Anti-RNA Polymerase II Antibody | QED Bioscience 70101 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-RNA Polymerase II Antibody

catalog :

70101

quantity :

200 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8WG16

The same clone is also sold as:

Invitrogen: MA1-10882, MA1-26249
Santa Cruz Biotechnology: sc-56767, sc-56767 HRP, sc-56767 AC
BioLegend: 664913, 664916, 664908, 664906, 664915, 664914

reactivity :

human, mouse

citations: 1

Published Application/Species/Sample/Dilution	Reference
ChIP-Seq; mouse; loading ...; fig 6e	Xue Y, Pradhan S, Sun F, Chronis C, Tran N, Su T, et al. Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals. Mol Cell. 2017;67:594-607.e4 pubmed publisher

product information

CATALOG NUMBER :

70101

PRODUCT NAME :

Anti-RNA Polymerase II Antibody

NAME+SKU :

Anti-RNA Polymerase II Antibody (70101)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-RNA Polymerase II Mouse Monoclonal Antibody

SPECIFICITY SHORT :

RNA Polymerase II

SPECIES REACTIVITY :

Eukaryotic

TARGET NAME :

RNA Polymerase II

UNIPROT :

P24928

LOCATION :

Nucleus , Cytoplasm , Chromosome

PROTEIN NAME :

DNA-directed RNA polymerase II subunit RPB1

ALTERNATIVE NAMES :

RNA polymerase II subunit B1, EC 2.7.7.6, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, EC 2.7.7.48

GENE NAME :

POLR2A

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

200 ug

DISTIRBUTOR PRICE :

285.6

SKU SIZE 1 :

70101

APPLICATIONS :

WB, AP

APPLICATION INSTRUCTIONS :

This antibody may be used to detect unproteolyzed RNA polymerase II or to inhibit specific transcription from class II promoters. This is a polyol-responsive antibody and can be used in gentle purifications of RNA polymerase II from from wheat germ, calf thymus, and yeast and is likely to purify RNA polymerase II from most eukaryotic organisms.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG2a

CLONE ID :

8WG16

IMMUNOGEN :

Purified wheat germ RNA polymerase II.

RESEARCH AREA :

Protein Transcription

FORMULATION :

PBS, pH 7.4.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

FUNCTION :

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the sUniProtKB:P08775, PubMed:20231364, PubMed:23748380, PubMed:24207025, PubMed:26124092, PubMed:9852112}.; (Microbial infection) Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome. {PubMed:18032511}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.

SPECIFICITY :

This antibody reacts with the highly conserved heptapeptide repeat of the largest subunit of eukaryotic RNA polymerase II. It inhibits promoter- directed transcription, but it does not inhibit elongation in the nonspecific transcription assay.