M, Testori M, Geiger M, Giessel R, Bathke B, et al. Synergistic cancer immunotherapy combines MVA-CD40L induced innate and adaptive immunity with tumor targeting antibodies. Nat Commun. 2019;10:5041 pubmed publisher
Ahn S, Li J, Sun C, Gao K, Hirabayashi K, Li H, et al. Cancer Immunotherapy with T Cells Carrying Bispecific Receptors That Mimic Antibodies. Cancer Immunol Res. 2019;7:773-783 pubmed publisher
Tobin S, Wakefield D, Jones V, Liu X, Schmolze D, Jovanovic Talisman T. Single molecule localization microscopy coupled with touch preparation for the quantification of trastuzumab-bound HER2. Sci Rep. 2018;8:15154 pubmed publisher
Helsen C, Hammill J, Lau V, Mwawasi K, Afsahi A, Bezverbnaya K, et al. The chimeric TAC receptor co-opts the T cell receptor yielding robust anti-tumor activity without toxicity. Nat Commun. 2018;9:3049 pubmed publisher
Pruszyński M, Kang C, Koumarianou E, Vaidyanathan G, Zalutsky M. d-Amino Acid Peptide Residualizing Agents for Protein Radioiodination: Effect of Aspartate for Glutamate Substitution. Molecules. 2018;23: pubmed publisher
Kahl M, Settele F, Knick P, Haupts U, Bosse Doenecke E. Mabfilin and Fabfilin - New antibody-scaffold fusion formats for multispecific targeting concepts. Protein Expr Purif. 2018;149:51-65 pubmed publisher
Tolmachev V, Grönroos T, Yim C, Garousi J, Yue Y, Grimm S, et al. Molecular design of radiocopper-labelled Affibody molecules. Sci Rep. 2018;8:6542 pubmed publisher
Correa I, Ilieva K, Crescioli S, Lombardi S, Figini M, Cheung A, et al. Evaluation of Antigen-Conjugated Fluorescent Beads to Identify Antigen-Specific B Cells. Front Immunol. 2018;9:493 pubmed publisher
Nguyen A, Le K, Maynard J. Identification of high affinity HER2 binding antibodies using CHO Fab surface display. Protein Eng Des Sel. 2018;31:91-101 pubmed publisher
Lee J, Kang H, Bae J, Kim K, Lee K, Lim K, et al. Evaluation of analytical similarity between trastuzumab biosimilar CT-P6 and reference product using statistical analyses. MAbs. 2018;10:547-571 pubmed publisher
Bielamowicz K, Fousek K, Byrd T, Samaha H, Mukherjee M, Aware N, et al. Trivalent CAR T-cells Overcome Interpatient Antigenic Variability in Glioblastoma. Neuro Oncol. 2017;: pubmed publisher
Yun M, Kim D, Lee J, Kim H, Kim H, Pyo A, et al. A High-Affinity Repebody for Molecular Imaging of EGFR-Expressing Malignant Tumors. Theranostics. 2017;7:2620-2633 pubmed publisher
Shinozaki N, Hashimoto R, Fukui K, Uchiyama S. Efficient generation of single domain antibodies with high affinities and enhanced thermal stabilities. Sci Rep. 2017;7:5794 pubmed publisher
Petrović B, Gianni T, Gatta V, Campadelli Fiume G. Insertion of a ligand to HER2 in gB retargets HSV tropism and obviates the need for activation of the other entry glycoproteins. PLoS Pathog. 2017;13:e1006352 pubmed publisher
Tobias J, Jasinska J, Baier K, Kundi M, Ede N, Zielinski C, et al. Enhanced and long term immunogenicity of a Her-2/neu multi-epitope vaccine conjugated to the carrier CRM197 in conjunction with the adjuvant Montanide. BMC Cancer. 2017;17:118 pubmed publisher
Leung I, Jarvik N, Sidhu S. A Highly Diverse and Functional Naïve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents. J Mol Biol. 2017;429:115-127 pubmed publisher
Dai P, Zhang C, Welborn M, Shepherd J, Zhu T, Van Voorhis T, et al. Salt Effect Accelerates Site-Selective Cysteine Bioconjugation. ACS Cent Sci. 2016;2:637-646 pubmed
Hegde M, Mukherjee M, Grada Z, Pignata A, Landi D, Navai S, et al. Tandem CAR T cells targeting HER2 and IL13R?2 mitigate tumor antigen escape. J Clin Invest. 2016;126:3036-52 pubmed publisher
Selis F, Focà G, Sandomenico A, Marra C, Di Mauro C, Saccani Jotti G, et al. Pegylated Trastuzumab Fragments Acquire an Increased in Vivo Stability but Show a Largely Reduced Affinity for the Target Antigen. Int J Mol Sci. 2016;17:491 pubmed publisher
Kanojia D, Balyasnikova I, Morshed R, Frank R, Yu D, Zhang L, et al. Neural Stem Cells Secreting Anti-HER2 Antibody Improve Survival in a Preclinical Model of HER2 Overexpressing Breast Cancer Brain Metastases. Stem Cells. 2015;33:2985-94 pubmed publisher
Leung K, Batey S, Rowlands R, Isaac S, Jones P, Drewett V, et al. A HER2-specific Modified Fc Fragment (Fcab) Induces Antitumor Effects Through Degradation of HER2 and Apoptosis. Mol Ther. 2015;23:1722-1733 pubmed publisher
Zhou Q, Uhlig K, Muth A, Kimpel J, Lévy C, Münch R, et al. Exclusive Transduction of Human CD4+ T Cells upon Systemic Delivery of CD4-Targeted Lentiviral Vectors. J Immunol. 2015;195:2493-501 pubmed publisher
Vanseggelen H, Hammill J, Dvorkin Gheva A, Tantalo D, Kwiecien J, Denisova G, et al. T Cells Engineered With Chimeric Antigen Receptors Targeting NKG2D Ligands Display Lethal Toxicity in Mice. Mol Ther. 2015;23:1600-10 pubmed publisher
Lin C, Pan I, Li Y, Pan Y, Lin M, Lu Y, et al. The adjuvant effects of high-molecule-weight polysaccharides purified from Antrodia cinnamomea on dendritic cell function and DNA vaccines. PLoS ONE. 2015;10:e0116191 pubmed publisher
Liu Z, Leng E, Gunasekaran K, Pentony M, Shen M, Howard M, et al. A novel antibody engineering strategy for making monovalent bispecific heterodimeric IgG antibodies by electrostatic steering mechanism. J Biol Chem. 2015;290:7535-62 pubmed publisher
Schönfeld K, Sahm C, Zhang C, Naundorf S, Brendel C, Odendahl M, et al. Selective inhibition of tumor growth by clonal NK cells expressing an ErbB2/HER2-specific chimeric antigen receptor. Mol Ther. 2015;23:330-8 pubmed publisher
Nilvebrant J, Astrand M, Georgieva Kotseva M, Björnmalm M, Löfblom J, Hober S. Engineering of bispecific affinity proteins with high affinity for ERBB2 and adaptable binding to albumin. PLoS ONE. 2014;9:e103094 pubmed publisher
Hussain S, Rodriguez Fernandez M, Braun G, Doyle F, Ruoslahti E. Quantity and accessibility for specific targeting of receptors in tumours. Sci Rep. 2014;4:5232 pubmed publisher
Gu J, Yang J, Chang Q, Lu X, Wang J, Chen M, et al. Identification of anti-ErbB2 dual variable domain immunoglobulin (DVD-Ig™) proteins with unique activities. PLoS ONE. 2014;9:e97292 pubmed publisher
Kang J, Poovassery J, Bansal P, You S, Manjarres I, Ober R, et al. Engineering multivalent antibodies to target heregulin-induced HER3 signaling in breast cancer cells. MAbs. 2014;6:340-53 pubmed publisher
Hegde M, Corder A, Chow K, Mukherjee M, Ashoori A, Kew Y, et al. Combinational targeting offsets antigen escape and enhances effector functions of adoptively transferred T cells in glioblastoma. Mol Ther. 2013;21:2087-101 pubmed publisher
Frese K, Eisenmann M, Ostendorp R, Brocks B, Pabst S. An automated immunoassay for early specificity profiling of antibodies. MAbs. 2013;5:279-87 pubmed publisher
Axup J, Bajjuri K, Ritland M, Hutchins B, Kim C, Kazane S, et al. Synthesis of site-specific antibody-drug conjugates using unnatural amino acids. Proc Natl Acad Sci U S A. 2012;109:16101-6 pubmed publisher
Feldwisch J, Tolmachev V, Lendel C, Herne N, Sjöberg A, Larsson B, et al. Design of an optimized scaffold for affibody molecules. J Mol Biol. 2010;398:232-47 pubmed publisher
Serpe M, Umulis D, Ralston A, Chen J, Olson D, Avanesov A, et al. The BMP-binding protein Crossveinless 2 is a short-range, concentration-dependent, biphasic modulator of BMP signaling in Drosophila. Dev Cell. 2008;14:940-53 pubmed publisher
Gonzalez R, Seurynck Servoss S, Crowley S, Brown M, Omenn G, Hayes D, et al. Development and validation of sandwich ELISA microarrays with minimal assay interference. J Proteome Res. 2008;7:2406-14 pubmed publisher
Ahmed N, Ratnayake M, Savoldo B, Perlaky L, Dotti G, Wels W, et al. Regression of experimental medulloblastoma following transfer of HER2-specific T cells. Cancer Res. 2007;67:5957-64 pubmed
product information
brand :
R&D Systems
master code :
1129-ER
SKU :
1129-ER-050
product name :
Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
unit size :
50 ug (also 1 mg)
seo description :
The Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF from R&D Systems is derived from NS0. The Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF has been validated for the following applications: Bioactivity.
target :
ErbB2/Her2
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS.
conjugate :
Unconjugated
dilution :
Bioactivity
purity :
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Human
observed molecular weight :
125-130 kDa, reducing conditions 220-250 kDa, non-reducing conditions
theoretical molecular weight :
96.8 kDa (monomer)
gene symbol :
ERBB2
details of functionality :
Measured by its ability to block anti-ErbB2 mediated inhibition of SK-BR-3 human breast cancer cell proliferation. Brodowicz, T. et al . (1997) Int. J. Cancer 73 :875. The ED50 for this effect is 0.3-1.2 ╡g/mL in the presence of 0.6 ╡g/mL Anti-Human ErbB2/Her2 Monoclonal Antibody (Trastuzumab, Catalog # MAB9589 ).
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
top caption :
Graph showing dose-dependent bioactivity of human ErbB2/ Her2 protein
accessionNumbers :
NP_004439
applications :
Bioactivity
source :
NS0-derived Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF
USD :
432 USD
alt names :
CD340, CD340 antigen, c-erb B2/neu protein, EC 2.7.10, EGFR2, HER2, HER-2, HER2EC 2.7.10.1, herstatin, Metastatic lymph node gene 19 protein, MLN 19, MLN19, Neu Oncogene, NEUHER-2/neu, neuroblastoma/glioblastoma derived oncogene homolog, NGL, NGLTKR1, p185erbB2, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase-type cell surface receptor HER2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog), v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.