Anti-ALG-2 Interacting Protein-X (ALIX) Antibody | QED Bioscience 90001 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-ALG-2 Interacting Protein-X (ALIX) Antibody

catalog :

90001

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8E8

The same clone is also sold as:

OriGene: AM10558PU-N

reactivity :

human

product information

CATALOG NUMBER :

90001

PRODUCT NAME :

Anti-ALG-2 Interacting Protein-X (ALIX) Antibody

NAME+SKU :

Anti-ALG-2 Interacting Protein-X (ALIX) Antibody (90001)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-ALG-2 Interacting Protein-X (ALIX0 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

ALG-2 Interacting Protein-X (ALIX0

SPECIES REACTIVITY :

Human

TARGET NAME :

ALG-2 Interacting Protein-X (ALIX)

UNIPROT :

Q8WUM4

LOCATION :

Cytoplasm, cytosol , Melanosome , Cytoplasm, cytoskeleton, microtubule organizing center, centrosome , Secreted, extracellular exosome , Cell junction, tight junction , Midbody, Midbody ring

PROTEIN NAME :

Programmed cell death 6-interacting protein

ALTERNATIVE NAMES :

PDCD6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95

GENE NAME :

PDCD6IP

CLONALITY :

Monoclonal

FORM :

Lyophilized

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

348.6

SKU SIZE 1 :

90001

APPLICATIONS :

WB, IHC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-5ug/ml. A band of ~96kDa is detected. Immunohistochemistry: use at 5- 10ug/ml. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

8E8

IMMUNOGEN :

Recombinant human ALIX protein.

RESEARCH AREA :

Apoptosis

FORMULATION :

Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose.

BUFFER :

Tris Buffer

TARGET BACKGROUND :

ALG-2-interacting protein-X (ALIX), also known as AIP1, is a cytoplasmic protein ubiquitously expressed and concentrated in phagosomes and exosomes. ALIX may regulate apoptosis since it binds apoptosis-linked gene 2 (ALG-2), a Ca2+-binding protein necessary for cell death. Inhibiting ALG- 2 expression protects cells from death induced by several stimuli, an effect similar to that seen with overexpression of the C-terminal half of ALIX which contains the ALG-2-interacting domain.

FUNCTION :

Multifunctional protein involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complexes (PubMed:14739459). The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:17853893, PubMed:17556548). Adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis (PubMed:17853893, PubMed:17556548, PubMed:18641129). May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (PubMed:22660413). By interacting with F-actin, PARD3 and TJP1 secures the proper assembly and positioning of actomyosin-tight junction complex at the apical sides of adjacent epithelial cells that defines a spatial membrane domain essential for the maintenance of epithelial cell polarity and barrier (By similarity). {UniProtKB:Q9WU78, PubMed:14739459, PubMed:17556548, PubMed:17853893, PubMed:18641129, PubMed:22660413}.; (Microbial infection) Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function requires the interaction with CHMP4B. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as enveloped virus budding (HIV-1 and other lentiviruses). {PubMed:14505569, PubMed:14505570, PubMed:14519844, PubMed:17556548, PubMed:18641129}.

STORAGE CONDITION :

This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes human ALIX protein.