Anti-Mitofusin-2 Antibody | QED Bioscience 56557 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Mitofusin-2 Antibody

catalog :

56557

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S153-5

The same clone is also sold as:

OriGene: AM60065PU-N
Novus Biologicals: NBP2-42188
Rockland Immunochemicals: 200-352-W71, 200-357-W71, 200-308-W71, 200-305-W71, 200-361-W71, 200-326-W71, 200-363-W71, 200-306-W71, 200-366-W71, 200-358-W71, 200-355-W71, 200-360-W71, 200-362-W71, 200-367-W71, 200-303-W71, 200-302-W71, 200-365-W71, 200-301-W71

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

56557

PRODUCT NAME :

Anti-Mitofusin-2 Antibody

NAME+SKU :

Anti-Mitofusin-2 Antibody (56557)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Mitofusin-2 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Mitofusin-2

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

Mitofusin-2

UNIPROT :

Q80U63

LOCATION :

Mitochondrion outer membrane

PROTEIN NAME :

Mitofusin-2

ALTERNATIVE NAMES :

EC 3.6.5.-, Hypertension-related protein 1, Mitochondrial assembly regulatory factor, HSG protein, Transmembrane GTPase MFN2

GENE NAME :

Mfn2

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

56557

APPLICATIONS :

WB, IF

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-5ug/ml. A band of ~90kDa is detected. Immunofluorescence: use at 10ug/ml. These are recommended concentrations. Enduser should determine optimal concentrations for their application.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by Protein G affinity chromatography.

ISOTYPE :

IgG2a

CLONE ID :

S153-5

IMMUNOGEN :

Fusion protein corresponding to aa 370-600 (cytoplasmic N-terminus) of mouse Mitofusin-2. This sequence is 97

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.1% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Mitofusin-2 (Mfn2) is a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2 and hereditary motor and sensory neuropathy VI, both of which are disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.

FUNCTION :

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12527753, PubMed:23921378, PubMed:23620051). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (By similarity). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Plays an important role in the regulation of vascular smooth muscle cell proliferation (By similarity). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria (PubMed:23620051). Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (PubMed:23921556). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (PubMed:23921556). {UniProtKB:O95140, UniProtKB:Q8R500, PubMed:12527753, PubMed:23620051, PubMed:23921378, PubMed:23921556}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE CONDITION :

This product is stable for at least one (1) year at -20C.

SPECIFICITY :

This antibody recognizes human, mouse, and rat Mitofusin-2. It does not cross- react with Mitofusin-1.

Image Name_1 :

qed-bioscience-anti-mitofusin-2-antibody-56557-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-mitofusin-2-antibody-56557-2.png

Applications 2 :

ICC

Image Name_3 :

qed-bioscience-anti-mitofusin-2-antibody-56557-3.png

Applications 3 :

Western Blot