Anti-Ankyrin-B Antibody | QED Bioscience 56518 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

QED Bioscience

product type :

antibody

product name :

Anti-Ankyrin-B Antibody

catalog :

56518

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S105-13

The same clone is also sold as:

OriGene: TA326443
Novus Biologicals: NBP2-59374

reactivity :

human, rat

product information

CATALOG NUMBER :

56518

PRODUCT NAME :

Anti-Ankyrin-B Antibody

NAME+SKU :

Anti-Ankyrin-B Antibody (56518)

PRODUCT _TYPE :

Antibody

DESCRIPTION :

Anti-Ankyrin-B Mouse Monoclonal Antibody

SPECIFICITY SHORT :

Ankyrin-B

SPECIES REACTIVITY :

Human, mouse, rat

TARGET NAME :

Ankyrin-B

UNIPROT :

Q01484

LOCATION :

Cytoplasm, cytoskeleton , Membrane , Cytoplasm, myofibril, sarcomere, M line , Apical cell membrane , Cell membrane , Cell junction, synapse, postsynaptic cell membrane , Early endosome , Recycling endosome , Lysosome , Mitochondrion , Cytoplasm, myofibril, sarcomere, Z line , Cell membrane, sarcolemma, T-tubule

PROTEIN NAME :

Ankyrin-2

ALTERNATIVE NAMES :

ANK-2, Ankyrin-B, Brain ankyrin, Non-erythroid ankyrin

GENE NAME :

ANK2

CLONALITY :

Monoclonal

FORM :

Liquid

CONCENTRATION :

1.0 mg/mL

SIZE 1 :

100 ug

DISTIRBUTOR PRICE :

305.2

SKU SIZE 1 :

56518

APPLICATIONS :

WB, IHC

APPLICATION INSTRUCTIONS :

Immunoblotting: use at 1-2ug/ml. A band of >200kDa is detected. Immunohistochemistry: use at 1-5ug/ml. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain membranes.

HOST :

Mouse

FORMAT :

Purified

PURIFICATION :

Purified by immunoaffinity chromatography

ISOTYPE :

IgG1

CLONE ID :

S105-13

IMMUNOGEN :

Synthetic peptide correspond- ing to aa 203-496 of human Ankyrin-B (accession no. NP_001120965.1)

RESEARCH AREA :

Neuroscience

FORMULATION :

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

BUFFER :

Phosphate Buffered Saline

BUFFER PH :

pH 7.4

BUFFER ANTI-MICROBIAL :

0.09% Sodium Azide

BUFFER CRYOPRESERVATIVE :

50% Glycerol

TARGET BACKGROUND :

Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton. Ankyrins have binding sites for the beta subunit of spectrin and for at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers, and ion transporters in the plasma membrane.

FUNCTION :

Plays an essential role in the localization and membrane stabilization of ion transporters and ion channels in several cell types, including cardiomyocytes, as well as in striated muscle cells. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions. In cardiomyocytes, required for coordinate assembly of Na/Ca exchanger, SLC8A1/NCX1, Na/K ATPases ATP1A1 and ATP1A2 and inositol 1,4,5-trisphosphate (InsP3) receptors at sarcoplasmic reticulum/sarcolemma sites. Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate (PubMed:12571597). In the inner segment of rod photoreceptors, required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) (By similarity). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (By similarity). {UniProtKB:Q8C8R3, PubMed:12571597}.

DILUTION INSTRUCTIONS :

Dilute in PBS or medium which is identical to that used in the assay system.

STORAGE CONDITION :

This product is stable for at least 1 year at -20C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

SPECIFICITY :

This antibody recognizes human, mouse, and rat Ankyrin-B.

Image Name_1 :

qed-bioscience-anti-ankyrin-b-antibody-56518-1.png

Applications 1 :

ICC

Image Name_2 :

qed-bioscience-anti-ankyrin-b-antibody-56518-2.png

Applications 2 :

ICC

Image Name_3 :

qed-bioscience-anti-ankyrin-b-antibody-56518-3.png

Applications 3 :

ICC