Anti-TATA Binding Protein Antibody | QED Bioscience 70102 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-TATA Binding Protein Antibody

catalog :

70102

quantity :

200 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1TBP18

The same clone is also sold as:

Abcam: ab818
Invitrogen: MA1-21516, MA1-10883, MA1-189
Santa Cruz Biotechnology: sc-56795

reactivity :

human, mouse, rat

application :

western blot

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig 4b	Zhao T, Hong Y, Yin P, Li S, Li X. Differential HspBP1 expression accounts for the greater vulnerability of neurons than astrocytes to misfolded proteins. Proc Natl Acad Sci U S A. 2017;114:E7803-E7811 pubmed publisher

product information

CATALOG NUMBER :

70102

PRODUCT NAME :

Anti-TATA Binding Protein Antibody

NAME+SKU :

Anti-TATA Binding Protein Antibody (70102)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-TATA - Binding Protein Mouse Monoclonal Antibody

SPECIFICITY SHORT :

TATA - Binding Protein

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

TATA Binding Protein

UNIPROT :

P20226

LOCATION :

Nucleus

PROTEIN NAME :

TATA-box-binding protein

ALTERNATIVE NAMES :

TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit

GENE NAME :

TBP

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

200 ug

DISTIRBUTOR PRICE :

285.6

SKU SIZE 1 :

70102

APPLICATIONS :

WB, IP, Gel Shift

APPLICATION INSTRUCTIONS :

This antibody may be used in Western blot and electrophoretic mobility (gel shift) assays to detect mammalian TBP. It may also be used to immunoprecipitate a truncated TBP molecule containing only the N-terminal domain.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG1

CLONE ID :

1TBP18

IMMUNOGEN :

Recombinant human TBP.

RESEARCH AREA :

Protein Transcription

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

TARGET BACKGROUND :

The TATA-binding protein (TBP) plays a central role in the assembly of most eukaryotic transcription initiation complexes. TBP assembles with other proteins to form unique multimeric complexes for each of the three different nuclear RNA polymerases. TBP has been cloned from a variety of species and consists of two distinct domains. The C-terminal domain (~180 amino acids) is highly conserved among species, whereas the N-terminal domain varies considerably in length and sequence among different species. The conserved C-terminal domain contains the DNA-binding region as well as regions that interact with positive and negative regulatory proteins. In human TBP, the non-conserved N-terminal domain includes a stretch of glutamine residues ranging from 26 to 42. Several research groups have found an association between expansion of this polyglutamine tract with rare forms of spinocerebellar ataxia and other neurodegenerative diseases including Huntingtonâ€™s disease.

FUNCTION :

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription (PubMed:15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. {PubMed:15970593, PubMed:2194289, PubMed:2363050, PubMed:2374612, PubMed:26638071, PubMed:27193682, PubMed:9836642, ECO:0000305}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

These antibodies are stable for at least one (1) year at -20C to -70C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes an epitope within amino acid residues 1-20 of human, mouse and rat TBP. It does not react with TBP from Drosophila, yeast, silk worm, or Xenopus. Other species not investigated.

Image Name_1 :

qed-bioscience-anti-tata-binding-protein-antibody-70102-1.jpg

Applications 1 :