Anti-GluN2A/NR2A Antibody | QED Bioscience 56546 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-GluN2A/NR2A Antibody

catalog :

56546

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S327-95

The same clone is also sold as:

OriGene: TA326537
Abcam: ab240884
Novus Biologicals: NBP2-22405

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

56546

PRODUCT NAME :

Anti-GluN2A/NR2A Antibody

NAME+SKU :

Anti-GluN2A/NR2A Antibody (56546)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-GluN2A/NR2A Mouse Monoclonal Antibody

SPECIFICITY SHORT :

GluN2A/NR2A

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

GluN2A/NR2A

UNIPROT :

Q00959

LOCATION :

Cell projection, dendritic spine , Cell membrane , Cell junction, synapse , Cell junction, synapse, postsynaptic cell membrane , Cytoplasmic vesicle membrane

PROTEIN NAME :

Glutamate receptor ionotropic, NMDA 2A

ALTERNATIVE NAMES :

GluN2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NMDAR2A, NR2A

GENE NAME :

Grin2a

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

56546

APPLICATIONS :

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1ug/ml. A band of ~170kDa is detected. Positive control: Rat brain lysate. These are recommended concentrations. User should determine optimal concentrations for their application.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG2a

CLONE ID :

S327-95

IMMUNOGEN :

Fusion protein corresponding to aa 75-325 (extracellular N-terminus) of rat GluN2A/NR2A (accession no. Q00959).

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

N-methyl-D-aspartate receptors are heteromeric protein complexes, and three families of NMDAR subunits have been identified: NR1, NR2 and NR3. The subunit composition determines the pharmacological and physiological properties of the NMDARs. NR2A is gradually increased in adult CNS but is absent in embryonic CNS. Electro- physiological data suggest that NR2A- containing NMDARs tend to be localized synaptically, whereas NR2B-containing receptors tend to be localized peri- synaptically or extrasynaptically. In the spinal cord, the predominant subunits of NMDARs include NR2A at synaptic sites and NR2B at extrasynaptic sites.

FUNCTION :

Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition; channels containing GRIN1 and GRIN2A have lower sensitivity to glutamate and faster deactivation kinetics than channels formed by GRIN1 and GRIN2B (PubMed:28384476). Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity). {UniProtKB:P35436, PubMed:1350383, PubMed:16281028, PubMed:23625947, PubMed:24462099, PubMed:26679993, PubMed:27618671, PubMed:27916457, PubMed:28384476, PubMed:28468946, PubMed:28760974, PubMed:8428958}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes human, mouse, and rat GluN2A/NR2A. It does not cross- react with NR2B.

Image Name_1 :

qed-bioscience-anti-glun2a-nr2a-antibody-56546-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-glun2a-nr2a-antibody-56546-2.png

Applications 2 :

ICC

Image Name_3 :

qed-bioscience-anti-glun2a-nr2a-antibody-56546-3.png

Applications 3 :

Western Blot