Anti-Activating Transcription Factor 4 (ATF4) Antibody | QED Bioscience 56583 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Activating Transcription Factor 4 (ATF4) Antibody

catalog :

56583

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S360A-24

The same clone is also sold as:

Rockland Immunochemicals: 200-361-W61, 200-302-W61, 200-308-W61, 200-365-W61, 200-362-W61, 200-358-W61, 200-360-W61, 200-366-W61, 200-367-W61, 200-303-W61, 200-355-W61, 200-306-W61, 200-363-W61, 200-305-W61, 200-357-W61, 200-352-W61, 200-301-W61, 200-326-W61
Novus Biologicals: NBP2-42176
OriGene: AM60070PU-N

reactivity :

human, rat

product information

CATALOG NUMBER :

56583

PRODUCT NAME :

Anti-Activating Transcription Factor 4 (ATF4) Antibody

NAME+SKU :

Anti-Activating Transcription Factor 4 (ATF4) Antibody (56583)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Activating Transcription Factor 4 (ATF4) Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Activating Transcription Factor 4 (ATF4)

SPECIES REACTIVITY :

Human, rat

TARGET NAME :

Activating Transcription Factor 4 (ATF4)

UNIPROT :

P18848

LOCATION :

Nucleus , Nucleus speckle , Cytoplasm , Cell membrane , Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

PROTEIN NAME :

Cyclic AMP-dependent transcription factor ATF-4

ALTERNATIVE NAMES :

cAMP-dependent transcription factor ATF-4, Activating transcription factor 4, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding protein 2, Tax-responsive enhancer element-binding protein 67, TaxREB67

GENE NAME :

ATF4

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

56583

APPLICATIONS :

WB, IF

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-5ug/ml. A band of ~60kDa is detected. Immunofluorescence: use at 10ug/ml. Detection of ATF4 in rat brain lysate with #56583. These are recommended concentrations. Endusers should determine optimal concentrations for their application.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG2a

CLONE ID :

S360A-24

IMMUNOGEN :

Fusion protein corresponding to aa 25-327 of human ATF4. This sequence is 86

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.1% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

ATF4 belongs to the large ATF/CREB family of transcription factors. These proteins bind DNA via their basic region and dimerize via their leucine zipper domain to form a variety of homo- and heterodimers that regulate gene transcription. Two alternative transcripts encoding the same protein have been described. ATF4 transcription factor is also known to play role in osteoblast differentiation. Terminal osteoblast differentiation, is significantly inhibited by the inactivation of JNK; JNK inactivation downregulates expression of ATF-4.

FUNCTION :

Transcription factor that binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3') and displays two biological functions, as regulator of metabolic and redox processes under normal cellular conditions, and as master transcription factor during integrated stress response (ISR) (PubMed:17684156, PubMed:16682973, PubMed:31444471, PubMed:32132707). Binds to asymmetric CRE's as a heterodimer and to palindromic CRE's as a homodimer (By similarity). Core effector of the ISR, which is required for adaptation to various stress such as endoplasmic reticulum (ER) stress, amino acid starvation, mitochondrial stress or oxidative stress (PubMed:32132707). During ISR, ATF4 translation is induced via an alternative ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced ATF4 acts as a master transcription factor of stress-responsive genes in order to promote cell rUniProtKB:Q06507, PubMed:11960987, PubMed:15109498, PubMed:15775988, PubMed:16682973, PubMed:17684156, PubMed:18940792, PubMed:26086088, PubMed:31444471, PubMed:32132706, PubMed:32132707, PubMed:33384352}.; (Microbial infection) Binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. {PubMed:1847461}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE CONDITION :

This product is stable for at least one (1) year at -20C.

SPECIFICITY :

This antibody recognizes human and rat ATF4.

Image Name_1 :

qed-bioscience-anti-activating-transcription-factor-4-atf4-antibody-56583-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-activating-transcription-factor-4-atf4-antibody-56583-2.png

Applications 2 :

Western Blot

Image Name_3 :

qed-bioscience-anti-activating-transcription-factor-4-atf4-antibody-56583-3.png

Applications 3 :

ICC