Anti-GABA(B)R1 Antibody | QED Bioscience 56524 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-GABA(B)R1 Antibody

catalog :

56524

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S93A-49

The same clone is also sold as:

OriGene: TA326530

reactivity :

human, mouse, rat

product information

CATALOG NUMBER :

56524

PRODUCT NAME :

Anti-GABA(B)R1 Antibody

NAME+SKU :

Anti-GABA(B)R1 Antibody (56524)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-GABA(B)R1 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

GABA(B)R1

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

GABA(B)R1

UNIPROT :

Q9Z0U4

LOCATION :

Cell membrane , Cell junction, synapse, postsynaptic cell membrane , Cell projection, dendrite , Perikaryon

PROTEIN NAME :

Gamma-aminobutyric acid type B receptor subunit 1

ALTERNATIVE NAMES :

GABA-B receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1

GENE NAME :

Gabbr1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

56524

APPLICATIONS :

WB, IHC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-2ug/ml. A band of ~115kDa is detected. Immunocytochemistry: use at 1-5ug/ml. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Rat brain membranes.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

S93A-49

IMMUNOGEN :

Fusion protein corresponding to aa 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1 (accession no. NP_112290.2).

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

GABA (gamma-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B), and GABA(C). GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission. Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 in which GABA(B)R1 binds a ligand and GABA(B)R2 is the primary G protein contact site.

FUNCTION :

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:9872315, PubMed:9872317, PubMed:9872744). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed:9872317, PubMed:10658574). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10075644, PubMed:9872315, PubMed:9872744, PubMed:10924501). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:9069281, PubMed:10457184, PubMed:9872315, PubMed:9872744, PubMed:10924501, PubMed:10692480). Calcium is required for high affinity binding to GABA (PubMed:10692480). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed:9872744). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:9872744, PubMed:10924501, PubMed:10692480). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (By similarity). {UniProtKB:Q9UBS5, PubMed:10075644, PubMed:10457184, PubMed:10658574, PubMed:10692480, PubMed:10924501, PubMed:9069281, PubMed:9872315, PubMed:9872744}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This product is stable for at least 1 year at -20C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes human, mouse, and rat GABA(B)R1. It does not cross- react with GABA(B)R2.

Image Name_1 :

qed-bioscience-anti-gababr1-antibody-56524-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-gababr1-antibody-56524-2.png

Applications 2 :

Western Blot

Image Name_3 :

qed-bioscience-anti-gababr1-antibody-56524-3.png

Applications 3 :

ICC