Anti-Transglutaminase-3 (TG3) Antibody | QED Bioscience 90022 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Transglutaminase-3 (TG3) Antibody

catalog :

90022

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

C2D

The same clone is also sold as:

OriGene: AM10225PU-N

reactivity :

human

product information

CATALOG NUMBER :

90022

PRODUCT NAME :

Anti-Transglutaminase-3 (TG3) Antibody

NAME+SKU :

Anti-Transglutaminase-3 (TG3) Antibody (90022)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Transglutaminase-3 (TG3) Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Transglutaminase-3 (TG3)

SPECIES REACTIVITY :

Human

TARGET NAME :

Transglutaminase-3 (TG3)

UNIPROT :

Q08188

LOCATION :

Cytoplasm

PROTEIN NAME :

Protein-glutamine gamma-glutamyltransferase E

ALTERNATIVE NAMES :

EC 2.3.2.13, Transglutaminase E, TG(E, TGE, TGase E, Transglutaminase-3, TGase-3 [Cleaved into: Protein-glutamine gamma-glutamyltransferase E 50 kDa catalytic chain; Protein-glutamine gamma-glutamyltransferase E 27 kDa non-catalytic chain]

GENE NAME :

TGM3

CLONALITY :

Monoclonal

FORM :

Lyophilized

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

348.6

SKU SIZE 1 :

90022

APPLICATIONS :

WB, ICC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-5ug/ml. Bands of ~77kDa and ~30kDa are detected. Immunocytochemistry: use at 2-10ug/ml. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

C2D

IMMUNOGEN :

Recombinant human TG3 (accession no. Q08188).

RESEARCH AREA :

Enzymes

FORMULATION :

Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml.

BUFFER :

Tris Buffer

TARGET BACKGROUND :

Transglutaminase-3 (TG3), an epidermal- type enzyme, is responsible for formation of the epidermis. During keratinocyte differentiation, TG1 and TG3 appear to act cooperatively in the cross-linking of proteins, including involucrin, loricrin and small proline-rich proteins (SPRs). These reactions result in formation of the cornified envelope (CE), a specialized component consisting of covalent cross- links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Furthermore, TG3 in hair follicles is involved in cross-linking structural proteins such as trichohyalin and keratin intermediate to hardening the inner root sheath. During differentiation in these processes, a zymogen form of TG3 (77 kDa) is activated by limited proteolysis with cathepsins S and/or L.

FUNCTION :

Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath. {ECO:0000250}.

STORAGE CONDITION :

This product is stable for at least one (1) year at -20C to -70C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes full-length human TG3 (77kDa) and the proteolysed form of human TG3 (30-47kDa).

Image Name_1 :

qed-bioscience-anti-transglutaminase-3-tg3-antibody-90022-1.jpg

Applications 1 :

Image Name_2 :

qed-bioscience-anti-transglutaminase-3-tg3-antibody-90022-2.jpg

Applications 2 :

IHC