product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant E. coli BirA His-tag Protein, CF
catalog :
10464-BA-100
quantity :
100 ug
price :
425 USD
more info or order :
R&D Systems product webpage
citations: 2
Reference
Liu S, Tong B, Mason J, Ostrem J, TUTTER A, Hua B, et al. Rational Screening for Cooperativity in Small-Molecule Inducers of Protein-Protein Associations. J Am Chem Soc. 2023;145:23281-23291 pubmed publisher
Liu S, Tong B, Mason J, Ostrem J, TUTTER A, Hua B, et al. Rational screening for cooperativity in small-molecule inducers of protein-protein associations. bioRxiv. 2023;: pubmed publisher
product information
master code :
10464-BA
SKU :
10464-BA-100
product name :
Recombinant E. coli BirA His-tag Protein, CF
unit size :
100 ug
description :
The Recombinant E. coli BirA His-tag Protein, CF from R&D Systems is derived from E. coli. The Recombinant E. coli BirA His-tag Protein, CF has been validated for the following applications: Enzyme Activity.
target :
BirA
category :
Proteins and Enzymes
buffer :
Supplied as a 0.2 ╡m filtered solution in MES, NaCl, TCEP, EDTA and Glycerol.
conjugate :
Unconjugated
purity :
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
species :
E. coli
observed molecular weight :
30-38 kDa, under reducing conditions
theoretical molecular weight :
36 kDa
details of functionality :
Measured by its ability to generate pyrophosphate from the biotinylation reaction. The pyrophosphate is subsequently hydrolyzed using Recombinant Yeast Inorganic Pyrophosphatase/PPA1 (ryPPA1) (Catalog # 8088-PP ). The specific activity is 10.0 pmol/min/╡g, as measured under the described conditions.
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
top caption :
Recombinant E. coli BirA His-tag Protein SDS-PAGE
accessionNumbers :
NP_418404.1
applications :
Enzyme Activity
source long :
E. coli -derived e. coli BirA protein Lys2-Lys321, with an N-terminal Met & 6-His tag
source short :
E. coli
USD :
425 USD
alt names :
Bifunctional protein birA, Bio R, BioR, Biotin [acetyl CoA carboxylase] synthetase, dhbB, ECK3965
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.