Anti-LRP4 (extracellular) Antibody | QED Bioscience 56540 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-LRP4 (extracellular) Antibody

catalog :

56540

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S207-27

The same clone is also sold as:

Novus Biologicals: NBP2-22416
OriGene: TA326569

reactivity :

mouse, rat

product information

CATALOG NUMBER :

56540

PRODUCT NAME :

Anti-LRP4 (extracellular) Antibody

NAME+SKU :

Anti-LRP4 (extracellular) Antibody (56540)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-LRP4 (extracellular) Mouse Monoclonal Antibody

SPECIFICITY SHORT :

LRP4 (extracellular)

SPECIES REACTIVITY :

Mouse, rat

TARGET NAME :

LRP4 (extracellular)

UNIPROT :

Q8VI56

LOCATION :

Cell membrane , Single-pass type I membrane protein

PROTEIN NAME :

Low-density lipoprotein receptor-related protein 4

ALTERNATIVE NAMES :

LRP-4, LDLR dan

GENE NAME :

Lrp4

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

56540

APPLICATIONS :

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1ug/ml. Predicted molecular weight is ~215kDa. Degradation products of approx. 150kDa are also detected. Positive Control: rat brain lysate. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG2a

CLONE ID :

S207-27

IMMUNOGEN :

Fusion protein corresponding to aa 26-350 (extracellular N-terminus) of mouse LRP4 (accession no.Q8V156).

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

The formation of neuromuscular junctions (NMJ) requires the interaction between motor neurons and muscle fibers. LRP4 (low-density lipoprotein receptor-related protein 4) is a receptor of agrin that is thought to act in cis to stimulate MuSK in muscle fibers for postsynaptic differentiation. Recent studies of muscle- specific mutants suggest that LRP4 is involved in determining where AChR clusters form in muscle fibers, postsynaptic differentiation, and axon terminal development.

FUNCTION :

Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN-induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. Plays an essential role in the process of digit differentiation (PubMed:16517118). {PubMed:16517118, PubMed:18848351, PubMed:21471202}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This antibody is stable for at least one (1) year at -20C. Avoid repeated freeze- thaw cycles.

SPECIFICITY :

This antibody recognizes mouse and rat LRP4.

Image Name_1 :

qed-bioscience-anti-lrp4-extracellular-antibody-56540-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-lrp4-extracellular-antibody-56540-2.png

Applications 2 :

Western Blot

Image Name_3 :

qed-bioscience-anti-lrp4-extracellular-antibody-56540-3.png

Applications 3 :

ICC

Image Name_4 :

qed-bioscience-anti-lrp4-extracellular-antibody-56540-4.png

Image Caption_4 :

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody, Clone S207-27 (56540). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody (56540) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) LRP4 (Extracellular) Antibody. (D) Composite.

Applications 4 :

ICC