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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human PD-1 Fc Chimera Protein, CF
catalog :
1086-PD-050
quantity :
50 ug (also 1 mg)
price :
425 USD
more info or order :
citations: 39
Reference
Huang H, Huang Y, Chen Y, Wu H, Hsu C, Kao H, et al. The branched N-glycan of PD-L1 predicts immunotherapy responses in patients with recurrent/metastatic HNSCC. Oncogenesis. 2024;13:36 pubmed publisher
Robinson M, Kennedy A, Orozco C, Chen H, Waters E, Giovacchini D, et al. Rigid, bivalent CTLA-4 binding to CD80 is required to disrupt the cis CD80/PD-L1 interaction. Cell Rep. 2024;43:114768 pubmed publisher
Pereira Ribeiro S, Strongin Z, Soudeyns H, Ten Caten F, Ghneim K, Pacheco Sanchez G, et al. Dual blockade of IL-10 and PD-1 leads to control of SIV viral rebound following analytical treatment interruption. Nat Immunol. 2024;25:1900-1912 pubmed publisher
Li Y, Wang Z, Gao P, Cao D, Dong R, Zhu M, et al. CircRHBDD1 promotes immune escape via IGF2BP2/PD-L1 signaling and acts as a nanotherapeutic target in gastric cancer. J Transl Med. 2024;22:704 pubmed publisher
Jeong H, Koh J, Kim S, Song S, Lee S, Jeon Y, et al. Epithelial-mesenchymal transition induced by tumor cell-intrinsic PD-L1 signaling predicts a poor response to immune checkpoint inhibitors in PD-L1-high lung cancer. Br J Cancer. 2024;131:23-36 pubmed publisher
Manna L, Rapuano Lembo R, Yoshioka A, Nakamura K, Passariello M, De Lorenzo C. A Comparison of the Antitumor Efficacy of Novel Multi-Specific Tribodies with Combinations of Approved Immunomodulatory Antibodies. Cancers (Basel). 2023;15: pubmed publisher
Erlichman N, Meshel T, Baram T, Abu Raiya A, Horvitz T, Ben Yaakov H, et al. The Cell-Autonomous Pro-Metastatic Activities of PD-L1 in Breast Cancer Are Regulated by N-Linked Glycosylation-Dependent Activation of STAT3 and STAT1. Cells. 2023;12: pubmed publisher
Mellergaard M, Skovbakke S, Jepsen S, Panagiotopoulou N, Hansen A, Tian W, et al. Clinical Staphylococcus aureus inhibits human T-cell activity through interaction with the PD-1 receptor. MBio. 2023;14:e0134923 pubmed publisher
Li X, Li J, Zheng Y, Lee S, Zhou J, Giobbie Hurder A, et al. Granulocyte-Macrophage Colony-Stimulating Factor Influence on Soluble and Membrane-Bound ICOS in Combination with Immune Checkpoint Blockade. Cancer Immunol Res. 2023;11:1100-1113 pubmed publisher
Phakham T, Boonkrai C, Wongtangprasert T, Audomsun T, Attakitbancha C, Saelao P, et al. Highly efficient hybridoma generation and screening strategy for anti-PD-1 monoclonal antibody development. Sci Rep. 2022;12:17792 pubmed publisher
Wang Q, Cao Y, Shen L, Xiao T, Cao R, Wei S, et al. Regulation of PD-L1 through direct binding of cholesterol to CRAC motifs. Sci Adv. 2022;8:eabq4722 pubmed publisher
Ghaderi S, Riazi Rad F, Qamsari E, Bagheri S, Rahimi Jamnani F, Sharifzadeh Z. Development of a human phage display-derived anti-PD-1 scFv antibody: an attractive tool for immune checkpoint therapy. BMC Biotechnol. 2022;22:22 pubmed publisher
Erlichman N, Baram T, Meshel T, Morein D, Da adoosh B, Ben Baruch A. Tumor Cell-Autonomous Pro-Metastatic Activities of PD-L1 in Human Breast Cancer Are Mediated by PD-L1-S283 and Chemokine Axes. Cancers (Basel). 2022;14: pubmed publisher
Zhang R, Yang Y, Dong W, Lin M, He J, Zhang X, et al. D-mannose facilitates immunotherapy and radiotherapy of triple-negative breast cancer via degradation of PD-L1. Proc Natl Acad Sci U S A. 2022;119: pubmed publisher
Edwards C, Sette A, Cox C, Di Fiore B, Wyre C, Sydoruk D, et al. The multi-specific VH-based Humabody CB213 co-targets PD1 and LAG3 on T cells to promote anti-tumour activity. Br J Cancer. 2021;: pubmed publisher
Ren Y, Qian Y, Ai L, Xie Y, Gao Y, Zhuang Z, et al. TRAPPC4 regulates the intracellular trafficking of PD-L1 and antitumor immunity. Nat Commun. 2021;12:5405 pubmed publisher
Vetrei C, Passariello M, Froechlich G, Rapuano Lembo R, Zambrano N, De Lorenzo C. Immunomodulatory mAbs as Tools to Investigate on Cis-Interaction of PD-1/PD-L1 on Tumor Cells and to Set Up Methods for Early Screening of Safe and Potent Combinatorial Treatments. Cancers (Basel). 2021;13: pubmed publisher
Park J, Thi E, Carpio V, Bi Y, Cole A, Dorsey B, et al. Checkpoint inhibition through small molecule-induced internalization of programmed death-ligand 1. Nat Commun. 2021;12:1222 pubmed publisher
Zhu D, Xu R, Huang X, Tang Z, Tian Y, Zhang J, et al. Deubiquitinating enzyme OTUB1 promotes cancer cell immunosuppression via preventing ER-associated degradation of immune checkpoint protein PD-L1. Cell Death Differ. 2021;28:1773-1789 pubmed publisher
Li C, Yao H, Wang H, Fang J, Xu J. Repurposing screen identifies Amlodipine as an inducer of PD-L1 degradation and antitumor immunity. Oncogene. 2021;40:1128-1146 pubmed publisher
Silva Pilipich N, Martisova E, Ballesteros Briones M, Hervas Stubbs S, Casares N, Gonzalez Sapienza G, et al. Long-Term Systemic Expression of a Novel PD-1 Blocking Nanobody from an AAV Vector Provides Antitumor Activity without Toxicity. Biomedicines. 2020;8: pubmed publisher
Liberelle M, Magnez R, Thuru X, Bencheikh Y, Ravez S, Quenon C, et al. MUC4-ErbB2 Oncogenic Complex: Binding studies using Microscale Thermophoresis. Sci Rep. 2019;9:16678 pubmed publisher
Passariello M, D Alise A, Esposito A, Vetrei C, Froechlich G, Scarselli E, et al. Novel Human Anti-PD-L1 mAbs Inhibit Immune-Independent Tumor Cell Growth and PD-L1 Associated Intracellular Signalling. Sci Rep. 2019;9:13125 pubmed publisher
Wang W, Chapman N, Zhang B, Li M, Fan M, Laribee R, et al. Upregulation of PD-L1 via HMGB1-Activated IRF3 and NF-κB Contributes to UV Radiation-Induced Immune Suppression. Cancer Res. 2019;79:2909-2922 pubmed publisher
Zhang N, Dou Y, Liu L, Zhang X, Liu X, Zeng Q, et al. SA-49, a novel aloperine derivative, induces MITF-dependent lysosomal degradation of PD-L1. EBioMedicine. 2019;40:151-162 pubmed publisher
Wang H, Yao H, Li C, Shi H, Lan J, Li Z, et al. HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity. Nat Chem Biol. 2019;15:42-50 pubmed publisher
Shao B, Li C, Lim S, Sun L, Lai Y, Hou J, et al. Deglycosylation of PD-L1 by 2-deoxyglucose reverses PARP inhibitor-induced immunosuppression in triple-negative breast cancer. Am J Cancer Res. 2018;8:1837-1846 pubmed
Osa A, Uenami T, Koyama S, Fujimoto K, Okuzaki D, Takimoto T, et al. Clinical implications of monitoring nivolumab immunokinetics in non-small cell lung cancer patients. JCI Insight. 2018;3: pubmed publisher
Li C, Lim S, Chung E, Kim Y, Park A, Yao J, et al. Eradication of Triple-Negative Breast Cancer Cells by Targeting Glycosylated PD-L1. Cancer Cell. 2018;33:187-201.e10 pubmed publisher
Chatterjee S, Lesniak W, Miller M, Lisok A, Sikorska E, Wharram B, et al. Rapid PD-L1 detection in tumors with PET using a highly specific peptide. Biochem Biophys Res Commun. 2017;483:258-263 pubmed publisher
Li C, Lim S, Xia W, Lee H, Chan L, Kuo C, et al. Glycosylation and stabilization of programmed death ligand-1 suppresses T-cell activity. Nat Commun. 2016;7:12632 pubmed publisher
Kataoka K, Shiraishi Y, Takeda Y, Sakata S, Matsumoto M, Nagano S, et al. Aberrant PD-L1 expression through 3'-UTR disruption in multiple cancers. Nature. 2016;534:402-6 pubmed publisher
Yang Y, Wu K, Zhao E, Li W, Shi L, Xie G, et al. B7-H1 enhances proliferation ability of gastric cancer stem-like cells as a receptor. Oncol Lett. 2015;9:1833-1838 pubmed
Melendreras S, Martínez Camblor P, Menéndez A, Bravo Mendoza C, González Vidal A, Coto E, et al. Soluble co-signaling molecules predict long-term graft outcome in kidney-transplanted patients. PLoS ONE. 2014;9:e113396 pubmed publisher
Steidl C, Shah S, Woolcock B, Rui L, Kawahara M, Farinha P, et al. MHC class II transactivator CIITA is a recurrent gene fusion partner in lymphoid cancers. Nature. 2011;471:377-81 pubmed publisher
Brahmer J, Drake C, Wollner I, Powderly J, Picus J, Sharfman W, et al. Phase I study of single-agent anti-programmed death-1 (MDX-1106) in refractory solid tumors: safety, clinical activity, pharmacodynamics, and immunologic correlates. J Clin Oncol. 2010;28:3167-75 pubmed publisher
Kim Y, Park G, Lee H, Song H, Choi I, Lee W, et al. Cross-linking of B7-H1 on EBV-transformed B cells induces apoptosis through reactive oxygen species production, JNK signaling activation, and fasL expression. J Immunol. 2008;181:6158-69 pubmed
Sakthivel P, Ramanujam R, Wang X, Pirskanen R, Lefvert A. Programmed Death-1: from gene to protein in autoimmune human myasthenia gravis. J Neuroimmunol. 2008;193:149-55 pubmed
Wan B, Nie H, Liu A, Feng G, He D, Xu R, et al. Aberrant regulation of synovial T cell activation by soluble costimulatory molecules in rheumatoid arthritis. J Immunol. 2006;177:8844-50 pubmed
product information
brand :
R&D Systems
master code :
1086-PD
SKU :
1086-PD-050
product name :
Recombinant Human PD-1 Fc Chimera Protein, CF
unit size :
50 ug (also 1 mg)
seo description :
The Recombinant Human PD-1 Fc Chimera Protein, CF from R&D Systems is derived from NS0. The Recombinant Human PD-1 Fc Chimera Protein, CF has been validated for the following applications: Binding Activity.
target :
PD-1
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS.
conjugate :
Unconjugated
dilution :
Binding Activity
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
Human
observed molecular weight :
60 - 70 kDa, under reducing conditions.
theoretical molecular weight :
42.6 kDa (monomer)
gene symbol :
PDCD1
details of functionality :
Measured by its binding ability in a functional ELISA. When Recombinant Human PD-1 Fc Chimera is immobilized at 0.1 ╡g/mL (100 ╡L/well), Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7 ) binds with a typical ED50 of 0.15-0.75 ╡g/mL.
endotoxin note :
<0.01 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
Q15116.3
applications :
Binding Activity
source :
NS0-derived Recombinant Human PD-1 Fc Chimera Protein, CF
extended description :
Analyzed by SEC-MALS
USD :
425 USD
product details :
Analyzed by SEC-MALS
alt names :
CD279, CD279 antigen, hPD-1, PD1, PD1hPD-l, PDCD1, programmed cell death 1, programmed cell death protein 1, Protein PD-1, SLEB2
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.