Anti-EAAC1 Antibody | QED Bioscience 56520 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-EAAC1 Antibody

catalog :

56520

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S180-41

The same clone is also sold as:

Novus Biologicals: NBP2-59319
OriGene: TA326381

reactivity :

human

product information

CATALOG NUMBER :

56520

PRODUCT NAME :

Anti-EAAC1 Antibody

NAME+SKU :

Anti-EAAC1 Antibody (56520)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-EAAC1 Mouse Monoclonal Antibody

SPECIFICITY SHORT :

EAAC1

SPECIES REACTIVITY :

Human

TARGET NAME :

EAAC1

UNIPROT :

P43005

LOCATION :

Cell membrane , Apical cell membrane , Cell junction, synapse, synaptosome , Early endosome membrane , Late endosome membrane , Recycling endosome membrane

PROTEIN NAME :

Excitatory amino acid transporter 3

ALTERNATIVE NAMES :

Excitatory amino-acid carrier 1, Neuronal and epithelial glutamate transporter, Sodium-dependent glutamate/aspartate transporter 3, Solute carrier family 1 member 1

GENE NAME :

SLC1A1

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

56520

APPLICATIONS :

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-5ug/ml. A band of ~60kDa is detected. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain membranes.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

S180-41

IMMUNOGEN :

Fusion protein corresponding to aa 1-524 (full-length) of human EAAC1 (accession no. NP_004161.4).

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Excitatory amino-acid carrier 1 (EAAC1) is a high-affinity Na+-dependent L- glutamate/D,L-aspartate cell membrane transport protein. It is expressed in brain as well as in several non-nervous system tissues. In brain, EAAC1 is the primary neuronal glutamate transporter. It has a polarized distribution in cells and functions mainly perisynaptically to transport glutamate from the extracellular environment. In the kidney it is involved in renal acidic amino acid reabsorption and amino acid metabolism. EAAC1- associated protein GTRAP3-18 may be important in regulating the metabolic function of EAAC1.

FUNCTION :

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7914198, PubMed:7521911, PubMed:8857541, PubMed:26690923, PubMed:21123949). Can also transport L-cysteine (PubMed:21123949). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:7521911, PubMed:8857541, PubMed:26690923). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed:8857541, PubMed:26690923). Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli (PubMed:21123949). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity). Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport (By similarity). Negatively regulated by ARL6IP5 (By similarity). {UniProtKB:P51906, UniProtKB:P51907, PubMed:21123949, PubMed:26690923, PubMed:7521911, PubMed:7914198, PubMed:8857541}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This product is stable for at least 1 year at -20C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes human EAAC1.

Image Name_1 :

qed-bioscience-anti-eaac1-antibody-56520-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-eaac1-antibody-56520-2.png

Applications 2 :

ICC

Image Name_3 :

qed-bioscience-anti-eaac1-antibody-56520-3.png

Applications 3 :

Western Blot