Anti-Notch1 Antibody | QED Bioscience 56544 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Notch1 Antibody

catalog :

56544

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S253-32

The same clone is also sold as:

OriGene: AM60030PU-N

reactivity :

mouse, rat

product information

CATALOG NUMBER :

56544

PRODUCT NAME :

Anti-Notch1 Antibody

NAME+SKU :

Anti-Notch1 Antibody (56544)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Notch1 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Notch1

SPECIES REACTIVITY :

Mouse, rat

TARGET NAME :

Notch1

UNIPROT :

Q01705

LOCATION :

Cell membrane

PROTEIN NAME :

Neurogenic locus notch homolog protein 1

ALTERNATIVE NAMES :

Notch 1, Motch A, mT14, p300 [Cleaved into: Notch 1 extracellular truncation

GENE NAME :

Notch1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

Lot Specific

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

56544

APPLICATIONS :

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1ug/ml. Bands of >270kDa, ~120kDa, and smaller fragments (due to proteolysis) are detected . Positive control: Rat brain lysate. These are recommended concentrations. User should determine optimal concentrations for their application.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

S253-32

IMMUNOGEN :

Fusion protein corresponding to aa 20-216 (extracellular N-terminus, EGF- like domains 1-5) of mouse Notch1 (accession no. Q01705).

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Members of the Notch trans-membrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. The Notch signaling network is an intercellular signaling pathway that regulates interactions between physically adjacent cells. The Notch1 protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. It functions as a receptor for membrane bound ligands, and may play multiple roles during development. There is evidence that activated Notch 1 and Notch 3 promote differentiation of progenitor cells into astroglia. Notch 1, activated before birth, induces radial glia differentiation,but postnatally induces the differentiation into astrocytes.

FUNCTION :

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). {PubMed:15965470, PubMed:18299578, PubMed:23160044, PubMed:23615612}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

SPECIFICITY :

This antibody recognizes mouse and rat Notch1. It does not cross-react with Notch2 or Notch3.

Image Name_1 :

qed-bioscience-anti-notch1-antibody-56544-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-notch1-antibody-56544-2.png

Applications 2 :

Western Blot

Image Name_3 :

qed-bioscience-anti-notch1-antibody-56544-3.png

Applications 3 :

ICC