Anti-Transcription Factor IIB Antibody | QED Bioscience 70103 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Transcription Factor IIB Antibody

catalog :

70103

quantity :

200 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

IIB8

The same clone is also sold as:

Santa Cruz Biotechnology: sc-23875 X, sc-23875
Invitrogen: MA1-10884

reactivity :

human, mouse

product information

CATALOG NUMBER :

70103

PRODUCT NAME :

Anti-Transcription Factor IIB Antibody

NAME+SKU :

Anti-Transcription Factor IIB Antibody (70103)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Transcription Factor IIB Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Transcription Factor IIB

SPECIES REACTIVITY :

Human, mouse

TARGET NAME :

Transcription Factor IIB

UNIPROT :

Q00403

LOCATION :

Nucleus , Chromosome

PROTEIN NAME :

Transcription initiation factor IIB

ALTERNATIVE NAMES :

EC 2.3.1.48, General transcription factor TFIIB, S300-II

GENE NAME :

GTF2B

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

200 ug

DISTIRBUTOR PRICE :

285.6

SKU SIZE 1 :

70103

APPLICATIONS :

WB, ELISA, Gel Shift, TFIIB depletion, TFIIB inhibition

APPLICATION INSTRUCTIONS :

This antibody may be used in ELISA, Western blot, or electrophoretic mobility (gel shift) assays to identify human TFIIB. It can also immuno-deplet TFIIB from cell nuclear extracts and inhibit transcription of TFIIB from cell nuclear extracts. This antibody is polyol-responsive and can be used for gentle purification of TFIIB.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG2a

CLONE ID :

IIB8

IMMUNOGEN :

Partially purified recombinant human transcription factor IIB (TFIIB, Promega Corp.).

RESEARCH AREA :

Protein Transcription

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

FUNCTION :

General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-rPubMed:10318856, PubMed:10619841, PubMed:12931194, PubMed:1517211, PubMed:16230532, PubMed:1876184, PubMed:1946368, PubMed:24441171, PubMed:27193682, PubMed:3029109, PubMed:3818643, PubMed:7601352, PubMed:7675079, PubMed:8413225, PubMed:8504927, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:9420329}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C to -70C. Store product in appropriate aliquots to avoid multiple freeze- thaw cycles.

SPECIFICITY :

This antibody recognizes an epitope within amino acids 52-105 of human and mouse TFIIB.