Recombinant Human HAI-1 Protein, CF | R&D Systems 1048-PI-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human HAI-1 Protein, CF

catalog :

1048-PI-010

quantity :

10 ug

price :

432 USD

more info or order :

R&D Systems product webpage

citations: 6

Reference
Szabo R, Bugge T. Loss of HAI-2 in mice with decreased prostasin activity leads to an early-onset intestinal failure resembling congenital tufting enteropathy. PLoS ONE. 2018;13:e0194660 pubmed publisher
Murray A, Varela F, Hyland T, Schoenbeck A, White J, Tanabe L, et al. Phosphorylation of the type II transmembrane serine protease, TMPRSS13, in hepatocyte growth factor activator inhibitor-1 and -2-mediated cell-surface localization. J Biol Chem. 2017;292:14867-14884 pubmed publisher
Sales K, Masedunskas A, Bey A, Rasmussen A, Weigert R, List K, et al. Matriptase initiates activation of epidermal pro-kallikrein and disease onset in a mouse model of Netherton syndrome. Nat Genet. 2010;42:676-83 pubmed publisher
Szabo R, Hobson J, List K, Molinolo A, Lin C, Bugge T. Potent inhibition and global co-localization implicate the transmembrane Kunitz-type serine protease inhibitor hepatocyte growth factor activator inhibitor-2 in the regulation of epithelial matriptase activity. J Biol Chem. 2008;283:29495-504 pubmed publisher
Butler G, Dean R, Tam E, Overall C. Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding. Mol Cell Biol. 2008;28:4896-914 pubmed publisher
Cortesio C, Jiang W. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Arch Biochem Biophys. 2006;449:164-70 pubmed

product information

master code :

1048-PI

SKU :

1048-PI-010

product name :

Recombinant Human HAI-1 Protein, CF

unit size :

10 ug

description :

The Recombinant Human HAI-1 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human HAI-1 Protein, CF has been validated for the following applications: Inhibition Activity.

target :

HAI-1

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in Tris and NaCl.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

58 kDa, reducing conditions

theoretical molecular weight :

47 kDa

gene symbol :

SPINT1

details of functionality :

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002 ). The IC50 is 2 nM, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_003701

applications :

Inhibition Activity

source long :

Mouse myeloma cell line, NS0-derived human HAI-1 protein Pro37-Glu449, with a C-terminal 10-His tag

source short :

NS0

USD :

432 USD

alt names :

HAI, HAI1, HAI-1, hepatocyte growth factor activator inhibitor 1, Hepatocyte growth factor activator inhibitor type 1, kunitz-type protease inhibitor 1, MANSC2, serine peptidase inhibitor, Kunitz type 1, serine protease inhibitor, Kunitz type 1, SPINT1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage