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Phospho-Ribosomal Protein S6 / RPS6 (S235 / S236) Antibody
R&D Systems
catalog: AF3918
rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry
citations: 2

Ribosomal Protein S6 in Human Colon Cancer Tissue. Ribosomal Protein S6 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using 5 ╡g/mL Rabbit Anti-Human/Mouse/Rat Phospho-Ribosomal Protein S6 (S235/S236) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3918) overnight at 4 ░C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell Tissue Staining Kit (brown; Catalog # CTS005 ) and counterstained with hematoxylin (blue). Specific labeling was localized to vascular endothelial cell within glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .
quantity: 100 ug (also 25 ug)
price: 299 USD
to the supplier
Ribosomal Protein S6 / RPS6 Antibody
R&D Systems
catalog: MAB5436
mouse monoclonal (522731)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
citations: 1

Detection of Human and Rat Ribosomal Protein S6 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.25 ╡g/mL of Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6 Monoclonal Antibody (Catalog # MAB5436) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). A specific band was detected for Ribosomal Protein S6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3 .
quantity: 100 ug (also 25 ug)
price: 319 USD
to the supplier
Ribosomal Protein S6 / RPS6 Antibody
R&D Systems
catalog: AF5436
sheep polyclonal
reactivity: human, mouse, rat
application: western blot

Detection of Human, Mouse, and Rat Ribosomal Protein S6 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 ╡g/mL of Sheep Anti-Human/Mouse/Rat Ribosomal Protein S6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5436) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for Ribosomal Protein S6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
quantity: 100 ug (also 25 ug)
price: 385 USD
to the supplier
Ribosomal Protein S6 / RPS6 Alexa Fluor 488-conjugated Antibody
R&D Systems
catalog: IC5436G
mouse monoclonal (522731)
reactivity: human, mouse, rat
conjugate: AF488
application: immunocytochemistry

Ribosomal Protein S6/RPS6 in HeLa Human Cell Line. Ribosomal Protein S6/RPS6 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6/RPS6 Alexa Fluor« 488-conjugated Monoclonal Antibody (Catalog # IC5436G) at 1:10 dilution for 3 hours at room temperature and counterstained with DAPI (blue). Specific staining was localized to ribosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips .
quantity: 100 Tests
price: 339 USD
to the supplier
 
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last updated:2020-01-04