Anti-VDAC1 Antibody | QED Bioscience 56564 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-VDAC1 Antibody

catalog :

56564

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S152B-23

The same clone is also sold as:

Novus Biologicals: NBP2-42187
Rockland Immunochemicals: 200-360-W70, 200-352-W70, 200-302-W70, 200-303-W70, 200-357-W70, 200-306-W70, 200-363-W70, 200-326-W70, 200-358-W70, 200-361-W70, 200-362-W70, 200-355-W70, 200-365-W70, 200-305-W70, 200-367-W70, 200-366-W70, 200-308-W70

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

56564

PRODUCT NAME :

Anti-VDAC1 Antibody

NAME+SKU :

Anti-VDAC1 Antibody (56564)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-VDAC1 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

VDAC1

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

VDAC1

UNIPROT :

P21796

LOCATION :

Mitochondrion outer membrane , Cell membrane , Membrane raft , Multi-pass membrane protein

PROTEIN NAME :

Voltage-dependent anion-selective channel protein 1

ALTERNATIVE NAMES :

VDAC-1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM

GENE NAME :

VDAC1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

56564

APPLICATIONS :

WB, IF

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-5ug/ml. A band of ~30kDa is detected. Immunofluorescence: use at 10ug/ml These are recommended concentrations. Endusers should determine optimal concentrations for their application.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG2a

CLONE ID :

S152B-23

IMMUNOGEN :

Fusion protein corresponding to aa 1-283 (full-length) of human VDAC1. This sequence is 98

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.1% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

VDAC1 (also known as Voltage-dependent anion-selective channel protein 1 and outer mitochondrial membrane protein porin 1) is the outer mitochondrial membrane receptor for hexokinase and BCL2L1. VDAC1 forms a channel through the mitochondrial membrane and is involved in small molecule diffusion, cell volume regulation, and apoptosis. VDAC1 may participate in the formation of the permeability transition pore complex (PTPC) which is responsible for the release of mitochondrial products that trigger apoptosis.

FUNCTION :

Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:11845315, PubMed:18755977, PubMed:20230784, PubMed:8420959). Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterol cholesterol (PubMed:31015432). In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed:32047033). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756). May mediate ATP export from cells (PubMed:30061676). {PubMed:11845315, PubMed:15033708, PubMed:18755977, PubMed:20230784, PubMed:25296756, PubMed:30061676, PubMed:31015432, PubMed:32047033, PubMed:8420959}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE CONDITION :

This product is stable for at least one (1) year at -20C.

SPECIFICITY :

This antibody recognizes human, mouse and rat VDAC1. It does not cross-react with VDAC2 or VDAC3 (based on evaluation in knock-out mice).

Image Name_1 :

qed-bioscience-anti-vdac1-antibody-56564-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-vdac1-antibody-56564-2.png

Applications 2 :

ICC

Image Name_3 :

qed-bioscience-anti-vdac1-antibody-56564-3.png

Applications 3 :

Western Blot