Recombinant Human HAI-1 Protein, CF | R&D Systems 1048-PI product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human HAI-1 Protein, CF

catalog :

1048-PI

quantity :

10 ug

price :

339 USD

citations: 6

Reference
Szabo R, Bugge T. Loss of HAI-2 in mice with decreased prostasin activity leads to an early-onset intestinal failure resembling congenital tufting enteropathy. PLoS ONE. 2018;13:e0194660 pubmed publisher
Murray A, Varela F, Hyland T, Schoenbeck A, White J, Tanabe L, et al. Phosphorylation of the type II transmembrane serine protease, TMPRSS13, in hepatocyte growth factor activator inhibitor-1 and -2-mediated cell-surface localization. J Biol Chem. 2017;292:14867-14884 pubmed publisher
Sales K, Masedunskas A, Bey A, Rasmussen A, Weigert R, List K, et al. Matriptase initiates activation of epidermal pro-kallikrein and disease onset in a mouse model of Netherton syndrome. Nat Genet. 2010;42:676-83 pubmed publisher
Szabo R, Hobson J, List K, Molinolo A, Lin C, Bugge T. Potent inhibition and global co-localization implicate the transmembrane Kunitz-type serine protease inhibitor hepatocyte growth factor activator inhibitor-2 in the regulation of epithelial matriptase activity. J Biol Chem. 2008;283:29495-504 pubmed publisher
Butler G, Dean R, Tam E, Overall C. Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding. Mol Cell Biol. 2008;28:4896-914 pubmed publisher
Cortesio C, Jiang W. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Arch Biochem Biophys. 2006;449:164-70 pubmed

product information

brand :

R&D Systems

master code :

1048-PI

SKU :

1048-PI-010

product name :

Recombinant Human HAI-1 Protein, CF

description :

The Recombinant Human HAI-1 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human HAI-1 Protein, CF has been validated for the following applications: Inhibition Activity.

target :

HAI-1/HGFA Inhibitor 1

category :

Proteins and Enzymes

unit size :

10 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

observed molecular weight :

58 kDa, reducing conditions

theoretical molecular weight :

47 kDa

gene symbol :

SPINT1

details of functionality :

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002 ). The IC50 is 2 nM, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 µg of the protein by the LAL method.

catalog number base :

1048-PI

accessionNumbers :

NP_003701

applications :

Inhibition Activity

source :

Mouse myeloma cell line, NS0-derived human HAI-1 protein Pro37-Glu449, with a C-terminal 10-His tag

2020 USD :

339

2021 USD :

339 USD

alt names :

HAI, HAI1, HAI-1, hepatocyte growth factor activator inhibitor 1, Hepatocyte growth factor activator inhibitor type 1, kunitz-type protease inhibitor 1, MANSC2, serine peptidase inhibitor, Kunitz type 1, serine protease inhibitor, Kunitz type 1, SPINT1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.